Chlorophyll fluorescence biosensor for the detection of herbicides

Merz, Daniela; Geyer, Michael; Moss, David A.; Ache, Hans J.

doi:10.1007/s0021663540299

Cited by 56 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The question whether or not the degrading bacteria could deal with such limiting amounts of the xenobiotic is of particular interest when placed in a bioremediation context. These concentrations pose a challenge for both the survival of the bacteria (Rapp and Timmis, 1999) and the analytical method used to detect the compound of interest (Merz et al, 1996). Because of the considerable costs, time and complexity of such analysis, we focused on an alternative approach based on the growth and chlorophyll fluorescence of L. minor (duckweed) to detect the presence/absence of nano-to micromolar concentrations of linuron.…”

Section: Discussionmentioning

confidence: 99%

A chlorophyll a fluorescence‐based Lemna minor bioassay to monitor microbial degradation of nanomolar to micromolar concentrations of linuron

Hulsen

Minne

Lootens

et al. 2002

Environmental Microbiology

View full text Add to dashboard Cite

A plant-microbial bioassay, based on the aquatic macrophyte Lemna minor L. (duckweed), was used to monitor biodegradation of nano- and micromolar concentrations of the phenylurea herbicide linuron. After 7 days of exposure to linuron, log-logistic-based dose-response analysis revealed significant growth inhibition on the total frond area of L. minor when linuron concentrations > or = 80 nM were added to the bioassay. A plant-protective effect was obtained for all concentrations > 80 nM by inoculation with either a bacterial consortium or Variovorax paradoxus WDL1, which is probably the main actor in this consortium. The outcome of the plant-microbe-toxicant interaction was also assessed using pulse amplitude-modulated chlorophyll a fluorescence and chlorophyll a fluorescence imaging. Linuron toxicity to L. minor became apparent as a significant decrease in the effective quantum yield (Delta F/Fm') within 90 min after exposure of the plants to linuron concentrations > or = 160 nM. Inoculation of the bioassay with the linuron-degrading bacteria neutralized the effect on the effective quantum yield at concentrations> or = 160 nM, indicating microbial degradation of these concentrations. The chlorophyll a fluorescence-based Lemna bioassay described here offers a sensitive, fast and cost-effective approach to study the potential of biodegrading microorganisms to break down minute concentrations of photosynthesis-inhibiting xenobiotics.

show abstract

Section: Discussionmentioning

confidence: 99%

A chlorophyll a fluorescence‐based Lemna minor bioassay to monitor microbial degradation of nanomolar to micromolar concentrations of linuron

Hulsen

Minne

Lootens

et al. 2002

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Merz et al, 1996;Beer and Bjork, 2000;Ralph, 2000;Juneau et al, 2002;Giardi et al, 2005). In 2001 the ToxY-PAM dual channel yield analyser was introduced as a new tool for the detection of phytotoxicants in the aquatic environment (Schreiber et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Biomaterial culture conditions impacting the performance of a PAM fluorometry based aquatic phytotoxicity assay

Nash

Quayle

2007

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…The herbicide biosensor is an example of an "activity" biosensor, in which a class of substances is detected on the basis of a common effect on the biological system used as the sensing element [15]. Public concern over the presence of pesticide residues in drinking water has led to the introduction of stringent limits on the maximum amounts permitted, but monitoring at these levels is a significant challenge for conventional methods of analysis, not at least because of the number and diversity of the substances involved.…”

Section: Biosensor Based Microanalysis Systemsmentioning

confidence: 99%

Chemical microanalytical systems: objectives and latest developments

Ache¹

1996

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

Chemical microanalysis systems are at a very early state of development. At the present time a modular approach with subsystems carrying out the various functions such as sampling, preparation and measurement seems to be favored by the developers. The marketpotential of the systems can be seen primarily in the area of inexpensive monitoring equipment for environmental and process control. Reduction of size leads also to smaller sample volumes, less waste and shorter analysis time: Miniaturization is in many cases an essential feature for biological and in-vivo medical investigations, while some properties inherently associated with miniaturization can also lead to substantial improvements in the technical performance of the microsystem as compared to its macro version. This will be demonstrated by discussing some microsystems which include spectroscopic detection systems:- Miniature separation instruments coupled with advanced miniaturized detection device based on photothermal spectroscopy- Integrated-optical NIR evanescent wave sensor systems- Optochemical and biosensor systems

show abstract

Chlorophyll fluorescence biosensor for the detection of herbicides

Cited by 56 publications

References 8 publications

A chlorophyll a fluorescence‐based Lemna minor bioassay to monitor microbial degradation of nanomolar to micromolar concentrations of linuron

A chlorophyll a fluorescence‐based Lemna minor bioassay to monitor microbial degradation of nanomolar to micromolar concentrations of linuron

Biomaterial culture conditions impacting the performance of a PAM fluorometry based aquatic phytotoxicity assay

Chemical microanalytical systems: objectives and latest developments

Contact Info

Product

Resources

About