Chitosan/Silk Sericin Blend Microparticles Prepared by Water-in-Oil Emulsification-Diffusion for Controlled Release of Silk Sericin Antioxidant

Abstract

This study aimed to develop the novel drug delivery carriers to control the release of silk sericin antioxidant. The chitosan/silk sericin blend microparticles were successfully prepared by the water-in-oil emulsification-diffusion method. Influence of silk sericin ratio on characteristics and in vitro silk sericin release behaviors of the blend microparticles was investigated. All the spherical-shaped blend microparticles had an average size of 41 – 48 m and could load the silk sericin in high loading efficiency (69 – 75%). The FTIR and thermogravimetric results indicated the blend microparticles with different silk sericin contents can be prepared by varying the silk sericin feed ratio. The blend microparticles exhibited sustained release profiles of the silk sericin. The in vitro silk sericin release content increased as the silk sericin ratio increased. Antioxidant activities of the blend microparticles assessed by FRAP assay were more than the neat chitosan microparticles and steadily increased with the silk sericin ratio. The results demonstrated that the chitosan/silk sericin blend microparticles prepared by the water-in-oil emulsification-diffusion method should be useful drug delivery carriers for the controlled release silk sericin antioxidant.

Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration

“…A water-in-oil emulsification method can be applied to develop SS-loaded microparticles. Phromsopha and Baimark [ 98 ] produced spherical-shaped chitosan/SS microparticles with a smooth surface (41–48 µm) and high loading efficiency (69–75%). SS is released from the system through an in vitro process in a phosphate buffer at pH 7.4 and 37 °C over 24 h. As the SS concentration increases, the antioxidant properties and release content also increase.…”

“…After the initial burst, a sustained release was verified. After 24 h, these release levels increased to 45%, 55%, and 72% [ 98 ]. This enhancement in antioxidant properties is beneficial for wound healing because antioxidants can reduce oxidative stress in the wound area, thereby improving the healing process by protecting cells from damage.…”