Chloroplast and nuclear DNA analyses provide insight into the phylogeography and conservation genetics of Camellia nitidissima (Theaceae) in southern Guangxi, China

Lu, Xuelin; Chen, Hailing; Bin, Xiaoyun; Ye, Quanqing; Tang, Shaoqing

doi:10.1007/s11295-019-1390-1

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…The π values of the tested populations revealed by the five chloroplast sequences varied widely, ranging from 0.00013 to 0.00502, with an average value of 0.00140 (Table 1). This is similar to the levels of genetic diversity observed in other Camellia species, such as Camellia huana (π of 0.00042; Li et al, 2020), Camellia flavida (π of 0.00157; Wei et al, 2017), and Camellia nitidissima (π of 0.00082; Lu et al, 2020). However, the π values of most species are between 0.0020 and 0.0058 (Chen et al, 2012;Xing et al, 2017), relative to which the genetic diversity level of the population tested herein is low.…”

Section: Molecular Identification Of Tea-oil Camellia Germplasm Resou...supporting

confidence: 85%

Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China

Sun

Yan

et al. 2020

Front. Plant Sci.

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Tea-oil Camellia is one of the four woody oil crops in the world and has high ecological, economic and medicinal values. However, there are great differences in the classification and merging of tea-oil Camellia Sect. Oleifera species, which brings difficulties to the innovative utilization and production of tea-oil Camellia resources. Here, ISSR, SRAP and chloroplast sequence markers were analyzed in 18 populations of tea-oil Camellia Sect. Oleifera species to explore their phylogenetic relationships and genetic diversity. The results showed that their genetic diversity were low, with mean H and π values of 0.16 and 0.00140, respectively. There was high among-population genetic differentiation, with ISSR and SRAP markers showing an Fst of 0.38 and a high Nm of 1.77 and cpDNA markers showing an Fst of 0.65 and a low Nm of 0.27. The C. gauchowensis, C. vietnamensis and Hainan Island populations formed a single group, showing the closest relationships, and supported being the same species for them with the unifying name C. drupifera and classifying the resources on Hainan Island as C. drupifera. The tea-oil Camellia resources of Hainan Island should be classified as a special ecological type or variety of C. drupifera. However, cpDNA marker-based STRUCTURE analysis showed that the genetic components of the C. osmantha population formed an independent, homozygous cluster; hence, C. osmantha should be a new species in Sect. Oleifera. The C. oleifera var. monosperma and C. oleifera populations clustered into two distinct clades, and the C. oleifera var. monosperma populations formed an independent cluster, accounting for more than 99.00% of its genetic composition; however, the C. oleifera populations contained multiple different cluster components, indicating that C. oleifera var. monosperma significantly differs from C. oleifera and should be considered the independent species C. meiocarpa. Haplotype analysis revealed no rapid expansion in the tested populations, and the haplotypes of C. oleifera, C. meiocarpa and C. osmantha evolved from those of C. drupifera. Our results support the phylogenetic classification of Camellia subgenera, which is highly significant for breeding and production in tea-oil Camellia.

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Section: Molecular Identification Of Tea-oil Camellia Germplasm Resou...supporting

confidence: 85%

Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China

Sun

Yan

et al. 2020

Front. Plant Sci.

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“…(2019) Camellia nitidissima 3.881 0.546 7 0.837 0.00082 Li et al. (2019) ; Lu et al. (2020) Camellia nitidissima var.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to C. huana , many other yellow-flowered Camellia species, such as Camellia chrysanthoides H. T. Chang ( Chen et al., 2019 ), Camellia micrantha S. Y. Liang et Y. C. Zhong ( Chen et al., 2019 ), Camellia parvipetala J. Y. Liang et Su ( Chen et al., 2019 ), Camellia nitidissima Chi ( Li et al., 2019 , Lu et al., 2020 ) and C. nitidissima Chi var. microcarpa Chang et Ye ( Lu et al., 2019 ), exhibit a small distribution range and low level of genetic diversity ( Table 5 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Shang-li

Pei

et al. 2020

Plant Diversity

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Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity ( n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, N A = 3.931, H E = 0.466 for SSRs), but high genetic differentiation between populations ( F ST = 0.2159 for SSRs, F ST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana . STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.

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“…Cp-derived markers were successfully employed to study the evolutionary relationships of plants in sects. Thea , Camellia , Theopsis , Stereocarpus , Chrysantha , and Corallina , etc., providing valuable genetic information for accurate identification of species and reconstruction of the phylogeny of the genus Camellia [ 6 , 11 , 12 , 13 , 14 , 23 , 24 , 25 , 26 , 27 ]. However, the lack of suitable polymorphic genetic markers has obstructed the phylogenic analysis of sect.…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analysis Uncovers the Chloroplast Genome Variation and Phylogenetic Relationships of Camellia Species

Lin

Yin²,

Wang³

et al. 2022

Biomolecules

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Camellia is the largest genus in the family Theaceae. Due to phenotypic diversity, frequent hybridization, and polyploidization, an understanding of the phylogenetic relationships between Camellia species remains challenging. Comparative chloroplast (cp) genomics provides an informative resource for phylogenetic analyses of Camellia. In this study, 12 chloroplast genome sequences from nine Camellia species were determined using Illumina sequencing technology via de novo assembly. The cp genome sizes ranged from 156,545 to 157,021 bp and were organized into quadripartite regions with the typical angiosperm cp genomes. Each genome harbored 87 protein-coding, 37 transfer RNA, and 8 ribosomal RNA genes in the same order and orientation. Differences in long and short sequence repeats, SNPs, and InDels were detected across the 12 cp genomes. Combining with the complete cp sequences of seven other species in the genus Camellia, a total of nine intergenic sequence divergent hotspots and 14 protein-coding genes with high sequence polymorphism were identified. These hotspots, especially the InDel (~400 bp) located in atpH-atpI region, had sufficient potential to be used as barcode markers for further phylogenetic analysis and species identification. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp genomes in this study. These cp genomes could facilitate the development of new molecular markers, accurate species identification, and investigations of the phylogenomic relationships of the genus Camellia.

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Chloroplast and nuclear DNA analyses provide insight into the phylogeography and conservation genetics of Camellia nitidissima (Theaceae) in southern Guangxi, China

Cited by 8 publications

References 40 publications

Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China

Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Comparative Genomic Analysis Uncovers the Chloroplast Genome Variation and Phylogenetic Relationships of Camellia Species

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