Chlorogenic Acid Decreases Malignant Characteristics of Hepatocellular Carcinoma Cells by Inhibiting DNMT1 Expression

Liu, Yao; Feng, Ying; Li, Yuxin; Hu, Ying; Zhang, Qun; Huang, Yunyi; Shi, Ke; Ran, Chongping; Hou, Jie; Zhou, Guiqin; Wang, Xianbo

doi:10.3389/fphar.2020.00867

Cited by 35 publications

(23 citation statements)

References 35 publications

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“…Likewise, dose-dependent inhibition of MDA-MB-231 breast cancer cells’ migration capabilities was observed with 50 µM and 100 µM of CA (wound area at 24 h was 9% and 6%, respectively) [ 32 ]. For CGA, it was observed the inhibition of liver cancer HepG2 cells migration at 48 h with 1000 µM [ 38 ], which is higher than the effect of the same compound on A549 lung cancer cells [ 39 , 40 ]. Likewise, in endothelial-derived cell models, e.g., HUVEC cells, it has been observed that cell motility induction under hypoxic conditions could be reversed by treatments with CGA at 10 µM for 24 h [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer

et al. 2021

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Colorectal cancer is one of the leading death-related diseases worldwide, usually induced by a multifactorial and complex process, including genetic and epigenetic abnormalities and the impact of diet and lifestyle. In the present study, we evaluated the biological impact of two of the main coffee polyphenols, chlorogenic acid (CGA) and caffeic acid (CA), as well as two polyphenol-rich coffee extracts (green coffee extract and toasted coffee Extract) against SW480 and SW620 colorectal cancer cells. First, the total phenolic content and the antioxidant capability of the extracts were determined. Then, cytotoxicity was evaluated by MTT and SBR. Finally, a wound healing assay was performed to determine the impact on the cell migration process. The results showed a cytotoxic effect of all treatments in a time and dose-dependent manner, which decreased the viability in both cell lines at 24 h and 48 h; likewise, the migration capability of cells decreased with low doses of treatments. These results suggest the potential of coffee to modulate biological mechanisms involved in colorectal cancer development; however, more studies are required to understand the mechanistic insights of these observations.

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Section: Discussionmentioning

confidence: 99%

Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer

et al. 2021

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“… [142] HepG2 human hepatocarcinoma cell In-vitro Inhibits the cellular proliferation, colony formation, invasion, and metastasis, MMP-2 and MMP-9 expressions, up-regulates p53 and p21 activity, and inactivates ERK1/2. [143] HepG2 human hepatocarcinoma cell In-vitro Promotes nuclear translocation of Nrf2, ARE reporter gene activity, and downstream antioxidant proteins (involving sestrin2, hemeoxygenase-1, glutamate cysteine ligase and NAD(P)H quinone oxidoreductase-1) [144] 5 Blood cancer Human U937 leukemia cell In-vitro Activates apoptosis via increasing ROS production, expression of caspase-3, 7, 8, 9, and decreasing the mitochondrial membrane potential (ΔΨm). [145] Human HL-60 leukemia cell In-vitro Increases the level of apoptosis in a dose-dependent manner, and halts G0/G1 phase cell cycle and proliferation.…”

Section: Potential Utilization Of Cga For the Therapeutics Of Cancermentioning

confidence: 99%

“…It was reported that the addition of CGA potentiates regorafenib actions in human hepatocellular carcinoma (HCC) cells [140] . CGA also promotes 5-fluorouracil effect in HCC cells via attenuating the extracellular signal regulated kinases (ERKs) [141] , exerts positive inhibitory effects in HCC (in vitro and in vivo) cells [142] , and reduced malignant attributes of HCC cells via suppressing DNMT1 expression [143] . Additionally, CGA enhanced the oxidative stress-mediated apoptosis via activating the nuclear factor erythroid 2-related factor 2 (Nrf2) in hepatocytes [144] .…”

