Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences.

Nakatsu, Cindy H.; Ng, James; Singh, R. K.; Straus, Neil A.; Wyndham, Campbell

doi:10.1073/pnas.88.19.8312

Cited by 125 publications

(126 citation statements)

References 26 publications

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“…These transposons include both class I composite elements, in which catabolic genes are flanked by IS elements, and class II transposons that are all ancestrally related to the Tn3 family of transposons and are characterized by short (fewer than 50 bp) IRs and the involvement of a transposase (TnpA) and a resolvase (TnpR). However, the detailed structures of these catabolic transposons are extremely varied, and composite elements with features of both class I and class II are also known (22). The evolutionary origins of the opd gene and of the gene cluster that we describe in this paper are presently unknown.…”

Section: Vol 69 2003mentioning

confidence: 98%

Transposon-Like Organization of the Plasmid-Borne Organophosphate Degradation ( opd ) Gene Cluster Found in Flavobacterium sp

Siddavattam

Syed

Manavathi

et al. 2003

Appl Environ Microbiol

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Several bacterial strains that can use organophosphate pesticides as a source of carbon have been isolated from soil samples collected from diverse geographical regions. All these organisms synthesize an enzyme called parathion hydrolase, and in each case the enzyme is encoded by a gene (opd) located on a large indigenous plasmid. These plasmids show considerable genetic diversity, but the region containing the opd gene is highly conserved. Two opd plasmids, pPDL2 from Flavobacterium sp. and pCMS1 from Pseudomonas diminuta, are well characterized, and in each of them a region of about 5.1 kb containing the opd gene shows an identical restriction pattern. We now report the complete sequence of the conserved region of plasmid pPDL2. The opd gene is flanked upstream by an insertion sequence, ISFlsp1, that is a member of the IS21 family, and downstream by a Tn3-like element encoding a transposase and a resolvase. Adjacent to opd but transcribed in the opposite direction is an open reading frame (orf243) with the potential to encode an aromatic hydrolase somewhat similar to Pseudomonas putida TodF. We have shown that orf243 encodes a polypeptide of 27 kDa, which plays a role in the degradation of p-nitrophenol and is likely to act in concert with opd in the degradation of parathion. The linkage of opd and orf243, the organization of the genes flanking opd, and the wide geographical distribution of these genes suggest that this DNA sequence may constitute a complex catabolic transposon.

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Section: Vol 69 2003mentioning

confidence: 98%

Transposon-Like Organization of the Plasmid-Borne Organophosphate Degradation ( opd ) Gene Cluster Found in Flavobacterium sp

Siddavattam

Syed

Manavathi

et al. 2003

Appl Environ Microbiol

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“…Only three transposases were definitely outside the aggregate of the above clusters even when only the more conserved COOH-terminal part of the transposase protein were taken into account. These are a solitary transposase of IS2071 (Nakatsu et al, 1991) and a pair of closely related transposases of Tn.5041 and Tn46.52 (Fig. 3 ) .…”

Section: Tn5041 Belongs To the Tn3 Familymentioning

confidence: 99%

Tn5041: a chimeric mercury resistance transposon closely related to the toluene degradative transposon Tn4651

et al. 1997

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This paper reports the discovery and characterization of Tn5041, a novel-type transposon vehicle for dissemination of mercury resistance in natural bacterial populations. Tn5041 (14876 bp), identified in a Pseudomonas strain from a mercury mine, is a Tn3 family mercury resistance transposon far outside the Tn21 subgroup. As in other Tn3 family transposons, Tn5041 duplicates 5 bp of the target sequence following insertion. Tn5041 apparently acquired its mer operon as a single-ended relic of a transposon belonging to the classical mercury resistance transposons of the Tn27 subgroup. The putative transposase and the 47 bp terminal inverted repeats of Tn5041 are closely related to those of the toluene degradative transposon Tn4651 and fall into a distinct subgroup on the fringe of the Tn3 family. The amino acid sequence of the putative resolvase of Tn5041 resembles site-specif ic recombinases of the integrase family. Besides the mer operon and putative transposition genes, Tn5041 contains a 4 kb region that accommodates a number of apparently defective genes and mobile elements.

