Chlamydia pneumoniae infection of the central nervous system

Yücesan, Canan; Subramaniam, Sriram

doi:10.1097/00019052-200106000-00015

Cited by 58 publications

(54 citation statements)

References 31 publications

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“…Chlamydia trachomatis causes infant pneumonia, trachoma, and sexually transmitted diseases, including pelvic inflammatory diseases (2,4). Chlamydia pneumoniae cause pneumonia, bronchitis, and, more recently, was found to be associated with cardiovascular diseases, especially atherosclerosis (5,6) and, in some degree, with neurological diseases such as Alzheimer's disease (7,8). There is no vaccine for chlamydial diseases at the present time.…”

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confidence: 99%

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Bilenki

Wang

Yang

et al. 2006

The Journal of Immunology

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Chlamydial infections are serious public health concerns worldwide. In this study, we examined the role of dendritic cell (DC) subsets in inducing protective immunity against chlamydial infection using an adoptive transfer approach. We found that CD11c+CD8α+ (double-positive, DP) DC, compared with CD11c+CD8α− (single-positive, SP) DC isolated from infected mice, are more potent inducers of protective immunity. Specifically, mice pretreated with DPDC from infected mice, upon infection with Chlamydia trachomatis mouse pneumonitis (MoPn), experienced significantly less severe body weight loss and in vivo chlamydial growth. Analysis of MoPn-driven cytokine production by immune cells revealed that mice that were treated with DPDC produced significantly higher levels of Th1 (TNF-α, IFN-γ, and IL-12) but lower levels of Th2 (IL-4, IL-5, and IL-13)-related cytokines than the recipients of SPDC following infection challenge. Moreover, DPDC-treated mice displayed significantly higher levels of MoPn-specific IgG2a production and delayed-type hypersensitivity responses compared with SPDC-treated mice. Furthermore, DPDC isolated from infected mice produced higher amounts of IL-12 and IL-10 in vitro in comparison with SPDC. These data indicate that CD8α+ DC have a significantly higher capacity in inducing protective immunity compared with CD8α− DC, demonstrating the crucial role of DC1-like cells in eliciting protection against C. trachomatis infection

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confidence: 99%

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Bilenki

Wang

Yang

et al. 2006

The Journal of Immunology

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“…Until now, no antibiotics have proven capable of eradicating persistent chlamydial infections (21,22). An association between persistent C. pneumoniae and the development and progression of chronic degenerative diseases such as atherosclerosis, neurological disorders, and asthma has been suggested (14,27,34,37,38).…”

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confidence: 99%

Comparison of Quantitative and Semiquantitative Enzyme-Linked Immunosorbent Assays for Immunoglobulin G against Chlamydophila pneumoniae to a Microimmunofluorescence Test for Use with Patients with Respiratory Tract Infections

Hermann

Gueinzius

Oehme³

et al. 2004

J Clin Microbiol

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We previously reported a high degree of variation in the sensitivities of serodiagnostic kits for the detection of Chlamydophila pneumoniae in sera from healthy donors. Since a low predictive value of a test can impair its diagnostic value, we have extended our studies to samples from patients with pneumonia. We focused on the most promising enzyme-linked immunosorbent assays (ELISAs) (SeroCP and SeroCP Quant; Savyon) identified in our previous study and included a new ELISA (sELISA; Medac). The agreement between all ELISAs for immunoglobulin G (IgG) and a reference microimmunofluorescence (MIF) test for IgG (SeroFIA; Savyon) was >90% for a collective of 80 patients. The positive predictive values were all >93%. The negative predictive values ranged from 68 to 83%. False-negative results were obtained only for samples that had low titers in the MIF test. The correlation of the IgG antibody titers determined by the MIF and SeroCP Quant tests was high (r sp ‫؍‬ 0.9). Since the semiquantitative SeroCP and quantitative SeroCP Quant ELISAs achieved the highest sensitivities, they were evaluated further by using a second batch of sera from 50 patients with predominantly medium and low antibody titers in the MIF test and a control collection of sera from 80 children with negative MIF results. Again, the tests showed a high concordance with the MIF results (96%), and the antibody titers in the SeroCP Quant and MIF tests correlated well (r sp ‫؍‬ 0.8). The specificities determined with the negative sera were >99% for the SeroCP Quant test and 86% for the SeroCP test. These results show that ELISAs that are fast and objective deliver seroprevalence results, sensitivities, and specificities that are very similar to those of the MIF test.

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“…Infection with C. pneumoniae has been implicated in the pathogenesis of severe cardiovascular diseases, especially atherosclerosis (32); however, the role of this pathogen as etiologic agent has not yet been proven and is still controversial (4,13,23,31). Likewise, the possible involvement of C. pneumoniae in the pathogenesis of neurodegenerative diseases and lung cancer is intensely debated (18,43). Antibodies to this organism are spread worldwide in the adult population (17).…”

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confidence: 99%

Detection ofChlamydia pneumoniae-Specific Antibodies Binding to the VD2 and VD3 Regions of the Major Outer Membrane Protein

et al. 2003

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Although Chlamydia pneumoniae is an important human pathogen, the antigens eliciting a specific humoral immune response remain elusive. We scrutinized several recombinant chlamydial surface proteins for speciesspecific recognition by a panel of human sera previously tested for the presence of anti-C. pneumoniae and anti-C. trachomatis antibodies by microimmunofluorescence and enzyme-linked immunosorbent assay. The 15-kDa cysteine-rich protein (CrpA), porin-b (PorB), 9-kDa outer membrane protein (OMP3), 60-kDa outer membrane protein (OMP2), and four fragments of the major outer membrane protein (MOMP) representing each variable domain (VD) were overexpressed in Escherichia coli, affinity purified, and employed for Western blot analysis. None of the sera tested contained antibodies recognizing PorB and OMP3 of C. pneumoniae. Sera from C. pneumoniae-immune patients cross-reacted with OMP2 of C. trachomatis, and sera from C. trachomatisimmune patients cross-reacted with CrpA of C. pneumoniae, indicating that some of chlamydial surface molecules share antigenic epitopes. In contrast, the VD2, as well as the VD3, regions of the MOMP of C. pneumoniae were only recognized by C. pneumoniae-positive sera, suggesting the existence of species-specific epitopes. The identification of such epitopes of cell surface molecules provides new insights into C. pneumoniaespecific immune responses and may be of value for the improvement of C. pneumoniae-specific diagnostic assay systems based on defined recombinant antigens.

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Chlamydia pneumoniae infection of the central nervous system

Cited by 58 publications

References 31 publications

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Comparison of Quantitative and Semiquantitative Enzyme-Linked Immunosorbent Assays for Immunoglobulin G against Chlamydophila pneumoniae to a Microimmunofluorescence Test for Use with Patients with Respiratory Tract Infections

Detection ofChlamydia pneumoniae-Specific Antibodies Binding to the VD2 and VD3 Regions of the Major Outer Membrane Protein

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