Chlorogenic acid in the oxidative stress injury triggered by Shuang‑Huang‑Lian injection

Abstract

Injections of Chinese herbs are a novel approach to prepare traditional Chinese medicines. However, as injections of Chinese herbs have been extensively used, adverse drug reactions (ADRs) have been on the increase. Additionally, the mechanism for injections of Chinese herbs remains unclear. This study explored the potential role played by chlorogenic acid (CGA) in initiating oxidative stress injury triggered by the utilization of injections of Chinese herbs and the underlying mechanism. A total of 90 male Wistar rats were raised for varying periods by using Shuang-Huang-Lian (SHL) injection or CGA in diverse dosages. Western blot analysis examined the expression of nicotinamide adenine dinucleotide phosphate oxidase subunits, spectrophotometry was used to examine the activity taken by catalase, ELISA was used to examine the concentrations of inflammatory factors in serum, and intravital microscopy was employed to examine the microcirculation. The results showed that the excessive peroxide production induced by CGA in high-dose or SHL in the venule walls may well be through nicotinamide adenine dinucleotide phosphate oxidase along with a decline in the activity of catalase, and led to imbalance of basal levels of pro-(TNF-α) and anti-(IL-10) inflammatory cytokines. On the basis of the aforementioned results, the mechanism hidden behind the adverse effects of CGA induced by irrational use of Chinese herbal injection can be identified from a deeper perspective.

Can coffee silverskin be a useful tool to fight metabolic syndrome?

“…The main bioactive compounds present in coffee silverskin (above detailed) can affect several pathways involved in the pathogenesis of MetS. In fact, they have been associated to beneficial effects on type II diabetes ( 40 , 41 ), obesity ( 42 ), insulin resistance ( 43 ), oxidative stress ( 40 , 44 ), regulation of glycemic and lipid disorders ( 45 ), and other MetS features. Those aspects and mechanisms will be presented and discussed below.…”

Comprehensive multicomponent characterization and quality assessment of Shuang‐Huang‐Lian powder injection using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight‐mass spectrometry and ultra‐high‐performance liquid chromatography‐quadrupole‐Orbitrap‐mass spectrometry

“…Chlorogenic acid has potential sensitizing activity. 21 Isochlorogenic acid B and isochlorogenic acid C are the isomers of neochlorogenic acid, so it is necessary to control their quantity. Based on these preliminary studies and our literature review, we decided to conduct quality analysis of these 10 compounds (neochlorogenic acid, chlorogenic acid, sweroside, forsythiaside A, luteoloside, isochlorogenic acid B, isochlorogenic acid C, baicalin, phillyrin, and baicalein).…”

“…Organic acids are the major constituents of honeysuckle. Chlorogenic acid has potential sensitizing activity 21 . Isochlorogenic acid B and isochlorogenic acid C are the isomers of neochlorogenic acid, so it is necessary to control their quantity.…”