Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing

Mattmann, Prisca; Marti, H. R.; Borel, Nicole; Jelocnik, Martina; Albini, Sarah; Vogler, Barbara Renate

doi:10.1371/journal.pone.0226088

Cited by 39 publications

(40 citation statements)

References 51 publications

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“…This is in accordance with the findings of other studies performed in Switzerland. 20 21 C psittaci genotype B, which was found in one Eurasian collared dove, is the predominant genotype in the European pigeon population. 22 32 33 Genotype E, which was found in one fancy pigeon, infects a variety of avian species and is frequently found in pigeons worldwide.…”

Section: Discussionmentioning

confidence: 96%

Detection of Chlamydiaceae in Swiss wild birds sampled at a bird rehabilitation centre

Stalder

Marti

Borel

et al. 2020

Veterinary Record Open

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BackgroundAnnually, 800–1500 wild birds are admitted to the rehabilitation centre of the Swiss Ornithological Institute, Sempach, Lucerne, Switzerland. The workers of the centre come in close contact with the avian patients and might therefore be exposed to zoonotic agents shed by these birds, such as Chlamydia psittaci.MethodsIn the present study, 91 choanal, 91 cloacal and 267 faecal swabs from 339 wild birds of 42 species were investigated using a stepwise diagnostic approach.ResultsChlamydiaceae were detected in 0.9 per cent (0.3–2.6 per cent) of birds (n=3), all of them members of the Columbidae family. The Chlamydiaceae species of two of these birds (one Eurasian collared dove, one fancy pigeon) were identified as C psittaci types B and E by PCR and outer membrane protein A genotyping.ConclusionThe findings of the current study suggest that zoonotic transmission of Chlamydiaceae is very unlikely for songbird and waterfowl species tested herein, while pigeons might pose a risk to workers at rehabilitation centres.

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Section: Discussionmentioning

confidence: 96%

Detection of Chlamydiaceae in Swiss wild birds sampled at a bird rehabilitation centre

Stalder

Marti

Borel

et al. 2020

Veterinary Record Open

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“…Analysis and comparison of the above studies revealed that detection of C. psittaci from pharyngeal and fecal samples in pigeons with PCR technique is a proper method. Chlamydiosis in birds rose significantly in the Europe in recent years and their reports on chlamydia infections European countries including Switzerland ( 5 ), Germany ( 26 ), Belgium ( 27 ) and Spain ( 28 ). Prevalence of C. psittaci in pigeons confirmed about 16%, 29%, 40% and 52% respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Chlamydi avium had identified as a new member of this family which causes respiratory disease and diarrhea in pigeons and psittacine ( 3 ). Several European studies reported this bacterium in pigeons ( 3 – 5 ), although the zoonotic ability of this bacterium is still an enigma. Psittacosis in humans has similar symptoms to influenza which can lead to pneumonia and non-respiratory health problems such as endocarditis, myocarditis, meningitis and conjunctivitis ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Detection and identification of Chlamydia spp. from pigeons in Iran by nested PCR and sequencing

Golestani¹,

Khoshkhoo²,

Hosseini³

et al. 2020

IJM

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Background and Objectives: Chlamydia psittaci, an obligate intracellular, Gram-negative zoonotic pathogen, has eight serovars and nine genotypes isolated from avian species with higher frequency in parrots and pigeons. The aim of this study was to characterize Chlamydia spp. using nested PCR and sequencing. Materials and Methods: A total of 270 pharyngeal swab samples collected randomly from asymptomatic pigeons of 30 pigeon aviaries in Tehran province. DNA was extracted with specific kit and amplified by specific primers in the first PCR and outer membrane protein A (ompA) gene in the second PCR. Positive samples were sequenced and phylogenetic tree analyzed based on the ompA gene. Results: Records showed that 16 of 30 (53%) pigeon aviaries were positive for Chlamydia spp. Phylogenetic tree analysis revealed that 15 of 16 (93.7%) positive samples, belonged to C. psittaci genotype B whereas the other sample belonged to C. avium. C. psittaci detected in 50% of pigeon aviaries that is high rate in Tehran province. Conclusion: As C. psittaci is a zoonosis and life threaten pathogen for human being, these results indicate the significance of it detection in asymptomatic pigeons. Also, this is the first report of Chlamydia avium presence in Iranian pigeons which its zoonotic potential is still unknown.

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“…A negative control (aqua bidest.) and a positive control (synthesized oligonucleotide of the ompA gene of a C. psittaci field isolate "T0592/03, amazon parrot" (National Reference Centre for poultry and Rabbit Disease, University of Zurich); synthesized by Microsynth) were used in duplicates in each run [18].…”

Section: Psittaci Ompa Qpcrmentioning

confidence: 99%

“…Per sample, a reaction mix with a final volume of 50 µL containing 25 µL REDTaq ReadyMix (Merck KGaA, Darmstadt, Germany), 200 nM of the primers ompA F (CTU) and ompA rev [73], and 3 µL sample template with a DNA concentration of 25 ng/µL was prepared. Cycling conditions were 10 min at 95 [18]. The ompA sequences obtained in this study are available in GenBank under accession numbers MT450242-MT450276.…”

Section: Ompa Genotyping Pcrmentioning

confidence: 99%

Occurrence of Chlamydiaceae in Raptors and Crows in Switzerland

et al. 2020

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Bacteria of the family Chlamydiaceae are globally disseminated and able to infect many bird species. So far, 11 species of Chlamydia have been detected in wild birds, and several studies found chlamydial strains classified as genetically intermediate between Chlamydia (C.) psittaci and C.abortus. Recently, a group of these intermediate strains was shown to form a separate species, i.e., C.buteonis. In the present study, 1128 samples from 341 raptors of 16 bird species and 253 corvids representing six species were examined using a stepwise diagnostic approach. Chlamydiaceae DNA was detected in 23.7% of the corvids and 5.9% of the raptors. In corvids, the most frequently detected Chlamydia species was C.psittaci of outer membrane protein A (ompA) genotype 1V, which is known to have a host preference for corvids. The most frequently detected ompA genotype in raptors was M56. Furthermore, one of the raptors harbored C.psittaci 1V, and two others carried genotype A. C.buteonis was not detected in the bird population investigated, so it remains unknown whether this species occurs in Switzerland. The infection rate of Chlamydiaceae in corvids was high compared to rates reported in other wild bird species, but neither Chlamydiaceae-positive corvids nor raptors showed overt signs of disease. Since the Chlamydiaceae of both, raptors and crows were identified as C.psittaci and all C.psittaci genotypes are considered to be zoonotic, it can be suggested that raptors and crows pose a potential hazard to the health of their handlers.

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Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing

Cited by 39 publications

References 51 publications

Detection of Chlamydiaceae in Swiss wild birds sampled at a bird rehabilitation centre

Detection of Chlamydiaceae in Swiss wild birds sampled at a bird rehabilitation centre

Detection and identification of Chlamydia spp. from pigeons in Iran by nested PCR and sequencing

Occurrence of Chlamydiaceae in Raptors and Crows in Switzerland

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