Chlorophyll fluorescence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leaves

Ehlert, Britta; Hincha, Dirk K.

doi:10.1186/1746-4811-4-12

Cited by 138 publications

(107 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the exogenous application of maltose led to a reduced increase in conductivity in cold-sensitive plants such as OsMYB30-OE plants and some coldsensitive rice accessions, suggesting that maltose could reduce the damage to the cell membrane. Besides this, the chlorophyll fluorescence F v /F m ratio, which has been adopted as another physiological indicator reflecting the photosynthetic electron transport chain during cold stress treatment (Rosinski and Atchley, 1998;Baker and Rosenqvist, 2004;Ehlert and Hincha, 2008;Mishra et al, 2014;Thalhammer et al, 2014), was decreased in the rice plants with lower maltose accumulation (Fig. 4, E and F).…”

Section: Complexity Of Osmyb30 Regulation In Starch and Sugar Metabolismmentioning

confidence: 99%

“…According to previous studies, the increase in the maltose content could help in the protection of the photosynthetic electron transport chain Guy, 2004, 2005), in which the chlorophyll fluorescence F v /F m ratio has been used as an indicator to quantify cold tolerance in Arabidopsis, rice, and other plants (Baker and Rosenqvist, 2004;Ehlert and Hincha, 2008;Mishra et al, 2014;Thalhammer et al, 2014). We measured the F v /F m ratio after cold stress treatment, and the results showed that the F v /F m ratio was decreased significantly in the three OsMYB30-OE plants compared with ZH11 (Fig.…”

Section: Maltose May Contribute To Cell Membrane Protection During Comentioning

confidence: 99%

See 1 more Smart Citation

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

et al. 2017

View full text Add to dashboard Cite

Cold stress is one of the major limiting factors for rice (Oryza sativa) productivity. Several MYB transcriptional factors have been reported as important regulators in the cold stress response, but the molecular mechanisms are largely unknown. In this study, we characterized a cold-responsive R2R3-type MYB gene, OsMYB30, for its regulatory function in cold tolerance in rice. Functional analysis revealed that overexpression of OsMYB30 in rice resulted in increased cold sensitivity, while the osmyb30 knockout mutant showed increased cold tolerance. Microarray and quantitative real-time polymerase chain reaction analyses revealed that a few b-amylase (BMY) genes were down-regulated by OsMYB30. The BMY activity and maltose content, which were decreased and increased in the OsMYB30 overexpression and osmyb30 knockout mutant, respectively, were correlated with the expression patterns of the BMY genes. OsMYB30 was shown to bind to the promoters of the BMY genes. These results suggested that OsMYB30 exhibited a regulatory effect on the breakdown of starch through the regulation of the BMY genes. In addition, application of maltose had a protective effect for cell membranes under cold stress conditions. Furthermore, we identified an OsMYB30-interacting protein, OsJAZ9, that had a significant effect in suppressing the transcriptional activation of OsMYB30 and in the repression of BMY genes mediated by OsMYB30. These results together suggested that OsMYB30 might be a novel regulator of cold tolerance through the negative regulation of the BMY genes by interacting with OsJAZ9 to fine-tune the starch breakdown and the content of maltose, which might contribute to the cold tolerance as a compatible solute.

show abstract

Section: Complexity Of Osmyb30 Regulation In Starch and Sugar Metabolismmentioning

confidence: 99%

Section: Maltose May Contribute To Cell Membrane Protection During Comentioning

confidence: 99%

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The effect of altered COR15 protein levels on Arabidopsis freezing tolerance was tested using two independent approaches: an electrolyte leakage assay to assess freezing damage to the plasma membrane and the tonoplast (Rohde et al, 2004), and a chlorophyll fluorescence imaging assay to obtain information on the inactivation of photosynthesis and the intactness of chloroplast membranes (Ehlert and Hincha, 2008). As shown in Figure 2, freezing tolerance is expressed as the temperature at which 50% damage occurred or LT 50 .…”

Section: Cor15a and Cor15b Are Necessary For Arabidopsis To Attain Fumentioning

confidence: 99%

“…Chlorophyll fluorescence imaging analysis was performed on dark adapted leaves directly after thawing (i.e. omitting the incubation in distilled water), with freezing and thawing conditions identical to those described above, integrating measurements over the whole leaf area (Ehlert and Hincha, 2008). The LT 50 was calculated as the logEC 50 value derived from sigmoidal curves using GraphPad Prism3 software (Rohde et al, 2004).…”

