Chlamydia trachomatis detected in human placenta.

Gencay, Mesut; Puolakkainen, Mirja; Wahlström, Torsten; Ämmälä, Pirkko; Mannonen, Laura; Vaheri, Antti; Koskiniemi, M.

doi:10.1136/jcp.50.10.852

Cited by 21 publications

(15 citation statements)

References 18 publications

(1 reference statement)

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“…These findings agree with previously published data, suggesting that Ct can be found in the lower respiratory tract of newborns and can lead to pneumonitis [12,15,21]. Evidence for the presence of Ct in the human placental tissue also exists [40][41][42].…”

Section: Discussionsupporting

confidence: 92%

Occurrence ofChlamydia trachomatisandChlamydia pneumoniaein paediatric respiratory infections

Webley¹,

Tilahun²,

Lay³

et al. 2008

Eur Respir J

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An emerging body of evidence suggests that half of asthma in both children and adults is associated with chronic lung infection. The aim of the present study was to determine the frequency of viable Chlamydia pneumoniae (Cp) and C. trachomatis (Ct) in the respiratory tracts of paediatric patients with chronic respiratory diseases.Bronchoalveolar lavage fluid (BALF) samples obtained from 182 children undergoing bronchoscopy for clinical reasons were assayed using PCR analysis, in vitro tissue culture and immunofluorescence staining for the presence of Cp and Ct.Chlamydia-specific DNA was detected by PCR in 124 (68%) out of 182 patients; 79 were positive for Cp, 77 positive for Ct and 32 for both organisms; 75 patients had cultivable Chlamydia. Ct DNA prevalence decreased, whereas Cp positivity generally increased with age. A total of 59 out of 128 asthma patients and 16 out of 54 nonasthmatics were Chlamydia culture positive. When the patients were divided into inflammatory versus noninflammatory airway disease, there were 69 (46%) out of 150 and six (18%) out of 32 BALF samples with cultivable Chlamydia, respectively.Viable Chlamydia pneumoniae and Chlamydia trachomatis occur frequently in children with chronic respiratory diseases and may be more prevalent in asthma patients. To the current authors' knowledge, this is the first report of viable Chlamydia trachomatis in the lungs of children.

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Section: Discussionsupporting

confidence: 92%

Occurrence ofChlamydia trachomatisandChlamydia pneumoniaein paediatric respiratory infections

Webley¹,

Tilahun²,

Lay³

et al. 2008

Eur Respir J

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“…One case report described a stillborn at 36 weeks of gestation in whose placenta C. trachomatis was identified [31]. In a Chinese study 59 specimens of chorionic villi were examined that had been collected from women attending an antenatal clinic for artificial abortion within the first trimester of pregnancy, of which three cases (5%) were found to be C. trachomatis positive [32].…”

Section: Discussionmentioning

confidence: 99%

Chlamydia trachomatis and placental inflammation in early preterm delivery

et al. 2011

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Chlamydiatrachomatis may infect the placenta and subsequently lead to preterm delivery. Our aim was to evaluate the relationship between the presence of Chlamydiatrachomatis and signs of placental inflammation in women who delivered at 32 weeks gestation or less. Setting: placental histology and clinical data were prospectively obtained from 304 women and newborns at the Erasmus MC-Sophia, Rotterdam, the Netherlands. C.trachomatis testing of placentas was done retrospectively using PCR. C.trachomatis was detected in 76 (25%) placentas. Histological evidence of placental inflammation was present in 123 (40%) placentas: in 41/76 (54%) placentas with C.trachomatis versus 82/228 (36%) placentas without C.trachomatis infection (OR 2.1, 95% CI 1.2–3.5). C.trachomatis infection correlated with the progression (P = 0.009) and intensity (P = 0.007) of materno-fetal placental inflammation. C.trachomatis DNA was frequently detected in the placenta of women with early preterm delivery, and was associated with histopathological signs of placental inflammation.

