Chloramphenicol: Topical Application in the Middle Ear

D'angelo, E.; Patterson, W. C.; Morrow, Rufus C.

doi:10.1001/archotol.1967.00760040684017

Cited by 21 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous ultrastructural studies of the RWM in several species [1][2][3][4][5][6][7] have shown that the RWM is composed of three layers: the epithelial layer lining the middle ear cavity, the mid dle layer containing fibroblasts and fiber components, and the inner layer facing the perilymph. It has been fully investigated by animal experiments that various sub stances such as horseradish peroxidase [8], radioactive isotopes [9,10], proteins [10], antibiotics [11][12][13][14], toxins [15] and ferritin [14] can pass into the scala tympani via the RWM. It is also well accepted that the RWM plays a role in releasing the perilymphatic pressure produced by the movement of the stapes and is an alternative route for sound energy to enter the cochlea.…”

Section: Introductionmentioning

confidence: 99%

Subepithelial Fiber Components of the Round Window Membrane of the Guinea Pig: An Ultrastructural and Immunohistochemical Study

Yoshihara

Kaname²,

Ishii³

et al. 1995

ORL

View full text Add to dashboard Cite

Subepithelial fiber components of the round window membrane (RWM) of the guinea pig were studied by the following methods: transmission electron microscopy (TEM), scanning electron microscopy (SEM) with NaOH treatment, high-voltage electron microscopy (HVEM) and immunohistochemical staining. SEM observation revealed intersecting collagen fibers and a vascular network in the middle layer. TEM observation showed that at the attachment region to the bone fïbroblasts, collagen fibers and elastic fibers were intermingled with each other, and mesenchymal cells (bone-lining cells) were scattered on the surface of the bone. The cytoplasmic processes of fibroblasts and bone-lining cells were connected by interdigitations. HVEM demonstrated that collagen fibers of the RWM were directly linked to the fibers of the bone matrix. Immunohistochemically, the middle layer was positive for antibody to type I collagen. Immunoreaction for fibronectin was also positive at the middle layer and most intense in the region attached to the bone. Thus, the RWM is securely attached to the bone by type I collagen fibers together with the bone-lining cells and fibroblasts.

show abstract