Chitosan Scaffold Containing Periostin Can Accelerate Bone Defect Regeneration in Non-Weight-Bearing Conditions

Barakzai, Shogoofa; Salehiamin, Mehdi; Shaban, Mohammad Omar; Azami, Mahmoud; Heidari, Sahar; Sobhani, Aligholi

doi:10.1007/s40883-022-00268-9

Cited by 2 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, the densest collagen deposition in alveolar sockets within the QDL hydrogel group reached 75.33%, 1.21 times higher than that in the control group. The toluidine blue staining to evaluate the structure of the defect area in bone defects in the proximal mesiobuccally roots of extraction sockets . There were more positive areas in the QDL hydrogel group compared to the control group, indicating more new bone formation in the QDL hydrogel group, and the result was consistent with the results of the Masson’s trichrome staining (Figure S12).…”

Section: Results and Discussionsupporting

confidence: 75%

“…The toluidine blue staining to evaluate the structure of the defect area in bone defects in the proximal mesiobuccally roots of extraction sockets. 50 There were more positive areas in the QDL hydrogel group compared to control group, indicating more new bone formation in the QDL hydrogel group, and the result was consistent with the results of the Masson's trichrome staining (Figure S12). These findings highlight a substantial acceleration in alveolar socket healing facilitated by the QDL hydrogel, attributed to its promotion of collagen deposition and woven bone formation.…”

Section: Solution S8supporting

confidence: 86%

“…The toluidine blue staining to evaluate the structure of the defect area in bone defects in the proximal mesiobuccally roots of extraction sockets. 50 S12). These findings highlight a substantial acceleration in alveolar socket healing facilitated by the QDL hydrogel, attributed to its promotion of collagen deposition and woven bone formation.…”

Section: Solution S8mentioning

confidence: 99%

See 2 more Smart Citations

Periodontium-Mimicking, Multifunctional Biomass-Based Hydrogel Promotes Full-Course Socket Healing

Guo,

Shao,

Wang

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

Preserving stable tooth-periodontal tissue integration is vital for maintaining alveolar bone stability under physiological conditions. However, tooth extraction compromises this integration and impedes socket healing. Therefore, it becomes crucial to provide early stage coverage of the socket to promote optimal healing. Drawing inspiration from the periodontium, we have developed a quaternized methacryloyl chitosan/dopaminegrafted oxidized sodium alginate hydrogel, termed the quaternized methacryloyl chitosan/dopamine-grafted oxidized sodium alginate hydrogel (QDL hydrogel). Through blue-light-induced crosslinking, the QDL hydrogel serves as a comprehensive wound dressing for socket healing. The QDL hydrogel exhibits remarkable efficacy in closing irregular tooth extraction wounds. Its favorable mechanical properties, flexible formability, and strong adhesion are achieved through modifications of chitosan and sodium alginate derived from biomass sources. Moreover, the QDL hydrogel demonstrates a superior hemostatic ability, facilitating swift blood clot formation. Additionally, the inherent antibacterial properties of the QDL hydrogel effectively inhibit oral microorganisms. Furthermore, the QDL hydrogel promotes angiogenesis, which facilitates the nutrient supply for subsequent tissue regeneration. Notably, the hydrogel accelerates socket healing by upregulating the expression of genes associated with wound healing. In conclusion, the periodontium-mimicking multifunctional hydrogel exhibits significant potential as a clinical tooth extraction wound dressing.

show abstract

Section: Results and Discussionsupporting

confidence: 75%

Section: Solution S8supporting

confidence: 86%

Section: Solution S8mentioning

confidence: 99%

See 1 more Smart Citation

Periodontium-Mimicking, Multifunctional Biomass-Based Hydrogel Promotes Full-Course Socket Healing

Guo,

Shao,

Wang

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…Possible synergistic effects of TAM inhibition may also be relevant for tissue engineering. Pairing of TAM inhibition with engineered scaffolds with previously studied extracellular matrix proteins (e.g., periostin) that promote Wnt activation may enhance bone formation after injury (Barakzai et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Mertk Signaling Enhances Bone Healing after Tooth Extraction

et al. 2023

View full text Add to dashboard Cite

Regeneration of alveolar bone is an essential step in restoring healthy function following tooth extraction. Growth of new bone in the healing extraction socket can be variable and often unpredictable when systemic comorbidities are present, leading to the need for additional therapeutic targets to accelerate the regenerative process. One such target is the TAM family (Tyro3, Axl, Mertk) of receptor tyrosine kinases. These proteins have been shown to help resolve inflammation and maintain bone homeostasis and thus may have therapeutic benefits in bone regeneration following extraction. Treatment of mice with a pan-TAM inhibitor (RXDX-106) led to accelerated alveolar bone fill following first molar extraction in a mouse model without changing immune infiltrate. Treatment of human alveolar bone mesenchymal stem cells with RXDX-106 upregulated Wnt signaling and primed the cells for osteogenic differentiation. Differentiation of human alveolar bone mesenchymal stem cells with osteogenic media and TAM-targeted inhibitor RXDX-106 (pan-TAM), ASP-2215 (Axl specific), or MRX-2843 (Mertk specific) showed enhanced mineralization with pan-TAM or Mertk-specific inhibitors and no change with Axl-specific inhibitor. First molar extractions in Mertk–/– mice had increased alveolar bone regeneration in the extraction socket relative to wild type controls 7 d postextraction. Flow cytometry of 7-d extraction sockets showed no difference in immune cell numbers between Mertk –/– and wild type mice. RNAseq of day 7 extraction sockets showed increased innate immune-related pathways and genes associated with bone differentiation in Mertk –/– mice. Together, these results indicate that TAM receptor signaling, specifically through Mertk, can be targeted to enhance bone regeneration after injury.

show abstract

Chitosan Scaffold Containing Periostin Can Accelerate Bone Defect Regeneration in Non-Weight-Bearing Conditions

Cited by 2 publications

References 42 publications

Periodontium-Mimicking, Multifunctional Biomass-Based Hydrogel Promotes Full-Course Socket Healing

Periodontium-Mimicking, Multifunctional Biomass-Based Hydrogel Promotes Full-Course Socket Healing

Inhibition of Mertk Signaling Enhances Bone Healing after Tooth Extraction

Contact Info

Product

Resources

About