Chlamydia pneumoniae infection of brain cells: An in vitro study

Boelen, Ellen; Steinbusch, Harry W.M.; Ven, A.J.A.M. van der; Grauls, Gert; Bruggeman, Cathrien A.; Stassen, Frank R. M.

doi:10.1016/j.neurobiolaging.2006.02.014

Cited by 37 publications

(41 citation statements)

References 40 publications

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“…A deposition is induced in mice brain following intranasal infection with C. pneumoniae isolated from an AD brain, suggesting the infection involved in the initiation of AD (Little et al, 2004). In in vitro experiment, C. pneumoniae infection induced the elevation of pro-inflammatory cytokines in culture supernatant of mice astrocyte, including MCP-1, IL-6, TNF- (Boelen et al, 2007). The cell death was markedly increased when the neurons were putting into the culture supernatant of mice astrocyte infected with C. pneumoniae (Balin and Hudson, 2014;Boelen et al, 2007).…”

Section: Microbial Infection and Admentioning

confidence: 99%

“…In in vitro experiment, C. pneumoniae infection induced the elevation of pro-inflammatory cytokines in culture supernatant of mice astrocyte, including MCP-1, IL-6, TNF- (Boelen et al, 2007). The cell death was markedly increased when the neurons were putting into the culture supernatant of mice astrocyte infected with C. pneumoniae (Balin and Hudson, 2014;Boelen et al, 2007). The results show that C. pneumoniae infection may stimulate pro-inflammatory response and promote the production of a series of cytokines and chemokines through activating microglia and astrocytes, and then lead to neurodegeneration and play an important role in neuroinflammation and even AD pathology.…”

Section: Microbial Infection and Admentioning

confidence: 99%

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Alzheimer’s disease and gut microbiota

Wang

Jin

2016

Sci. China Life Sci.

300

188

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Section: Microbial Infection and Admentioning

confidence: 99%

Section: Microbial Infection and Admentioning

confidence: 99%

Alzheimer’s disease and gut microbiota

Wang

Jin

2016

Sci. China Life Sci.

300

188

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“…In fact, C. pneumoniae infects and replicates mainly within monocytes and macrophages, but also within lymphocytes and human brain endothelial cells (Stratton and Sriram, 2003), alters the permeability of BBB and promotes the migration of monocytes through human brain endothelial cells (MacIntyre et al, 2003;Stratton and Sriram, 2003;Stratton and Wheldon, 2006). C. pneumoniae also induces a productive infection in microglial cells, astrocytes and neurons (Dreses-Werringloer et al, 2006;Boelen et al, 2006;Ikejima et al, 2006), has been associated to some acute and monophasic neurological syndromes involving both central and peripheral nervous system (Stratton and Sriram, 2003;Stratton and Wheldon, 2006), and has been implicated in Alzheimer's disease and cerebrovascular diseases (Yucesan and Sriram, 2001;Stratton and Sriram, 2003). The infection hypothesis seems to reconcile these findings and make C. pneumoniae an attractive candidate for MS autoimmunity.…”

Section: Chlamydia Pneumoniae As Putative Candidate For Msmentioning

confidence: 99%

Under the Microscope: Focus on Chlamydia pneumoniae Infection and Multiple Sclerosis

Fainardi¹,

Castellazzi²,

Seraceni³

et al. 2008

CNR

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Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the Central Nervous System (CNS) of supposed autoimmune origin whose etiology still remains unknown. Epidemiological studies suggest that MS pathogenesis could be related to an infection superimposed on a predisposing genetic background. However, at present, no direct evidence for an infectious implication in MS autoimmunity exists. Recently, the potential role of Chlamydia pneumoniae as a causative agent of MS has received increasing attention. After the initial high rates reported for molecular and culture demonstration of C. pneumoniae in cerebrospinal fluid (CSF) of MS patients, the association between C. pneumoniae and MS was intensively investigated with controversial results. Seroepidemiological reports did not show any strong association between C. pneumoniae infection and the risk of MS. Isolation techniques failed to detect C. pneumoniae in CSF and brain tissue of MS patients. Polymerase chain reaction (PCR) evidence of C. pneumoniae in CSF and intrathecal synthesis of anti-C. pneumoniae IgG have been undetectable in MS patients or, if present, were not selectively associated with MS, but were shared by several inflammatory neurological conditions. Nevertheless, in a subgroup of MS patients, an association between PCR positivity for C. pneumoniae in CSF and disease activity was found. Intrathecal production of anti-C. pneumoniae high-affinity IgG predominated in MS progressive forms and metabolically active C. pneumoniae was identified in CSF. C. pneumoniae was recognized in CSF and brain tissue at immunohistochemical, molecular and ultrastructural levels. C. pneumoniae was also able to induce the animal model of MS. This growing body of data does not support a central role for C. pneumoniae as a candidate in MS pathogenesis, but suggests that, in a subset of MS patients, C. pneumoniae could induce a chronic persistent brain infection acting as a cofactor in the development of the disease. Thus, the actual involvement of C. pneumoniae in MS still remains to be elucidated.

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“…Symptoms can be mild with nonproductive persistent cough, malaise, and fever, but more-severe illness occurs when the lower respiratory tracts are affected, giving rise to acute bronchitis and pneumonia (3,10,14). Cardiovascular disease and neurological diseases are serious nonrespiratory symptoms associated with C. pneumoniae and M. pneumoniae infections (4,5,11,13,28).…”

mentioning

confidence: 99%

Simultaneous Detection ofChlamydophila pneumoniaeandMycoplasma pneumoniaeby Use of Molecular Beacons in a Duplex Real-Time PCR

Gullsby

Storm

2008

J Clin Microbiol

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A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumoniae and the ompA gene for C. pneumoniae, was compared with two conventional PCR assays targeting the 16S rRNA gene and the ompA gene. A total of 120 clinical throat and nasopharyngeal swab samples were tested. DNA extraction was performed using an alkali denaturation/neutralization method, and real-time amplification, detection, and data analysis were performed using a Rotor-Gene 2000 real-time rotary analyzer (Corbett Life Science, Sydney, Australia). Using conventional PCR as a reference in an analysis of 120 samples, 13 of 14 samples positive for C. pneumoniae were detected by the novel real-time PCR. In an analysis of M. pneumoniae, 22 samples were positive in the conventional PCR and the novel assay detected 24 positive samples. When using the conventional PCR as a reference, sensitivity and specificity were 93% and 100%, respectively, for C. pneumoniae and 100% and 98%, respectively, for M. pneumoniae. With an overall agreement of 98.8%, this suggests that performance of the new duplex real-time PCR is comparable to that of conventional PCR.

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Chlamydia pneumoniae infection of brain cells: An in vitro study

Cited by 37 publications

References 40 publications

Alzheimer’s disease and gut microbiota

Alzheimer’s disease and gut microbiota

Under the Microscope: Focus on Chlamydia pneumoniae Infection and Multiple Sclerosis

Simultaneous Detection ofChlamydophila pneumoniaeandMycoplasma pneumoniaeby Use of Molecular Beacons in a Duplex Real-Time PCR

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