Chlorogenic Acid Enhances Doxorubicin-Mediated Cytotoxic Effect in Osteosarcoma Cells

Salzillo, Alessia; Ragone, Angela; Spina, Annamaria; Naviglio, Silvio; Sapio, Luigi

doi:10.3390/ijms22168586

Cited by 26 publications

(15 citation statements)

References 65 publications

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“…Furthermore, flow cytometry analysis reported that the dose-dependent manners of ononin dramatically enhanced the apoptotic rate in human osteosarcoma cells and MG-63-xenograft mice. This study was consistent with an earlier report that chlorogenic acid, a polyphenol, induced significantly apoptosis and cell death in MG-63 and U2OS cells [28]. In this present study, we compared the effectiveness of DOX on osteosarcoma cells with that of ononin, as a positive control.…”

Section: Discussionsupporting

confidence: 92%

Anti-Invasive and Anti-Migratory Effects of Ononin on Human Osteosarcoma Cells by Limiting the MMP2/9 and EGFR-Erk1/2 Pathway

Gong

Ganesan

Xiong

et al. 2023

Cancers

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Osteosarcoma is a common malignancy of the bone. Due to its high metastatic properties, osteosarcoma becomes the leading cause of cancer death worldwide. Ononin is an isoflavone glycoside known to have various pharmacological properties, including antioxidant and anti-inflammatory activities. In the present study, we aimed to investigate the efficacy of ononin on osteosarcoma cell migration, invasion, and the underlying mechanisms. The in vitro anti-tumorigenic and anti-migratory properties of ononin were determined by MTT, colony formation, invasion, and migration in MG-63 and U2OS osteosarcoma cell lines. The results were compared with the standard chemotherapeutic drug, doxorubicin (DOX), as a positive control. The dose-dependent manners of ononin treatment increased the expression of apoptosis and inhibition of cell proliferation through the EGFR-Erk1/2 signaling pathways. Additionally, ononin significantly inhibited the invasion and migration of human osteosarcoma cells. For consistency, we used the MG-63-xenograft mice model to confirm the in vivo anti-tumorigenic and anti-migratory efficacy of ononin by inhibiting the protein expressions of EGFR-Erk1/2 and MMP2/9. According to the histological study, ononin had no adverse effect on the liver and kidney. Overall, our findings suggested that ononin could be a potentially effective agent against the development and metastasis of osteosarcoma.

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Section: Discussionsupporting

confidence: 92%

Anti-Invasive and Anti-Migratory Effects of Ononin on Human Osteosarcoma Cells by Limiting the MMP2/9 and EGFR-Erk1/2 Pathway

Gong

Ganesan

Xiong

et al. 2023

Cancers

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“…This can be supported by the unique mechanisms observed for these compounds and the outcomes of several clinical trial investigations performed. For example, chlorogenic acid shows potential antitumor activity by inhibiting DNA methyltransferase 1 (DNMT1) expression [30][31][32][33]. Thus far, several cancer clinical trial investigations have been performed for this compound.…”

Section: Discussionmentioning

confidence: 99%

“…Mechanistically, chlorogenic acid inhibited hepatocellular carcinoma growth by down-regulating DNA methyltransferase 1 (DNMT1) that assists with DNA methylation, the most prevalent epigenetic modification [32]. Interestingly, chlorogenic acid was also found to show a synergistic effect with the anticancer drug, doxorubicin, against human U2OS and MG-63 osteosarcoma cells [33].…”

