Chlamydia-related bacteria in respiratory samples in Finland

Niemi, Suvi; Greub, Gilbert; Puolakkainen, Mirja

doi:10.1016/j.micinf.2011.04.012

Cited by 31 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rhabdochlamydia porcellionis," first detected in terrestrial isopods (25,26), and "Ca. Rhabdochlamydia crassificans," found in the cockroach Blatta orientalis (27) Since then, rhabdochlamydial DNA has also been detected in dwarf spiders (28), sea birds (29), and ticks (14,24) but also in human respiratory samples (11,17,30). However, it is unknown how Rhabdochlamydiaceae could be transmitted between animal species and to humans, but ticks could serve as vectors (23).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Hokynar

Vesterinen

Lilley

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

Chlamydia-like organisms (CLOs) are recently identified members of the Chlamydiales order. CLOs share intracellular lifestyles and biphasic developmental cycles, and they have been detected in environmental samples as well as in various hosts such as amoebae and arthropods. In this study, we screened bat feces for the presence of CLOs by molecular analysis. Using pan-Chlamydiales PCR targeting the 16S rRNA gene, Chlamydiales DNA was detected in 54% of the specimens. PCR amplification, sequencing, and phylogenetic analysis of the 16S rRNA and 23S rRNA genes were used to classify positive specimens and infer their phylogenetic relationships. Most sequences matched best with Rhabdochlamydia species or uncultured Chlamydia sequences identified in ticks. Another set of sequences matched best with sequences of the Chlamydia genus or uncultured Chlamydiales from snakes. To gain evidence of whether CLOs in bat feces are merely diet borne, we analyzed insects trapped from the same location where the bats foraged. Interestingly, the CLO sequences resembling Rhabdochlamydia spp. were detected in insect material as well, but the other set of CLO sequences was not, suggesting that this set might not originate from prey. Thus, bats represent another potential host for Chlamydiales and could harbor novel, previously unidentified members of this order. IMPORTANCE Several pathogenic viruses are known to colonize bats, and recent analyses indicate that bats are also reservoir hosts for bacterial genera. Chlamydialike organisms (CLOs) have been detected in several animal species. CLOs have high 16S rRNA sequence similarity to Chlamydiaceae and exhibit similar intracellular lifestyles and biphasic developmental cycles. Our study describes the frequent occurrence of CLO DNA in bat feces, suggesting an expanding host species spectrum for the Chlamydiales. As bats can acquire various infectious agents through their diet, prey insects were also studied. We identified CLO sequences in bats that matched best with sequences in prey insects but also CLO sequences not detected in prey insects. This suggests that a portion of CLO DNA present in bat feces is not prey borne. Furthermore, some sequences from bat droppings not originating from their diet might well represent novel, previously unidentified members of the Chlamydiales order.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Some of these CLOs are suggested to have human-pathogenic potential: Waddlia chondrophila is associated with adverse pregnancy outcomes (8), and Simkania negevensis and Rhabdochlamydia spp. are associated with respiratory infections (9)(10)(11).…”

mentioning

confidence: 99%

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Hokynar

Vesterinen

Lilley

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Indeed, using a specific quantitative PCR, Niemi et al could not identify any positive case of Simkania infection in 97 respiratory samples taken from adult patients with a suspected respiratory tract infection (Niemi et al, 2011). Similarly, only two samples from Swiss children with bronchiolitis were identified as positive for a Simkaniaceae infection by a validated pan-Chlamydiales quantitative PCR (Lienard et al, 2011a) and only one case of a co-infection with Simkania and C. psittaci was identified in a large German study investigating community acquired pneumonia (Dumke et al, 2015).…”

Section: Lessons From the Clinicmentioning

confidence: 99%

Simkania negevensis, an insight into the biology and clinical importance of a novel member of the Chlamydiales order

Vouga

Baud

Greub

2016

Critical Reviews in Microbiology

Self Cite

View full text Add to dashboard Cite

Simkania negevensis is a Chlamydia-related bacterium discovered in 1993 and represents the founding member of the Simkaniaceae family within the Chlamydiales order. As other Chlamydiales, it is an obligate intracellular bacterium characterized by a biphasic developmental cycle. Its similarities with the pathogenic Chlamydia trachomatis and Chlamydia pneumoniae make it an interesting bacterium. So far, little is known about its biology, but S. negevensis harbors various microbiological characteristics of interest, including a strong association of the Simkania-containing vacuole with the ER and the presence of an intron in the 23S rRNA encoding gene. Evidence of human exposition has been reported worldwide. However, there is a lack of robust clinical studies evaluating its implication in human diseases; current data suggest an association with pneumonia and bronchiolitis making S. negevensis a potential emerging pathogen. Owing to its fastidious growth requirements, the clinical relevance of S. negevensis is probably underestimated. In this review, we summarize the current knowledge on S. negevensis and explore future research challenges. ARTICLE HISTORY

show abstract

“…crassificans was first isolated from a cockroach and has also been recently detected by PCR in ticks (Ixodes ricinus) collected in Switzerland and in Algeria , suggesting that arthropods could act as widespread reservoirs and vectors of transmission for this species. The pathogenicity of Rhabdochlamydia has not been extensively studied yet but a few reports suggest a possible role in respiratory infections and in bovine abortion (Haider et al, 2008;Lamoth et al, 2009;2011;Wheelhouse et al, 2010;Niemi et al, 2011).…”

Section: Chlamydiales As Examplesmentioning

confidence: 99%

Importance of amoebae as a tool to isolate amoeba‐resisting microorganisms and for their ecology and evolution: the Chlamydia paradigm

Kebbi-Beghdadi

Greub

2014

Environ Microbiol Rep

Self Cite

View full text Add to dashboard Cite

Free-living amoebae are distributed worldwide and are frequently in contact with humans and animals. As cysts, they can survive in very harsh conditions and resist biocides and most disinfection procedures. Several microorganisms, called amoeba-resisting microorganisms (ARMs), have evolved to survive and multiply within these protozoa. Among them are many important pathogens, such as Legionella and Mycobacteria, and also several newly discovered Chlamydia-related bacteria, such as Parachlamydia acanthamoebae, Estrella lausannensis, Simkania negevensis or Waddlia chondrophila whose pathogenic role towards human or animal is strongly suspected. Amoebae represent an evolutionary crib for their resistant microorganisms since they can exchange genetic material with other ARMs and develop virulence traits that will be further used to infect other professional phagocytes. Moreover, amoebae constitute an ideal tool to isolate strict intracellular microorganisms from complex microbiota, since they will feed on other fast-growing bacteria, such as coliforms potentially present in the investigated samples. The paradigm that ARMs are likely resistant to macrophages, another phagocytic cell, and that they are likely virulent towards humans and animals is only partially true. Indeed, we provide examples of the Chlamydiales order that challenge this assumption and suggest that the ability to multiply in protozoa does not strictly correlate with pathogenicity and that we should rather use the ability to replicate in multiple and diverse eukaryotic cells as an indirect marker of virulence towards mammals. Thus, cell-culture-based microbial culturomics should be used in the future to try to discover new pathogenic bacterial species.

show abstract

Chlamydia-related bacteria in respiratory samples in Finland

Cited by 31 publications

References 22 publications

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Simkania negevensis, an insight into the biology and clinical importance of a novel member of the Chlamydiales order

Importance of amoebae as a tool to isolate amoeba‐resisting microorganisms and for their ecology and evolution: the Chlamydia paradigm

Contact Info

Product

Resources

About