2022
DOI: 10.1016/j.ijbiomac.2022.04.052
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Chitosan‑sodium alginate-collagen/gelatin three-dimensional edible scaffolds for building a structured model for cell cultured meat

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Cited by 55 publications
(23 citation statements)
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“…Similarly, porous plant protein scaffolds are feasible and relatively easy to produce in terms of technical complexity, as the main process steps are freeze‐drying and/or steam sterilization. The formation of a porous platform relies on crosslinks between plant‐based proteins, including physical (Wei et al, 2023; Xiang et al, 2022) and electrostatic interaction (Chen et al, 2023; Li et al, 2022). The simple principles of this method make it easier for industrial manufacturers and is free of animal‐derived materials making it more acceptable to consumers.…”
Section: Scalability and Feasibility Of Current Structuring Methodsmentioning
confidence: 99%
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“…Similarly, porous plant protein scaffolds are feasible and relatively easy to produce in terms of technical complexity, as the main process steps are freeze‐drying and/or steam sterilization. The formation of a porous platform relies on crosslinks between plant‐based proteins, including physical (Wei et al, 2023; Xiang et al, 2022) and electrostatic interaction (Chen et al, 2023; Li et al, 2022). The simple principles of this method make it easier for industrial manufacturers and is free of animal‐derived materials making it more acceptable to consumers.…”
Section: Scalability and Feasibility Of Current Structuring Methodsmentioning
confidence: 99%
“…Immortal cell lines minimize the need for continual animal biopsies. The C2C12 murine myoblast cell line has been extensively used as a model cell line in cell‐based meats to evaluate biomaterial performance and as a proof of concept (Andreassen et al, 2022; Choi et al, 2023; Furuhashi et al, 2021; Guan et al, 2023; Ikeda & Takeuchi, 2019; Kong et al, 2022; Kong & Huang, 2023; Li et al, 2022; Liu et al, 2022; Norris et al, 2022; Okamoto et al, 2022; Tzimorotas et al, 2023; Wei et al, 2023; Xiang et al, 2022). Cell‐based meats that are food‐safe and commercially available will be made from culinarily appropriate animal cells derived from animals such as cows, pigs, fish, and chickens.…”
Section: From Research Lab To Commercial Scalementioning
confidence: 99%
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“…Carboxymethylation improves the solubility of chitosan, such as Carboxymethyl Chitosan (Shariatinia, 2018; Islam et al ., 2020). Studies have shown successful application of chitosan‐sodium alginate‐collagen/gelatin scaffolds for porcine skeletal muscle differentiation (Li et al ., 2022) and the development of chitosan and collagen‐based edible hydrogel cell microcarriers for C2C12 (Zernov et al ., 2022).…”
Section: Edible Scaffolds and Microcarriersmentioning
confidence: 99%
“…The combination of the properties of sodium alginate and chitosan in one material made it possible to develop hemostatic agents [14] in the form of sponges, as well as scaffolds for neural tissue engineering [15]. In turn, the combination of the properties of collagen and chitosan, as well as gelatin and chitosan, contributed to the development of scaffolds for skin regeneration and matrices for cell cultivation in vitro, respectively [10,[16][17][18]. When developing new materials, combinations of polymers of synthetic and natural origin are widely used.…”
Section: Introductionmentioning
confidence: 99%