Chlorophyll <i>A</i> Fluorescence and Chlorophyll Content in <i>Parmelia Quercina</i> Thalli from a Polluted Region of Northern Castellon (Spain)

Calatayud, Ángeles; Sanz, M. J.; Calvo, Esperanza; Barreno, Eva; Valle-Tascón, Secundino del

doi:10.1006/lich.1996.0005

Cited by 39 publications

(22 citation statements)

References 44 publications

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“…Another study [26] reported on the effects of ozone fumigation and revealed a significant reduction of the variable fluorescence/maximal fluorescence (F v /F m ) ratio in five of the seven investigated macrolichens, thus indicating severe stress on photosystem II (PSII) due to ozone. A study of chlorophyll a fluorescence carried out in Spain detected a similar F v /F m for thalli in polluted regions and for control samples [27]. Another study on this subject carried out in Poland found significantly lowered fluorescence parameters in three lichen species from the more polluted site [28].…”

Section: Introductionmentioning

confidence: 81%

Changes in the Potential Quantum Yield of Photosystem Ii and the Integrity of Cell Membranes Relative to the Elemental Content of the Epilithic Desert Lichen Ramalina Maciformis

Garty¹,

Tamir²,

Cohen³

et al. 2002

Environ Toxicol Chem

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Abstract-The present study used the epilithic fruticose lichen Ramalina maciformis to investigate the occurrence of mineral elements, including heavy metals, at a distance of up to 50 km from the industrial region in Ramat Hovav in the Negev Desert, Israel. The major objective of this study was an analytical comparison of elemental content and physiological parameters of lichen vitality, apart from a test of the applicability of this specific lichen in investigations of air pollution. The Ca, Cr, Cu, K, Mg, Na, Pb, S, Sr, and Zn content of thalli from the unpolluted Tellalim site collected in August 1997, transferred to 24 biomonitoring sites, and retrieved in April 1998 was analyzed in comparison with the following parameters: The potential quantum yield of photosystem II (PSII), and the integrity of cell membranes. Transplanted thalli in several sites at Ramat Hovav accumulated large amounts of most of the elements. The K content of the transplants located in the polluted sites indicated a leakage of this element, because this content was lower than that of thalli in unpolluted sites. Calcium, Cu, Mn, and Na showed an inverse correlation with the K content of the lichen. Calcium, Cu, and Sr showed an inverse correlation with the F v /F m ratio expressing the potential quantum yield of PSII. Calcium, Cr, Cu, Mg, Na, S, and Sr showed a positive correlation with the electrical conductivity corresponding with cell-membrane disintegration. The present study demonstrated a meaningful connection between enlarged concentrations of certain elements and physiological phenomena. The capability of the lichen to detect air pollution was found to be satisfactory. The dispersion of airborne heavy metals was found, however, to be local and limited to a few hundred meters from the source of pollution.

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Section: Introductionmentioning

confidence: 81%

Changes in the Potential Quantum Yield of Photosystem Ii and the Integrity of Cell Membranes Relative to the Elemental Content of the Epilithic Desert Lichen Ramalina Maciformis

Garty¹,

Tamir²,

Cohen³

et al. 2002

Environ Toxicol Chem

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“…An additional study of the impact of a chemical pollutant on Chl fluorescence in dark-adapted lichens revealed an impairment of PSII photochemical reactions, measured as a decrease in F v /F m as a result of Pb uptake, particularly in cyanobiont lichens (Branquinho et al 1997b). On the other hand, a study of Chl a fluorescence in lichen thalli, from a polluted region in Spain, revealed a F v /F m ratio that was about the same as for control samples (Calatayud et al 1996).…”

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confidence: 87%

Comparison of five physiological parameters to assess the vitality of the lichen Ramalina lacera exposed to air pollution