Section: Chlorogenic Acid For the Therapeutics Of Liver Cancermentioning

confidence: 99%

Chlorogenic acid: Potential source of natural drugs for the therapeutics of fibrosis and cancer

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Lü

Zhang

et al. 2022

Translational Oncology

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Fibrosis and cancer is described by some epidemiological studies as chronic stages of different disease conditions typically characterized by uncontrolled accumulation of extra-cellular matrix (ECM), thereby leading to inflammation of tissues and organ (lungs, heart, liver and kidney) dysfunction. It is highly prevalent, and contributes to increased mortality rate worldwide. Currently, the therapeutical approaches involving selected medications (bemcentinib, pirfenidone and nintedanib) obtained synthetically, and used in clinical practices for fibrosis and cancer management and treatment has shown to be unsatisfactorily, especially during progressive stages of the disease. With regards to finding a more potent, effective, and promising curative for fibrosis and cancer, there is need for continuous experimental studies universally. However, phytochemical constituents’ particularly phenolic compounds [Chlorogenic acid (CGA)] obtained from coffee, and coffee beans have been predominantly utilized in experimental studies, due to its multiple pharmacological properties against various disease forms. Considering its natural source alongside minimal toxicity level, CGA, a major precursor of coffee have gained considerable attention nowadays from researchers worldwide, owing to its wide, efficacious and beneficial action against fibrosis and cancer. Interestingly, the safety of CGA has been proven. Furthermore, numerous experimental studies have also deduced massive remarkable outcomes in the use of CGA clinically, as a potential drug candidate against treatment of fibrosis and cancer. In the course of this review article, we systematically discussed the beneficial contributions of CGA with regards to its source, absorption, metabolism, mechanistic effects, and molecular mechanisms against different fibrosis and cancer categorization, which might be a prospective remedy in the future. Moreover, we also highlighted CGA (in vitro and in vivo analytical studies) defensive effects against various disorders.

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“…Increased levels or alterations in the function of DNMT-1 are associated with the inactivation of tumor suppressor genes. Liu et al [ 131 ] showed that CGA inhibited the proliferation, colony formation, invasion, and metastasis of HepG2 cells both in vitro and in vivo by down-regulating DNMT-1 protein expression, which enhanced p53 and p21 activity, and resulting in a significant reduction in cell proliferation and metastasis. Moreover, CGA inactivated ERK1/2 and reduced MMP-2 and MMP-9 expression in HepG2 cells.…”

Section: Anti-cancer Effects Of Coffeementioning

confidence: 99%

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

et al. 2020

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Tea and coffee are consumed worldwide and epidemiological and clinical studies have shown their health beneficial effects, including anti-cancer effects. Epigallocatechin gallate (EGCG) and chlorogenic acid (CGA) are the major components of green tea polyphenols and coffee polyphenols, respectively, and believed to be responsible for most of these effects. Although a large number of cell-based and animal experiments have provided convincing evidence to support the anti-cancer effects of green tea, coffee, EGCG, and CGA, human studies are still controversial and some studies have suggested even an increased risk for certain types of cancers such as esophageal and gynecological cancers with green tea consumption and bladder and lung cancers with coffee consumption. The reason for these inconsistent results may have been arisen from various confounding factors. Cell-based and animal studies have proposed several mechanisms whereby EGCG and CGA exert their anti-cancer effects. These components appear to share the common mechanisms, among which one related to reactive oxygen species is perhaps the most attractive. Meanwhile, EGCG and CGA have also different target molecules which might explain the site-specific differences of anti-cancer effects found in human studies. Further studies will be necessary to clarify what is the mechanism to cause such differences between green tea and coffee.

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Chlorogenic Acid Decreases Malignant Characteristics of Hepatocellular Carcinoma Cells by Inhibiting DNMT1 Expression

Cited by 35 publications

References 35 publications

Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer

Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer

Chlorogenic acid: Potential source of natural drugs for the therapeutics of fibrosis and cancer

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

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