Chloroform

Holbrook, Michael T.

doi:10.1002/0471238961.0308121508151202.a01

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…In this research work, the LCI gathers solvent, antisolvent, energy to evaporate the solvent (enthalpy) and PHB. DMC enthalpy (0.194 kWh/kg) was reported by Breil et al [ 60 ] and chloroform enthalpy (0.069 kWh/kg) by Holbrook [ 61 ]. This energy to vaporize the solvent was supposed to be part of the system as a low-voltage power source.…”

Section: Methodsmentioning

confidence: 89%

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Mongili

Azim

Garofalo

et al. 2021

Biotechnol Biofuels

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Background Plastic plays a crucial role in everyday life of human living, nevertheless it represents an undeniable source of land and water pollution. Polyhydroxybutyrate (PHB) is a bio-based and biodegradable polyester, which can be naturally produced by microorganisms capable of converting and accumulating carbon as intracellular granules. Hence, PHB-producing strains stand out as an alternative source to fossil-derived counterparts. However, the extraction strategy affects the recovery efficiency and the quality of PHB. In this study, PHB was produced by a genetically modified Escherichia coli strain and successively extracted using dimethyl carbonate (DMC) and ethanol as alternative solvent and polishing agent to chloroform and hexane. Eventually, a Life Cycle Assessment (LCA) study was performed for evaluating the environmental and health impact of using DMC. Results Extraction yield and purity of PHB obtained via DMC, were quantified, and compared with those obtained via chloroform-based extraction. PHB yield values from DMC-based extraction were similar to or higher than those achieved by using chloroform (≥ 67%). To optimize the performance of extraction via DMC, different experimental conditions were tested, varying the biomass state (dry or wet) and the mixing time, in presence or in absence of a paper filter. Among 60, 90, 120 min, the mid-value allowed to achieve high extraction yield, both for dry and wet biomass. Physical and molecular dependence on the biomass state and solvent/antisolvent choice was established. The comparative LCA analysis promoted the application of DMC/ethanol rather than chloroform/hexane, as the best choice in terms of health prevention. However, an elevated impact score was achieved by DMC in the environmental-like categories in contrast with a minor contribution by its counterpart. Conclusion The multifaceted exploration of DMC-based PHB extraction herein reported extends the knowledge of the variables affecting PHB purification process. This work offers novel and valuable insights into PHB extraction process, including environmental aspects not discussed so far. The findings of our research question the DMC as a green solvent, though also the choice of the antisolvent can influence the impact on the examined categories.

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Section: Methodsmentioning

confidence: 89%

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Mongili

Azim

Garofalo

et al. 2021

Biotechnol Biofuels

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Vitamin B₁₂ as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter

Phillips,

Picott,

Kümmel

et al. 2024

MicrobiologyOpen

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Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and dechlorination rates and extent. We investigate the role of cobamide type on carbon and chlorine isotopic signals observed during reductive dechlorination of chloroform by the RDase CfrA. Microcosm experiments with two subcultures of a Dehalobacter‐containing culture expressing CfrA—one with exogenous cobamide (Vitamin B12, B12+) and one without (to drive native cobamide production)—resulted in a markedly smaller carbon isotope enrichment factor (εC, bulk) for B12− (−22.1 ± 1.9‰) compared to B12+ (−26.8 ± 3.2‰). Both cultures exhibited significant chlorine isotope fractionation, and although a lower εCl, bulk was observed for B12− (−6.17 ± 0.72‰) compared to B12+ (−6.86 ± 0.77‰) cultures, these values are not statistically different. Importantly, dual‐isotope plots produced identical slopes of ΛCl/C (ΛCl/C, B12+ = 3.41 ± 0.15, ΛCl/C, B12− = 3.39 ± 0.15), suggesting the same reaction mechanism is involved in both experiments, independent of the lower cobamide bases. A nonisotopically fractionating masking effect may explain the smaller fractionations observed for the B12− containing culture.

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Chloroform

Cited by 2 publications

References 12 publications

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Vitamin B₁₂ as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter

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Chloroform

Cited by 2 publications

References 12 publications

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

Vitamin B12 as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter

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Vitamin B₁₂ as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter