Chlorophyll a might structure a community of potentially pathogenic culturable Vibrionaceae. Insights from a one‐year study of water and mussels surveyed on the French Atlantic coast

Abstract

The present study focused on the isolation of culturable bacteria from mussels and sea water to identify Vibrionaceae potentially pathogenic for humans. Three sites located on the French Atlantic coast were monitored monthly (twice each month during summer) for 1 year. Environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by API 20E(®) systems (BioMérieux) and PCR. A total of seven species were detected (Grimontia hollisae, Photobacterium damselae, Vibrio alginolyticus, V. cholerae, V. fluvialis, V. vulnificus and V. parahaemolyticus) and species diversity was higher at the end of summer. Surprisingly, V. cholerae non-O1/non-O139 was detected in spring. No site effect was detected. Using Sørensen similarity indices and statistical analyses, we showed that chlorophyll a had a significant influence on the bacterial community detected in mussels and assemblages were more similar to one another when chlorophyll a values were above 20 µg l(-1) . No significant effect of any parameter was found on the community detected in water samples. Such surveys are essential for the understanding of sanitary crises and detection of emerging pathogens.

Occurrence of Vibrio parahaemolyticus and Vibrio alginolyticus in the German Bight over a seasonal cycle

“…About 80% of the isolates were classified as V. alginolyticus, which is in agreement with other studies from European waters (Cavallo and Stabili 2002;Lhafi and Kühne 2007). The second most abundant culturable Vibrio species was the potential human pathogen, V. parahaemolyticus, an observation likewise supported by previous studies (Lhafi and Kühne 2007;Deter et al 2010a). The V. alginolyticus isolates from Helgoland Roads could be divided into two genetically distinguishable groups, which was supported by the results of the ERIC-PCR fingerprinting and the ANOSIM calculations (Fig.…”

“…All three Vibrio groups (V. parahaemolyticus, V. alginolyticus I and II) coexisted solely in the summer months, indicating a higher diversity concerning Vibrio spp. A study by Deter et al (2010a) in northern France corroborates this observation. The authors detected the highest biodiversity within the Vibrio community in summer.…”

Environmental parameters influence on the dynamics of total and pathogenic Vibrio parahaemolyticus densities in Crassostrea virginica harvested from Mexico’s Gulf coast

“…It is commonly found free swimming, attached to underwater surfaces, and commensally associated with various species of shellfish (Deter et al, 2010;Turner et al, 2014). V. parahaemolyticus has recently been recognized as one of the most important emerging foodborne pathogen and as the leading causal agent of human acute gastroenteritis, primarily following the consumption of raw, undercooked or mishandled seafood and marine products, particularly oysters Wong et al, 2000;Duan and Su, 2005;Martinez-Urtaza et al, 2005;Yano et al, 2006;Su and Liu, 2007;Di Pinto et al, 2008;Pal and Das, 2010;Velázquez-Roman et al, 2012).…”

Influence of Environmental Factors onVibriospp. in Coastal Ecosystems

“…parahaemolyticus in various niches has been correlated with temperature. Total V. parahaemolyticus in water has been positively correlated with temperature in the northern Gulf of Mexico (37,38,39), Maryland (40), North Carolina (31), Chesapeake Bay (41), Washington (37), Oregon (42), Japan (43), France (44,45), India (46), and Germany (47) ( Table 3). Total V. parahaemolyticus in other sample types has also been examined; temperature has been positively correlated with total V. parahaemolyticus in oysters (37,38,39,42,48,49) and in sediment (31,37,41,42,47).…”