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“…The involvement of Tn5271-like elements in chlorobenzoate metabolism in these isolates was supported by analysing spontaneous deletion derivatives that showed a Cba-phenotype. The loss of the cbnAB locus was determined by hybridization and restriction mapping of the residual IS2072 element (Nakatsu et al 1991). The ability of type strains to act as recipients of pBRC60 in laboratory matings was determined using a Tn5 labelled derivative of this plasmid (pBRC40:Tn5) as described (Fulthorpe & Wyndham 1991).…”

Section: Dna Mnnipitlntionsmentioning

confidence: 99%

“…1; Nakatsu & Wyndham 1993). Following transformation into E. coli DH5a as described (Nakatsu et al 1991), plasmids were mobilized into recipients via triparental matings with E. coli HBlOl (pRK2013) and transconjugants were selected on 20 &mL tetracycline, 12-mM succinate, medium A. Strains used a s recipients are listed in Table 1.…”

Section: Dna Mnnipitlntionsmentioning

confidence: 99%

“…One strategy to investigate the impact of gene transfer on microbial fitness is to track the distribution of genes for the biodegradation of environmental contaminants (catabolic genes) under various selective conditions (Fulthorpe Q Wyndham 1992;Wyndham et al 1994a). We have been particularly interested in the transfer of genes involved in the initial hydroxylation of chlorinated aromatic pollutants (Nakatsu et al 1991;Nakatsu & Wyndham 1993; Nakatsu et al 1995). Because catabolic genes are often associated with conjugative plasmids and in some cases with transposons, they are ideal for investigating the influence of genetic mobility on fitness (Sayler et al 1990;Wyndham et al 1994b).…”

Section: Introductionmentioning

confidence: 99%

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The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

et al. 1995

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Horizontal gene transfer in the Bacteria has been demonstrated to occur under natural conditions. The ecological impact of gene transfer events depends on the new genetic material being expressed in recipient organisms, and on natural selection processes operating on these recipients. The phylogenetic distribution of cbaAB genes for chlorobenzoate 3,4-(4,5)-dioxygenase, which are carried within Tn5271 on the IncPP plasmid pBRC60, was investigated using isolates from freshwater microcosms and from the Niagara River watershed. The latter included isolates from surface water, groundwater and bioremediation reactor samples. The cbaAB genes have become integrated, through interspecific transfer, primarily into species of the p Proteobacteria (W48 isolates). Only four isolates, identified as Pseudomonas j7uorescens (3/48) and Xanthomonas maltophilia (l/48), belonged to the r Proteobacteria, despite the observation that pBRC60 was capable of mobilizing these genes into a wide range of j3 and r Proteobacteria in the laboratory. The natural host range correlated with the distribution of the meta-ring-fission pathway for metabolism of protocatechuates formed when the cbaAB genes were expressed (45/48 isolates). We proposed the hypothesis that natural selection has favoured recipients that successfully integrate the activity of the transferred dioxygenase with the conserved meta ring-fission pathway. The hypothesis was tested by transfemng a pIasmid construct containing the cbaAB genes into type strains representative of the j3 and r Proteobacteria. The concept of applying mobile catabolic genes to probe the phylogenetic distribution of compatible degradative pathways is discussed.

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Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences.

Abstract: The structure of a transposon specifying the biodegradation of chlorobenzoate contaminants is described. Ti5271 is a 17-kilobase (kb)

Cited by 125 publications

References 26 publications

Transposon-Like Organization of the Plasmid-Borne Organophosphate Degradation ( opd ) Gene Cluster Found in Flavobacterium sp

Transposon-Like Organization of the Plasmid-Borne Organophosphate Degradation ( opd ) Gene Cluster Found in Flavobacterium sp

Tn5041: a chimeric mercury resistance transposon closely related to the toluene degradative transposon Tn4651

The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

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