Chlorine-resistant bacteria (CRB) in the reverse osmosis system for wastewater reclamation: Isolation, identification and membrane fouling mechanisms

Luo, Li-Wei; Wu, Yin-Hu; Chen, Genqiang; Wang, Haobin; Wang, Yunlong; Tong, Xin; Bai, Yuan; Xu, Yuan; Zhang, Ziwei; Ikuno, Nozomu; Hu, Hong-Ying

doi:10.1016/j.watres.2021.117966

Cited by 21 publications

(20 citation statements)

References 42 publications

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“…Previously reported biofouling-related genera had signi cantly higher relative abundances in the "aggravated" group, namely, ClO 2 , NaClO, UV, and FES. For instance, Methylobacterium, which is a typical disinfection-resistant and biofouling-related bacteria genus 23,35 , was dominant in the DRB bio lm of FES and ClO 2 with a relative abundance of 54.8 ± 2.3% and 28.6 ± 1.7%, respectively. Sphingobium, a highly secretory genus 24,39 , was found to dominate the DRB bio lm under UV (30.0 ± 0.8%).…”

Section: Microbial Community Analysis Of Drb Bio Lm On Ro Membranesmentioning

confidence: 99%

“…8. The relative abundance of Methylobacterium (a typical disinfection-resistance and biofouling-related genus 23,35 ) was negatively correlated with the normalised ux of the fouled RO membrane (p < 0.05). The relative abundances of two kinds of high EPS-secreting bacteria, Microbacterium and Pseudomonas, were also negatively correlated with the normalised ux.…”

Section: Correlation Between Microbial Community Structure and Ro Mem...mentioning

confidence: 99%

“…Unlike bacterial cells, the extracellular polymeric substances (EPS) on RO membranes have a more direct relationship with ux drop 31,35 . Hence, the total amount of organics in the DRB bio lms was measured by DOM (Fig.…”

Section: Bacteria and Dissolved Organic Matters (Dom) In The Drb Bio Lmsmentioning

confidence: 99%

“…Considering that the ratio of EPS to bacteria could affect the biofouling degree 35 , this study calculated the ratio of DOM to HPC in the DRB bio lms (Fig. 4b).…”

Section: Bacteria and Dissolved Organic Matters (Dom) In The Drb Bio Lmsmentioning

confidence: 99%

“…The bio lms of NaClO and ClO 2 were not very thick. However, the high DOM/HPC ratio in the bio lm could narrow the water channels between bacterial cells 35 , leading to the highest ux drop in the RO membrane.…”

Section: Morphological Characteristics Of Drb Bio Lmsmentioning

confidence: 99%

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Evaluation of biofouling characteristics on reverse osmosis membranes of disinfection-residual-bacteria (DRB) after seven kinds of disinfection

Wang²,

Wang

et al. 2022

Preprint

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Biofouling is a critical defect of the reverse osmosis (RO) system. It has been reported that disinfection processes tend to select certain undesirable disinfection-residual bacteria (DRB), leading to severe long-term biofouling potential. To provide constructive guidance on biofouling prevention in RO systems, this study evaluated the biofouling characteristics of RO membranes of DRB after the application of five mature disinfection methods (NaClO, NH2Cl, ClO2, UV, and O3) and two novel disinfection methods (K2FeO4 and flow-through electrode system (FES)). After a 32-day biofilm cultivation on the RO membranes, the DRB biofilm of K2FeO4 and O3 caused a slight normalised flux drop (22.4 ± 2.4% and 23.9 ± 1.7%, respectively) of RO membrane compared with the control group (non-disinfected, ~ 27% normalised flux drop). Moreover, the biofouling degree of the NH2Cl-DRB biofilm was similar to that of the control group. The remaining disinfection types aggravated membrane biofouling. The biofouling behaviour of DRB showed no relationship with bacterial concentration or activity. The thickness and density of the biofilms as well as the organics/bacterial number ratio in the DRB biofilm, helped explain the difference in the fouling degree between each group. Moreover, microbial community analysis showed that the relative abundance of typical highly secretory and biofouling-related genera, such as Pseudomonas, Sphingomonas, Acinetobacter, Methylobacterium, Sphingobium, and Ralstonia, were the main reasons for the difference in biofouling degree. All types of disinfection effectively prevented pathogen reproduction in the DRB biofilm. However, the relative abundance of (opportunistic) pathogens increased significantly after NaClO and ClO2 disinfection.

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Section: Microbial Community Analysis Of Drb Bio Lm On Ro Membranesmentioning

confidence: 99%

Section: Correlation Between Microbial Community Structure and Ro Mem...mentioning

confidence: 99%

Section: Bacteria and Dissolved Organic Matters (Dom) In The Drb Bio Lmsmentioning

confidence: 99%

“…Considering that the ratio of EPS to bacteria could affect the biofouling degree 35 , this study calculated the ratio of DOM to HPC in the DRB bio lms (Fig. 4b).…”

Section: Bacteria and Dissolved Organic Matters (Dom) In The Drb Bio Lmsmentioning

confidence: 99%

Section: Morphological Characteristics Of Drb Bio Lmsmentioning

confidence: 99%

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