Section: Determination Of Leaf Freezing Tolerancementioning

confidence: 99%

Disordered Cold Regulated15 Proteins Protect Chloroplast Membranes during Freezing through Binding and Folding, But Do Not Stabilize Chloroplast Enzymes in Vivo

et al. 2014

View full text Add to dashboard Cite

Freezing can severely damage plants, limiting geographical distribution of natural populations and leading to major agronomical losses. Plants native to cold climates acquire increased freezing tolerance during exposure to low nonfreezing temperatures in a process termed cold acclimation. This involves many adaptative responses, including global changes in metabolite content and gene expression, and the accumulation of cold-regulated (COR) proteins, whose functions are largely unknown. Here we report that the chloroplast proteins COR15A and COR15B are necessary for full cold acclimation in Arabidopsis (Arabidopsis thaliana). They protect cell membranes, as indicated by electrolyte leakage and chlorophyll fluorescence measurements. Recombinant COR15 proteins stabilize lactate dehydrogenase during freezing in vitro. However, a transgenic approach shows that they have no influence on the stability of selected plastidic enzymes in vivo, although cold acclimation results in increased enzyme stability. This indicates that enzymes are stabilized by other mechanisms. Recombinant COR15 proteins are disordered in water, but fold into amphipathic a-helices at high osmolyte concentrations in the presence of membranes, a condition mimicking molecular crowding induced by dehydration during freezing. X-ray scattering experiments indicate protein-membrane interactions specifically under such crowding conditions. The COR15-membrane interactions lead to liposome stabilization during freezing. Collectively, our data demonstrate the requirement for COR15 accumulation for full cold acclimation of Arabidopsis. The function of these intrinsically disordered proteins is the stabilization of chloroplast membranes during freezing through a folding and binding mechanism, but not the stabilization of chloroplastic enzymes. This indicates a high functional specificity of these disordered plant proteins.

show abstract

“…The moderate correlation obtained between clonal electrolytic leakage and photosystem efficiency following artificial freezing was not surprising, as the two methods take into account different parameters and while electrolyte leakage can be considered an estimate of the cellular membrane damage, F v /F m shows the efficiency of photosystem II, which can be impaired even without physical membrane damage (Saibo et al 2009;Yamamoto et al 2008). The two techniques were used to measure cold damage at different freezing temperatures in Arabidopsis (Ehlert and Hincha 2008) and while a general correspondence was found, differences between the two methods were highlighted. According to the results presented in this paper, most of rank changing was observed for those accessions showing average cold tolerance.…”

Section: Discussionmentioning

confidence: 99%

Cold tolerance in cypress (Cupressus sempervirens L.): a physiological and molecular study

Baldi

Pedron

Hietala³

et al. 2010

Tree Genetics & Genomes

View full text Add to dashboard Cite

Twenty cypress accessions were tested for freezing tolerance. After freezing to −15°C, differences among cypress accessions were tested by measuring electrolyte leakage and chlorophyll fluorescence. Based on these data, cypress accessions showing contrasting freezing tolerance were subjected to transcript profiling of candidate genes upon the development of cold hardening, with the ultimate goal of providing a scientific basis for selecting/breeding cypress genotypes with higher tolerance to low temperature. Nine different cypress genes were selected: a heat shock protein, a putative chaperonin, a chlorophyll-binding protein, a serine/threonine protein kinase, a putative exonuclease, a dehydrin, and three senescenceassociated proteins. Transcript levels of these genes were profiled during cold hardening under controlled conditions using real-time reverse-transcription-polymerase chain reaction. While the genes showed regulation patterns common to both cypress accessions, in the case of chaperonin, exonuclease, and some senescence-associated proteins, clonal differences in gene regulation were found. The potential relationship of these differences with cold tolerance is discussed.

show abstract

Chlorophyll fluorescence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leaves

Cited by 138 publications

References 34 publications

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

Disordered Cold Regulated15 Proteins Protect Chloroplast Membranes during Freezing through Binding and Folding, But Do Not Stabilize Chloroplast Enzymes in Vivo

Cold tolerance in cypress (Cupressus sempervirens L.): a physiological and molecular study

Contact Info

Product

Resources

About