Chloroplast Development in Nicotiana Tabacum ‘Maryland Mammoth’

Abstract

The development of chloroplasts in light‐grown and in previously etiolated tissues of tobacco has been studied. A single membrane‐bound body is found in the developing plastids of both light‐ and dark‐grown tissue. The contents of the body appear homogeneous, becoming progressively granular as the chloroplast develops. In the mature chloroplast the body contains a fibrillar network resembling strands shown to be DNA by other workers. The prolamellar body persists even in moderately well developed chloroplasts in light‐grown plants. Frequently the prolamellar body is connected to the membrane‐bound body as well as to the grana. Relatively mature chloroplasts are seen to divide in this tissue. The membrane‐bound body may have a role in the formation of lamellae, but the nature of its contents is yet to be determined.

Etioplast and etio-chloroplast formation under natural conditions: the dark side of chlorophyll biosynthesis in angiosperms

“…However, the actual value of the threshold light intensity under which PLBs develop depends on the experimental conditions, including the light source used, the plant age and taxon. PLBs occur mostly in young and nonmature cells having an active Mg-tetrapyrrole biosynthetic activity also in this case (Casadoro and Rascio 1987;Halloin et al 1970;Laetsch and Price 1969;Platt-Aloia and Thomson 1977;Rascio et al 1980;Signol 1961;Solymosi et al 2007;Stetler and Laetsch 1969). In these, greening samples grown under low light intensity etiochloroplasts with grana and in general with more than one PLB have been observed (Fig.…”

Plastid Ultrastructure in Leaves ofMacrozamia Moorei(Zamiaceae)

“…In tobacco, Coleus and sesame membrane-bound inclusions, mainly proteic in composition (.AMES and P:rvoRUN 1974), were found in immature plastids and gradually disappeared during chloroplast development (STETLER and LAETSCH 1969;MARTY 1973MARTY -1974HuRKMAN and KENNEDY 1977;PLATT-ALOIA and THOMSON 1977).…”

A membrane-bound plastid inclusion in the epidermis of leaves of Taraxacum officinale

“…There have been a number of recent reports on the occurrence of membrane-bound inclusion bodies in plastids (Ames 1972;Stetler and Laetsch 1969;Boasson et al 1972). These inclusion bodies have been observed in a wide range of different tissues, but generally consist of electron-dense granular material that is enclosed by a single membrane.…”