Section: Potential Antitumor Activity Of Aronia Berriesmentioning

confidence: 99%

Potential Benefits of Black Chokeberry (Aronia melanocarpa) Fruits and Their Constituents in Improving Human Health

et al. 2022

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Aronia berry (black chokeberry) is a shrub native to North America, of which the fresh fruits are used in the food industry to produce different types of dietary products. The fruits of Aronia melanocarpa (Aronia berries) have been found to show multiple bioactivities potentially beneficial to human health, including antidiabetic, anti-infective, antineoplastic, antiobesity, and antioxidant activities, as well as heart-, liver-, and neuroprotective effects. Thus far, phenolic compounds, such as anthocyanins, cyanidins, phenolic acids, proanthocyanidins, triterpenoids, and their analogues have been identified as the major active components of Aronia berries. These natural products possess potent antioxidant activity, which contributes to the majority of the other bioactivities observed for Aronia berries. The chemical components and the potential pharmaceutical or health-promoting effects of Aronia berries have been summarized previously. The present review article focuses on the molecular targets of extracts of Aronia berries and the examples of promising lead compounds isolated from these berries, including cyanidin-3-O-galactoside, chlorogenic acid, quercetin, and ursolic acid. In addition, presented herein are clinical trial investigations for Aronia berries and their major components, including cancer clinical trials for chlorogenic acid and COVID-19 trial studies for quercetin. Additionally, the possible development of Aronia berries and their secondary metabolites as potential therapeutic agents is discussed. It is hoped that this contribution will help stimulate future investigations on Aronia berries for the continual improvement of human health.

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“…"The antitumor and toxic effects of DXR contribute to the production of free radicals and to the occurrence of oxidative stress" [85]. "In this context, phytochemical compounds have been described as an alternative to mitigate the harmful effects caused by DXR since metabolites from natural products have shown to be promising in overcoming the limitations of DXR in pre-clinical models such as chemosensitizers, chemoresistance inhibitors, and protectors chemotherapeutics in different types of cancer" [86,87].…”

Section: Mechanisms Of Antitumor Actionmentioning

confidence: 99%

Genus Vernonia (Asteraceae): A Promising Source of Antitumor Agents with Pharmacological Potentials

Gonzatto

Araújo²,

Albuquerque³

2022

AIR

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Antitumor research leads to the development of new molecules that act specifically in tumor cells by blocking or inhibiting their molecular targets. New therapeutic approaches for the screening of bioactive compounds present in medicinal plants have received increasing attention due to their chemopreventive and chemotherapeutic properties. In ethnomedicine, plants of the genus Vernonia (Asteraceae) are widely used and some have shown several and interesting biological activities, including anticancer. This present study aimed to document experimental evidence supporting the claimed ethnomedical uses of Vernonia species for the treatment of various types of cancer and also to confirm the anticancer potential of these plants. The compounds isolated from aqueous and alcoholic extracts, as well as fractions from different parts of Vernonia plants have acted as potential anticancer agents that inhibited the proliferation of various types of human cancer cell lines, including cervical cancer cells, melanoma cells, promyelocytic leucemia cells, breast adenocarcinoma cells, ovarian cancer, liver cancer cell, and human lung cancer cells. Studies have correlated the antitumor activity of Vernonia plants by inducing apoptosis and modulating mitochondrial signaling pathways controlled by NF-κB, Bcl-2 and p53, as well as inducing DNA damage and arresting the cell cycle at the S-phase checkpoint by oxidative stress. In conclusion, Vernonia species act as a promising source for drug development. However, further studies are needed to explore the exact mechanism of action, pharmacokinetics, chronic toxicological studies, safe dose consumption, and possible interactions with other herbs.

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Chlorogenic Acid Enhances Doxorubicin-Mediated Cytotoxic Effect in Osteosarcoma Cells

Cited by 26 publications

References 65 publications

Anti-Invasive and Anti-Migratory Effects of Ononin on Human Osteosarcoma Cells by Limiting the MMP2/9 and EGFR-Erk1/2 Pathway

Anti-Invasive and Anti-Migratory Effects of Ononin on Human Osteosarcoma Cells by Limiting the MMP2/9 and EGFR-Erk1/2 Pathway

Potential Benefits of Black Chokeberry (Aronia melanocarpa) Fruits and Their Constituents in Improving Human Health

Genus Vernonia (Asteraceae): A Promising Source of Antitumor Agents with Pharmacological Potentials

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