Chlorella vulgaris cultivation in pilot-scale to treat real swine wastewater and mitigate carbon dioxide for sustainable biodiesel production by direct enzymatic transesterification

Xie, Dian; Ji, Xiaowei; Zhou, Youcai; Dai, Jingxuan; He, Yongjin; Sun, Han; Guo, Zheng; Yang, Yi; Zheng, Xing; Chen, Bilian

doi:10.1016/j.biortech.2022.126886

Cited by 48 publications

(11 citation statements)

References 45 publications

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“…In order to evaluate the effects of different CO 2 concentrations on Chlorella’s growth, we chose two Chlorella strains that have been widely used in biotechnological applications and that have previously been used in studies connected with different environmental problems [ 23 , 24 , 25 , 26 , 27 ]. These Chlorella strains were then cultivated at 25 °C and 100 μE m −2 s −1 in Arnon medium under different CO 2 concentrations (0, 1, 3 and 5%).…”

Section: Resultsmentioning

confidence: 99%

CO2 Levels Modulate Carbon Utilization, Energy Levels and Inositol Polyphosphate Profile in Chlorella

Morales-Pineda

Garcia-Gómez

Bedera-García

et al. 2022

Plants

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Microalgae have a growing recognition of generating biomass and capturing carbon in the form of CO2. The genus Chlorella has especially attracted scientists’ attention due to its versatility in algal mass cultivation systems and its potential in mitigating CO2. However, some aspects of how these green microorganisms respond to increasing concentrations of CO2 remain unclear. In this work, we analyzed Chlorella sorokiniana and Chlorella vulgaris cells under low and high CO2 levels. We monitored different processes related to carbon flux from photosynthetic capacity to carbon sinks. Our data indicate that high concentration of CO2 favors growth and photosynthetic capacity of the two Chlorella strains. Different metabolites related to the tricarboxylic acid cycle and ATP levels also increased under high CO2 concentrations in Chlorella sorokiniana, reaching up to two-fold compared to low CO2 conditions. The signaling molecules, inositol polyphosphates, that regulate photosynthetic capacity in green microalgae were also affected by the CO2 levels, showing a deep profile modification of the inositol polyphosphates that over-accumulated by up to 50% in high CO2 versus low CO2 conditions. InsP4 and InsP6 increased 3- and 0.8-fold, respectively, in Chlorella sorokiniana after being subjected to 5% CO2 condition. These data indicate that the availability of CO2 could control carbon flux from photosynthesis to carbon storage and impact cell signaling integration and energy levels in these green cells. The presented results support the importance of further investigating the connections between carbon assimilation and cell signaling by polyphosphate inositols in microalgae to optimize their biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

CO2 Levels Modulate Carbon Utilization, Energy Levels and Inositol Polyphosphate Profile in Chlorella

Morales-Pineda

Garcia-Gómez

Bedera-García

et al. 2022

Plants

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“…Using livestock wastewater for microalgal cultivation seems to be another alternative solution. C. vulgaris MBFJNU-1 in natural swine wastewater (RSW) with 3% CO 2 resulted in the highest microalgal biomass (478.5 mg/l/day) and lipid (9.1 mg/l/day) productivities [ 90 ]. The livestock waste compost medium with 2,000 mg/l COD provided an optimal nutrient concentration for Chlorella sp.…”

Section: Phycoremediation: Wastewater As a Nutrient Source For Cultiv...mentioning

confidence: 99%

Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future Perspectives

Yamaoka

2022

J. Microbiol. Biotechnol.

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Heavy reliance on fossil fuels has been associated with increased climate disasters. As an alternative, microalgae have been proposed as an effective agent for biomass production. Several advantages of microalgae include faster growth, usage of non-arable land, recovery of nutrients from wastewater, efficient CO 2 capture, and high amount of biomolecules that are valuable for humans. Microalgae Chlorella spp. are a large group of eukaryotic, photosynthetic, unicellular microorganisms with high adaptability to environmental variations. Over the past decades, Chlorella has been used for the large-scale production of biomass. In addition, Chlorella has been actively used in various food industries for improving human health because of its antioxidant, antidiabetic, and immunomodulatory functions. However, the major restrictions in microalgal biofuel technology are the cost-consuming cultivation, processing, and lipid extraction processes. Therefore, various trials have been performed to enhance the biomass productivity and the lipid contents of Chlorella cells. This study provides a comprehensive review of lipid enhancement strategies mainly published in the last five years and aimed at regulating carbon sources, nutrients, stresses, and expression of exogenous genes to improve biomass production and lipid synthesis.

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“…Microalgae can be used as a primary source of feed/food, biofuels, fertilizer, plant growth promoters, biopesticides, antibiotics and medicines Halim et al, , 2019Adetunji et al, 2022;Fernandes et al, 2022). As biological factories, microalgae can also purify wastewaters and fix CO 2 in a sustainable manner (Adetunji et al, 2022;Fernandes et al, 2022;Goswami et al, 2022;Xie et al, 2022). In addition, microalgae are a good source of plant-based proteins and high-value nutraceutical compounds that can promote human health (Miranda et al, 1998;Herrero et al, 2006;Rea et al, 2011;Schwenzfeier et al, 2011;Caporgno and Mathys, 2018;Olasehinde et al, 2019;Gill et al, 2020;Ashaolu et al, 2021).…”

Section: Introduction: Chromatographic Separation Of Microalgal Biopr...mentioning

confidence: 99%

Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae

et al. 2022

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Microalgae are potential sources for the sustainable production of valuable chemicals including polyphenols, pigments, and ω-3 PUFAs. However, successful exploitation of these high value compounds in the food, healthcare and pharmaceutical sectors depends greatly on their effective separation, identification, and analysis after recovery from the biomass. The findings of this review paper illustrated that chromatographic methods coupled to different types of detectors have been used as a crucial part of research on microalgal polyphenols, Omega-3 Polyunsaturated Fatty Acids (ω-3 PUFAs), and pigments production through identification, measurement, sample preparation, and purification practices. Therefore, it is important to provide a comprehensive review regarding the current research in the field. The basic operating principles, parametric optimisation and detection units of common (liquid chromatography and gas chromatography) and novel chromatographic techniques (counter current chromatography, expanded bed adsorption chromatography and supercritical fluid chromatography) used to separate, identify, and quantify polyphenols, PUFAs and pigments from microalgae matrices are comprehensively reviewed.

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Chlorella vulgaris cultivation in pilot-scale to treat real swine wastewater and mitigate carbon dioxide for sustainable biodiesel production by direct enzymatic transesterification

Cited by 48 publications

References 45 publications

CO2 Levels Modulate Carbon Utilization, Energy Levels and Inositol Polyphosphate Profile in Chlorella

CO2 Levels Modulate Carbon Utilization, Energy Levels and Inositol Polyphosphate Profile in Chlorella

Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future Perspectives

Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae

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