Chlorate and nitrate reduction pathways are separately induced in the perchlorate-respiring bacterium Dechlorosoma sp. KJ and the chlorate-respiring bacterium Pseudomonas sp. PDA

Xu, Jinying; Trimble, John J.; Steinberg, Lisa M.; Logan, Bruce E.

doi:10.1016/j.watres.2003.10.017

Cited by 82 publications

(74 citation statements)

References 27 publications

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“…There is at least one organism, Pseudomonas sp. strain PDA, that expresses chlorate-reducing capability under aerobic growth conditions (44). We also found low but significant activity of Clr in I. dechloratans after 20 h of exponential growth under aerobic conditions, showing that Clr is not completely inhibited during aerobic growth.…”

Section: Discussionsupporting

confidence: 48%

“…strain PDA is the only strain reported to constitutively express (per)chlorate-reducing capacity. Aerobically grown cultures of the strain do not show any lag period when they are transferred to chlorate-respiring growth conditions (44). The high oxygen sensitivity of microbial (per)chlorate reduction in combination with long lag phases after exposure to oxygen reduces the efficiency of chlorate or perchlorate removal.…”

mentioning

confidence: 93%

“…strain KJ (27). The ability to perform (per)chlorate reduction is restored when (per)chlorate is offered as the sole electron acceptor, but recovery phases extending from hours to several days have been observed (26,39,44). The CRB Pseudomonas sp.…”

mentioning

confidence: 99%

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Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

Lindqvist

Johansson

Nilsson

et al. 2012

Appl Environ Microbiol

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ABSTRACTThe ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in the presence of different terminal electron acceptors in the chlorate reducerIdeonella dechloratans. Enzyme activity assays and mRNA analyses by real-time quantitative reverse transcription (qRT)-PCR were performed on cell extracts from cells grown aerobically with and without chlorate and on cells grown anaerobically in the presence of chlorate. Our results showed that both chlorite dismutase and chlorate reductase are expressed during aerobic growth. However, transfer to anaerobic conditions with chlorate resulted in significantly enhanced enzyme activities and mRNA levels for both enzymes. Absence of oxygen was necessary for the induction to occur, since chlorate addition under aerobic conditions produced neither increased enzyme activities nor higher relative levels of mRNA. For chlorite dismutase, the observed increase in activity was on the same order of magnitude as the increase in the relative mRNA level, indicating gene regulation at the transcriptional level. However, chlorate reductase showed about 200 times higher enzyme activity in anaerobically induced cells, whereas the increase in mRNA was only about 10-fold, suggesting additional mechanisms influence the enzyme activity.

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Section: Discussionsupporting

confidence: 48%

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confidence: 93%

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Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

Lindqvist

Johansson

Nilsson

et al. 2012

Appl Environ Microbiol

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“…Although perchlorate reduction was originally thought to be catalyzed by nitrate reductase (17), recent studies using perchlorate-reducing bacteria Dechloromonas sp. strain KJ (39) and Dechlorosoma suillum strains PS (11) and perc1ace (15) have suggested the presence of two distinct inducible enzymatic systems for perchlorate and nitrate reduction. Nitrate-grown strain KJ showed much lower levels of perchlorate than of nitrate reductase activity, and perchlorate reduction was delayed after exposure to nitrate (39).…”

Section: Vol 71 2005 Microbial Reduction Of Perchlorate and Nitratementioning

confidence: 99%

“…strain KJ (39) and Dechlorosoma suillum strains PS (11) and perc1ace (15) have suggested the presence of two distinct inducible enzymatic systems for perchlorate and nitrate reduction. Nitrate-grown strain KJ showed much lower levels of perchlorate than of nitrate reductase activity, and perchlorate reduction was delayed after exposure to nitrate (39). In strain PS, the expression of chlorite The removal of nitrate from soil did not shorten the perchlorate lag period or enhance the rate of perchlorate removal as we expected.…”

Section: Vol 71 2005 Microbial Reduction Of Perchlorate and Nitratementioning

confidence: 99%

Reduction of Perchlorate and Nitrate by Microbial Communities in Vadose Soil

Nozawa-Inoue

Scow

Rolston

2005

Appl Environ Microbiol

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Perchlorate contamination is a concern because of the increasing frequency of its detection in soils and groundwater and its presumed inhibitory effect on human thyroid hormone production. Although significant perchlorate contamination occurs in the vadose (unsaturated) zone, little is known about perchlorate biodegradation potential by indigenous microorganisms in these soils. We measured the effects of electron donor (acetate and hydrogen) and nitrate addition on perchlorate reduction rates and microbial community composition in microcosm incubations of vadose soil. Acetate and hydrogen addition enhanced perchlorate reduction, and a longer lag period was observed for hydrogen (41 days) than for acetate (14 days). Initially, nitrate suppressed perchlorate reduction, but once perchlorate started to be degraded, the process was stimulated by nitrate. Changes in the bacterial community composition were observed in microcosms enriched with perchlorate and either acetate or hydrogen. Denaturing gradient gel electrophoresis analysis and partial sequencing of 16S rRNA genes recovered from these microcosms indicated that formerly reported perchloratereducing bacteria were present in the soil and that microbial community compositions were different between acetate-and hydrogen-amended microcosms. These results indicate that there is potential for perchlorate bioremediation by native microbial communities in vadose soil.

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O–O Bond Formation by a Heme Protein: The Unexpected Efficiency of Chlorite Dismutase

DuBois

2014

Molecular Water Oxidation Catalysis

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Chlorate and nitrate reduction pathways are separately induced in the perchlorate-respiring bacterium Dechlorosoma sp. KJ and the chlorate-respiring bacterium Pseudomonas sp. PDA

Cited by 82 publications

References 27 publications

Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

Reduction of Perchlorate and Nitrate by Microbial Communities in Vadose Soil

O–O Bond Formation by a Heme Protein: The Unexpected Efficiency of Chlorite Dismutase

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