Chlorophylls and carotenoids of colourless callus, green callus and leaves of kalanchoë crenata

Stobart, A. K.; Mclaren, I.; Thomas, David

doi:10.1016/s0031-9422(00)82940-4

Cited by 20 publications

(3 citation statements)

References 10 publications

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“…In addition, many plant tissue cultures will synthesize chloroplast pigments when grown in light (Powell, 1925;Stobart et al, 1967). Roots, whether excised or attached to the plant, can be induced to form chloroplasts by exposure to light (Powell, 1925;Bjorn, 1963;Heltne and Bonnett, 1970; Bajaj and McAllan, 1969).…”

mentioning

confidence: 99%

Light-dependent carotenoid synthesis in the tomato fruit

Raymundo¹,

Chichester²,

Simpson³

1976

J. Agric. Food Chem.

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mentioning

confidence: 99%

Light-dependent carotenoid synthesis in the tomato fruit

Raymundo¹,

Chichester²,

Simpson³

1976

J. Agric. Food Chem.

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“…Under the cultural conditions described in this report, other chloroplast pigments were formed. All phenotypes contained the principal carotenoids (a-and f3-carotene, lutein-zeaxanthin, violaxanthin and neoxanthin) that are also found in the NG and LG parent phenotypes (Keck et aI., 1970) and in other plant cultural systems (Williams and Goodwin, 1965;Stobart, McLaren, and Thomas, 1967;Davey, Fowler, and Street, 1971). Existence of measurable quantities of a-and f3-carotene in the Y callus phenotype which are not found in leaf material of the parent plant (Keck et aI., 1970) indicated that an exogenous carbon supply supported a low level of carotenoid synthesis in the Y callus phenotype when grown at 3,000 lux.…”

Section: Results-different Exogenous Carbohydrates Produced Varied Efmentioning

confidence: 90%

Chlorophyll and Carotenoid Accumulation in Three Chlorophyllous Callus Phenotypes of Glycine Max

Hemphill

Venketeswaran

1978

American J of Botany

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Effects of exogenous carbohydrates and various medium supplements on chlorophyll and carotenoid accumulation in three chlorophyllous callus phenotypes of Glycine max (L.) Merrill were studied. Glucose (filtered), at 3%, supported the highest level of chlorophyll and carotenoids in the NG and Y phenotypes, while only moderate levels of chlorophyll accumulated in the LG phenotype. Sucrose (filtered and autoclaved), at 3%, supported phenotypical levels of chlorophyll and carotenoids for all phenotypes. Ascorbic acid, at 75 mg/l, stimulated chlorophyll‐carotenoid accumulation for all phenotypes, while δ‐aminolevulinic acid was slightly toxic for pigment biosynthesis in the NG and LG phenotypes. In contrast, δ‐aminolevulinic acid supported chlorophyll‐carotenoid accumulation in the Y phenotype. A negative correlation (rXY) was evident between chlorophyll formation and callus growth for all phenotypes. Light intensity of 3,000 lux suppressed chlorophyll accumulation in the Y callus phenotype while an increase in pigment formation occurred in the NG and LG callus phenotypes. In comparison, light intensity of 700 lux supported chlorophyll accumulation in the Y callus phenotype. Carotenoid accumulation appeared to be coupled with chlorophyll formation in all callus phenotypes except for the Y callus phenotype when grown under the higher light intensity. All phenotypes accumulated chlorophylls a and b, α‐ and β‐carotene, lutein plus zeaxanthin, violaxanthin and neoxanthin as indicated by their respective absorption maxima. Under cultural conditions, the genetic mutation (yll) in these soybean callus phenotypes appeared to regulate carotenoid formation which in turn influenced chlorophyll stability.

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“…It is a common finding that when etiolated plants are exposed to light a lag phase in the biosynthesis of chlorophyll is found (Virgin, 1955(Virgin, , 1960Wolf & Price, 1957) and then a rapid synthesis of chlorophyll occurs (Sisler & Klein, 1963). However, some plant tissue cultures form chlorophyll in the light at a low rate (Sunderland, 1966;Stobart, McLaren & Thomas, 1967). The early effects of illumination on chlorophyll development in such chlorophyllous tissues in culture have not been reported.…”

Section: Exptmentioning

confidence: 99%

Studies on the porphobilinogen deaminase–uroporphyrinogen cosynthetase system of cultured soya-bean cells

Llambías

Batlle

1971

Biochemical Journal

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1. Porphobilinogenase was isolated and purified from soya-bean callus tissue; its components, porphobilinogen deaminase and uroporphyrinogen isomerase, were separated and purified. 2. The purified porphobilinogenase was resolved into two bands on starch-gel electrophoresis. The molecular weights of porphobilinogenase, deaminase and isomerase fractions were determined by the gel-filtration method. Porphobilinogenase activity was affected by the presence of air; uroporphyrinogens were only formed under anaerobic conditions, although substrate consumption was the same in the absence of oxygen as in its presence. 3. pH-dependence of both porphobilinogenase and deaminase was the same and a sharp optimum at pH 7.2 was obtained. Isomerase was heat-labile, but the presence of ammonium ions or porphobilinogen afforded some protection against inactivation. The action of several compounds added to the system was studied. Cysteine, thioglycollate, ammonium ions and hydroxylamine inhibited porphobilinogenase; certain concentrations of sodium and magnesium salts enhanced activity; some dicarboxylic acids and 2-methoxy-5-nitrotropone inhibited the deaminase. 4. delta-Aminolaevulate and ethionine in the culture media stimulated porphyrin synthesis and increased porphobilinogenase activity, whereas iron deficiency resulted in porphyrin accumulation. 5. The development of chlorophyll and porphobilinogenase on illumination of dark-grown callus was followed. 6. A hypothetical scheme is suggested for the enzymic synthesis of uroporphyrinogens from porphobilinogen.

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Chlorophylls and carotenoids of colourless callus, green callus and leaves of kalanchoë crenata

Cited by 20 publications

References 10 publications

Light-dependent carotenoid synthesis in the tomato fruit

Light-dependent carotenoid synthesis in the tomato fruit

Chlorophyll and Carotenoid Accumulation in Three Chlorophyllous Callus Phenotypes of Glycine Max

Studies on the porphobilinogen deaminase–uroporphyrinogen cosynthetase system of cultured soya-bean cells

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