Chlorite reactivity with myoglobin: Analogy with peroxide and nitrite chemistry?

Bischin, Cristina; Moţ, Augustin C.; Ştefancu, Andrei; Leopold, Nicolae; Hathazi, Denisa; Damian, Grigore; Silaghi-Dumitrescu, Radu

doi:10.1016/j.jinorgbio.2017.04.017

Journal of Inorganic Biochemistry

2017

DOI: 10.1016/j.jinorgbio.2017.04.017

|View full text |Cite

Chlorite reactivity with myoglobin: Analogy with peroxide and nitrite chemistry?

Cristina Bischin

Augustin C. Moţ

Andrei Ştefancu

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Preprint1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 51 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

The chlorite adduct of aquacobalamin: contrast with chlorite dismutase

Lehene,

Zagrean-Tuza,

Iancu

et al. 2024

Preprint

View full text Add to dashboard Cite

In the reaction of aquacobalamin (aquaCbl) with chlorite, a stable species is detected and assigned as a Co(III)-chlorite complex, Co(III)-OClO−. Its UV-vis spectrum is almost identical to that of aquaCbl, except for some minor differences at ~ 430 nm; cyanide can eliminate and prevent these changes. The 1H-NMR spectra reveal strong influences of chlorite on the B2 and B4 protons of the cobalt-bound dimethyl benzimidazole ligand. Together, the UV-vis and NMR titrations suggest a Kd of 10 mM or higher for chlorite on Cbl. Resonance Raman spectra reveal minor changes in the spectrum of aquaCbl to chlorite – as well as a disappearance of the free chlorite signals, consistent with Cbl-chlorite complex formation. Corroboration for these interpretations is also offered from mass spectrometry and DFT calculations. This Co(III)-OClO− complex would be a stable analogue of the first reaction intermediate in the catalytic cycle of chlorite dismutase, or in the reaction of chlorite with a number of other heme proteins. The differences in reactivity between Co(III) cobalamin and Fe(III) heme towards chlorite are analyzed and rationalized, leading to a reconciliation of experimental and computational data for the latter.

show abstract

The chlorite adduct of aquacobalamin: contrast with chlorite dismutase

Lehene,

Zagrean-Tuza,

Iancu

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Chlorite reactivity with myoglobin: Analogy with peroxide and nitrite chemistry?

Cited by 1 publication

References 51 publications

The chlorite adduct of aquacobalamin: contrast with chlorite dismutase

The chlorite adduct of aquacobalamin: contrast with chlorite dismutase

Contact Info

Product

Resources

About