Chitosan/nHAC/PLGA microsphere vehicle for sustained release of rhBMP‐2 and its derived synthetic oligopeptide for bone regeneration

Abstract

Both of the osteogenic factor and the suitable delivery system in bone tissue engineering are essential for bone regeneration. In this study, we manufactured two kinds of composite vehicles for sustained release of rhBMP-2 and its derived synthetic oligopeptide (Peptide-24, abbreviated as P24) for osteogenesis and bone defect repair. The composite vehicle was based on cross-linked chitosan, nano-hydroxyapatite/collagen (nHAC), and poly (lactide-co-glycolide) acid microsphere. The physicochemical properties of the composite vehicles (abbreviated as CS/nHAC/PLGA-MS) were investigated. The rhBMP-2 and P24 release kinetics from the vehicles were examined and the secondary structure of rhBMP-2 and P24 after 28 days' release process was analyzed. In vitro cell proliferation, osteogenic differentiation and rat calvarial defect repair were evaluated. The results proved that the composite vehicle had favorable compressive strength, elastic modulus, the porosity, and the bulk density. The secondary structures of rhBMP-2 and P24 kept stability during microencapsulation and release process. P24 from the vehicle kept a geared-up release and rhBMP-2 from the vehicle kept a three-stage mode release process. The results of in vitro cell detection showed that the composite vehicle had good biocompatibility and osteoinduction. In vivo rat calvarial defect repair demonstrated that both groups of vehicles with rhBMP2 and P24 exhibited satisfied bone defect repair. This research showed that the composite vehicle could exhibit sustained release of osteogenic factors. CS/nHAC/PLGA-MS loading rhBMP-2 or P24 could be a novel and ideal scaffold for bone regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1593-1606, 2017.

Simultaneous incorporation of PTH(1–34) and nano-hydroxyapatite into Chitosan/Alginate Hydrogels for efficient bone regeneration

“…Autogenous transplantation, the gold standard method for bone therapy, is limited for the lack of sources of autografts [ 7 , 8 ], besides it also has a risk of infection, nerve damage and other postoperative complications [ 9 ]. To address these challenges, researchers have focused on the development of novel biomaterials with potential in tissue engineering and regeneration to facilitate bone regeneration [ [10] , [11] , [12] , [13] ]. Among synthetic biomaterials, hydrogels consisting of natural or synthetic polymers that exhibit excellent mechanical properties and biocompatibility are ideal scaffolds to emulate extracellular matrices for cell proliferation and differentiation [ 12 , [14] , [15] , [16] , [17] ], thus leading to broad utilization in bone regeneration.…”

Statistical degradation modelling of Poly(D,L-lactide-co-glycolide) copolymers for bioscaffold applications

“…PDLGA, such as other biomaterials used for biomedical purposes, should be nontoxic (glycolic and lactic acids are easy metabolized by the body) [ 37 ], bioresorbable, able to attach the cells, promote their proliferation and differentiation, and also degrade according to physiological rates [ 38 , 39 ]. Thus, it is crucial to estimate the degree and rate of degradation of this type of materials, in order to apply the proper material depending of the application.…”

BMP9 promotes osteogenic differentiation of SMSCs by activating the JNK/Smad2/3 signaling pathway

“…It is known that HCP is an artificial composite material that promotes osteogenesis by osteoinduction and osteoconduction, and it has been considered as one of the most prospective materials for tissue engineering . Wang F et al demonstrated that the HCP scaffolds were the most suitable for adhesion, proliferation, and osteogenic differentiation of human umbilical cord mesenchymal stem cells in nude mouse subcutaneous implantation.…”