Chlorogenic acid protects against intestinal inflammation and injury by inactivating the mtDNA–cGAS–STING signaling pathway in broilers under necrotic enteritis challenge

Inhibition of mtDNA-PRRs pathway-mediated sterile inflammation by astragalus polysaccharide protects against transport stress-induced cardiac injury in chicks

“…A previous study demonstrated that lycopene alleviates renal mitochondrial injury and inflammation induced by plasticizers through the cGAS-STING signaling pathway ( Li et al, 2023 ). Moreover, a study on necrotic enteritis in poultry has shown that dietary chlorogenic acid supplementation can prevent gut inflammation and damage by inhibiting the mtDNA-mediated cGAS-STING signaling pathway ( Lv et al, 2023 ), which supports our findings. In the present study, APS treatment restrained TS-induced activation of cGAS, STING, and IRF7 and their phosphorylation, further demonstrating the critical role of mtDNA-mediated cGAS-STING pathway in APS alleviating sterile inflammation induced by TS in chick heart.…”

“…As a key mtDNA sensing mediator, STING promotes IRF7 phosphorylation by activating TBK1 phosphorylation in Golgi. Then, IRF7 enters the nucleus and activates the innate immune response ( Barrera et al, 2021 ; Lv et al, 2023 ). A previous study demonstrated that lycopene alleviates renal mitochondrial injury and inflammation induced by plasticizers through the cGAS-STING signaling pathway ( Li et al, 2023 ).…”

“…The mtDNA was extracted from heart tissues according to the principles of the mitochondrial DNA extraction kit (PH1592, PHYGENE). Then, mtDNA levels were analyzed using quantitative real-time PCR ( qRT-PCR ), as previously reported ( Liang et al, 2023 ; Lv et al, 2023 ). The relative standard curve method was used to calculate mtDNA concentrations, and the primer sequences used in this research are shown in Table S1 (Supplementary material).…”

Chlorogenic Acid: A Systematic Review on the Biological Functions, Mechanistic Actions, and Therapeutic Potentials

“…CGA alleviates intestinal inflammation and injury in broilers induced by necrotic enteritis challenge through suppressing the mtDNA-cGAS-STING signaling pathway [337]. In a rat model of post-infectious irritable bowel syndrome (PI-IBS), rectal application of CGA ameliorated PI-IBS-related pathologies, probably by increasing glycine levels and modulating gut microbial-released extracellular vesicles [338].…”

“…Broilers induced by necrotic enteritis challenge [337]; a rat model of PI-IBS [338] Suppression of the mtDNA-cGAS-STING signaling pathway [337]; modulation of gut microbial-released extracellular vesicles [338] CGA Ovarian protection (Section 11.3) CDDP-induced ovarian damage in rats [339]; PCOS rats [340] HIF-1alpha signaling [340] CGA Menopausal symptom management (Section 11.4)…”