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“…Specific probes can be designed to locate organisms that cannot be cultured (Hayashi et al 1990, Komminoth & Werner 1997 or visualized by conventional methods, such as viruses (Lewis & Wells 1992), Chlamydiae (Campbell et al 1993) and Mycobacterium paratuberculosis (Hulten et al 2000). In addition to identifying the pathogens, ISH is an increasingly popular tool for locating pathogenic organisms in their target tissues (Gebhart et al 1994, Loy et al 1996, Gencay 1997, Gumus et al 1997, Jantos et al 1999, Chae et al 2002. The method is sufficiently sensitive that target nucleic acids can be detected in tissue sections using a light microscope and non-radioactively labeled probes (Brown 1998).…”

Section: Introductionmentioning

confidence: 99%

PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids

Hsieh¹,

Wu²,

Tung³

et al. 2007

Dis. Aquat. Org.

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Archived formalin-fixed, paraffin-embedded tissues from 28 diseased ornamental cichlid fish associated with visceral granulomas were examined by polymerase chain reaction (PCR) and in situ hybridization (ISH) for detection of Francisella-like bacteria (FLB). The 16S rDNA FLB-specific primer pair 180f/465r was used on naturally infected ornamental cichlids, resulting in 11 positive cases (39%). Using DNA probes, all 28 cases (100%) showed a positive reaction, and most labeled cells were observed in the visceral granulomas of infected individuals. FLB was detected in cells morphologically resembling epithelioid and endothelioid macrophages. ISH was more sensitive than PCR or routine histopathological examination, based on the examination of archived formalin-fixed, paraffin-embedded tissues in this study. Furthermore, this technique located a new fish pathogen, FLB, in ornamental cichlids. The causative agent was similar to the pathogen inducing systemic granulomas in tilapia. KEY WORDS: Francisella-like bacterium · FLB · PCR · In situ hybridization · Cichlid · Ornamental fish Resale or republication not permitted without written consent of the publisherDis Aquat Org 75: [29][30][31][32][33][34][35][36] 2007 Disease Diagnostic Center [STAADDC] and collected between 1998 and 2002) of ornamental fish with visceral granulomas for investigation of the causative agent. A DIG-labeled DNA probe specific for a novel FLB partial 16S rRNA gene (base pairs 180 to 485) was developed for localization of the agent in the infected tissues of ornamental fish. This is the first report concerning granulomatous disease in ornamental fish due to infection with FLB, and comparing the sensitivities of ISH, PCR and histopathological assays. MATERIALS AND METHODSSampling. Twenty-eight diseased cichlid ornamental fish, consisting of 11 species: firebird Aulonocara rubescens, elegans Pseudotropheus elegans, zebra Pseudotropheus zebra, Rhodes's chilo Chilotilapia rhoadesii, Malawi eyebiter Dimidiochromis compressiceps, brown discus Symphysodon aequifasciatus, deep-water hap Haplochromis electra, electric blue hap Sciaenochromis fryeri, blue-white labido Labidochromis caeruleus, Placidochromis milomo and Frontosa cichlid Cyphotilapia frontosa were used as samples for study by PCR and ISH (Table 1). The disease had been tentatively diagnosed as FLB infection by cytological and histopathological examinations. Samples from 3 FLBfree firebird A. rubescens, as diagnosed by PCR and ISH, were selected as negative controls.Tissue processing. Brain, eye, gill arch, kidney, spleen, liver, heart and gastrointestinal (GI) tract were removed and cut into small pieces of ~5 mm 3 , and placed into neutral buffered formalin solution (20:1 [v/v] ratio of fixative to tissue) for 16 h. The fixed samples were dehydrated through an alcohol series and embedded in paraffin according to standard laboratory procedures. Serial sections 4 µm thick were cut, floated on the surface of a water-bath and mounted on positively charged slides (Muto Pure Chemic...

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Chlamydia trachomatis detected in human placenta.

Cited by 21 publications

References 18 publications

Occurrence ofChlamydia trachomatisandChlamydia pneumoniaein paediatric respiratory infections

Occurrence ofChlamydia trachomatisandChlamydia pneumoniaein paediatric respiratory infections

Chlamydia trachomatis and placental inflammation in early preterm delivery

PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids

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