A b s t r a c tAnalysis of changes in chlorophyll fluorescence parameters in strawberry leaves was based on a field experiment performed in the years 2009-2010. Ten genotypes including 5 cultivars: 'Kent', 'Teresa', 'Senga Sengana', 'Chandler' and the breeding clone 1387 as well as their inbred progeny, were the object of the study. During the experiment the following indicators were evaluated: chlorophyll a and b content in fresh leaf mass as well as fluorescence parameters: minimum (F 0 ) and maximum fluorescence yield (F m ), photochemical efficiency of PS II (F v /F m ), actual quantum yield of PSII photochemistry (Y), minimum (F 0 ') and maximum efficiency of fluorescence (F m ') in the light, coefficient of photochemical (qP) and non-photochemical (qN) fluorescence quenching. In this work, we also examined the effect of repeated inbreeding on strawberry fruit yield and yield components. The analysis of changes of these parameters showed that inbreeding caused a reaction in all tested cultivars. In all inbred progeny, chlorophyll a and b content decreased compared to the cultivars. Generally, the photochemical efficiency of photosystem II (F v /F m ) and the parameter ΔF/ F m' were not affected by strong inbreeding. In analyzing the values of the coefficients qP and qN, it has been observed that changes in their values depend on the sensitivity of the examined genotypes to self-pollination. The functioning of PS II is the most sensitive indicator of the effect of various factors on plants and is useful, among others, in breeding to select plants with a required genotype.The yield -determining features such as: fruit yield per plant, weight of single fruit, number of fruit per plant and weight of leaves per plant in S 3 generation, were lower as compared with parental forms.