Garty

Weissman

Tamir

et al. 2000

Physiologia Plantarum

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To determine the environmental impact of industrial activity, we examined and compared the following parameters of physiological integrity in the epiphytic fruticose lichen Ramalina lacera. (1) Potential quantum yield of PSII expressed as the chlorophyll fluorescence ratio Fv/Fm. (2) Spectral reflectance expressed as values of normalized difference vegetation index (NDVI). (3) Production of ethylene. (4) Electrolyte leakage brought about by degrading cell membranes in terms of electric conductivity. (5) The ratio A435nm/A415nm indicating the disintegration of chlorophyll. The amounts of Ba, Cu, K, Ni, S, V and Zn contained in the lichen thallus were measured to quantify the degree of pollution. Some of the lichen‐carrying twigs collected at a relatively unpolluted control site were resuspended on the original trees as controls. Other lichens were transplanted to 19 biomonitoring sites. Transplanted lichens in polluted sites contained higher amounts of Ba, Ni, S, V and Zn than lichens in the control site and in most of the rural sites upon an exposure period of 8 months. Statistical analysis revealed negative correlations between Fv/Fm ratios and Ba contents of transplants. High Ba, Cu, Ni and Zn contents correlated negatively with NDVI values. NDVI values correlated with A435nm/A415nm, Fv/Fm and K. The ratio A435nm/A415nm correlated positively with K and negatively with Ba, Cu and Zn. The production of ethylene correlated positively with Cu and Ba and negatively with A435nm/A415nm, Fv/Fm and NDVI. Electric conductivity values correlated positively with Ba, Cu, Na, S, V and Zn and negatively with NDVI. Both elemental content and physiological alterations in transplants of R. lacera point to a high degree of contamination in the Haifa Bay region, which is polluted by fuel‐oil combustion apart from other industrial activities. The present work suggests that in our specific study area, the most sensitive parameter to assess the vitality of the lichen thallus is electric conductivity whereas ethylene production is less sensitive.

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“…There is a substantial body of evidence that NPQ may reflect the capacity of plants and lichens to carry out non‐radiative dissipation of excess energy ( Demmig‐Adams et al 1990). Previous work from our laboratory with the lichen P. quercina from polluted and control sites has shown that NPQ is affected by the presence of phytotoxic pollutants ( Calatayud et al 1996). Thus, environmental stresses might also affect heat emission and reduce the ability for safely dissipating absorbed energy.…”

Section: Discussionmentioning

confidence: 99%

“…The sampling grounds fall within the area covered by a government air monitoring network, so that a continuous record on atmospheric pollutants is available since 1991. High O 3 concentrations have been recorded for long periods in the area as a result of complex photochemical processes ( Calatayud et al 1996). The experimental sites were selected after taking into account different variables, such as visible damage to surrounding vegetation and lichens, direction of prevailing winds and proximity to monitoring stations.…”

Section: Methodsmentioning

confidence: 99%

“…Exposure of plants to pollutant stress has been shown to decrease antioxidant levels ( Luwe et al 1993; Nouchi 1993; Wellburn et al 1996). Lichens have long been recognized to be sensitive to air pollutants, and a considerable amount of literature exists concerning air pollution impact on these organisms ( Gries et al 1995; Balaguer et al 1996; Calatayud et al 1996; Garty et al 1997; Gries et al 1997). However, scant attention has been paid to how atmospheric pollutants interfere with the ability of lichens to maintain antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

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Effects of ascorbate feeding on chlorophyll fluorescence and xanthophyll cycle components in the lichen Parmelia quercina (Willd.) Vainio exposed to atmospheric pollutants

Calatayud¹,

Deltoro²,

Abadı́a

et al. 1999

Physiologia Plantarum

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The effects of environmental stresses on photosynthetic responses, ascorbate levels and pigment composition were investigated in samples of Parmelia quercina (Willd.) Vainio from control and polluted regions of the northern Castellón area (Valencia, Spain). In response to sustained pollutant stress in the field, lichen thalli had closed PSII traps and exhibited lower rates of electron transport and non‐radiative energy dissipation. The xanthophyll concentration was not affected by exposure to atmospheric pollutants. The ascorbate concentration was lower in samples exposed to ambient air pollutants compared to control thalli. Ascorbate feeding of thalli from polluted sites stimulated electron flow, photochemical quenching and non‐radiative energy dissipation. Additionally, ascorbate feeding enhances the de‐epoxidation state of the xanthophyll pool in polluted thalli. The partial recovery for non‐radiative energy dissipation was presumably due to the interaction between the increased thylakoid pH gradient and de‐epoxidized xanthophylls. Furthermore, ascorbate feeding decreased photon excess in thalli from polluted sites owing to the stimulation of linear electron flow and non‐radiative energy dissipation. The present study suggests that atmospheric pollutants, besides their intrinsic toxicity, put on an additional burden by hampering the operation of photoprotective mechanisms.

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Chlorophyll A Fluorescence and Chlorophyll Content in Parmelia Quercina Thalli from a Polluted Region of Northern Castellon (Spain)

Cited by 39 publications

References 44 publications

Changes in the Potential Quantum Yield of Photosystem Ii and the Integrity of Cell Membranes Relative to the Elemental Content of the Epilithic Desert Lichen Ramalina Maciformis

Changes in the Potential Quantum Yield of Photosystem Ii and the Integrity of Cell Membranes Relative to the Elemental Content of the Epilithic Desert Lichen Ramalina Maciformis

Comparison of five physiological parameters to assess the vitality of the lichen Ramalina lacera exposed to air pollution

Effects of ascorbate feeding on chlorophyll fluorescence and xanthophyll cycle components in the lichen Parmelia quercina (Willd.) Vainio exposed to atmospheric pollutants

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