Chloroplast Genes in Higher Plants

Scott, N. Steele; Jn, Timmis; Ayliffe, MA

doi:10.1071/pp9910533

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As with the P‐Ract1 promoter segment, the rps 11/ rpo A segment is not expected to be a significant contributor to regulation of the transgene or surrounding genomic DNA. First, it lacks the endogenous plastid promoter of the rpl 23 gene cluster and second, if cryptic regulatory sequences did exist, they would function poorly in a nuclear context (Cornelissen and Vandewiele, 1989; Scott et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event

et al. 2005

View full text Add to dashboard Cite

5‐Enol‐pyruvylshikimate‐3‐phosphate synthase from Agrobacterium sp. CP4 (CP4 EPSPS) confers tolerance to the nonselective herbicide glyphosate (marketed under the trade name Roundup1) when sufficiently expressed in transgenic plants. Dual CP4 EPSPS transgene cassettes were transformed into corn (Zea mays L.) under the transcriptional regulatory control of the rice (Oryza sativa L.) actin 1 (P‐Ract1) and the enhanced Cauliflower mosaic virus 35S (P‐e35S) promoters, respectively, to impart fully constitutive expression in corn. Resulting events were tested for lack of chlorosis and malformation injury after two sequential applications of 1.68 kg acid equivalents (a.e.) ha−1 glyphosate. Agronomic parameters, male fertility, appropriate Mendelian segregation of the trait, plus characteristics of the transgenic integration site were also evaluated. From this selection process, the NK603 event was chosen for commercialization as the event that embodied the most optimal profile of tolerance, agronomics, and molecular characteristics. The NK603 event exhibited high glyphosate tolerance from one transgenic locus bearing a single copy of the dual cassettes integrated into the corn genome with a minimum of target sequence disruption. Trait expression in the NK603 event has remained stable over more than eight generations as shown through tolerance testing, western blots of CP4 EPSPS accumulation, and Southern blot analysis of the transgene.

show abstract

Section: Resultsmentioning

confidence: 99%

Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event

et al. 2005

View full text Add to dashboard Cite

show abstract

“…However, this structural detail may be the remnant of a primeval plastid origin of phytochrome or an accidental arrangement. (For a discussion of similar findings see Scott et al 1991). Peculiarities are also detected in the first intron.…”

Section: Fern Phytochromesmentioning

confidence: 68%

Phytochrome evolution: Phytochrome genes in ferns and mosses

Schneider‐Poetsch

Marx

Kolukisaoglu

et al. 1994

Physiologia Plantarum

View full text Add to dashboard Cite

We have isolated phytochrome genes from the moss Physcomitrella, the fern Psilotum and PCR‐generated phytochrome sequences from a few other ferns. The phytochrome gene of the moss Physcomitrella turned out not to contain the aberrant C‐terminal third of the phytochrome from the moss Ceratodon, but the transmitter module‐like sequences found in other phytochromes. A series of different phytochrome genes was detected in Psilotum. Differences between the amino acid sequences derived from them ranged from about 5 to more than 22%. Some of these genes are likely pseudogenes. Analysis by phylogenetic tree constructions revealed that higher and lower plant phytochromes evolved with different velocities. Lower plant phytochromes form a separate family characterized by a high degree of similarity. The amino acid differences between phytochrome types detected in a single species of higher plants are about two‐fold higher than the differences between phytochromes of species of lower plants belonging to different divisions (Physcomitrella and Selaginella). Future studies on phytochrome sequences may eventually also throw light on the significance of Psilotum in the evolution of vascular plants.

show abstract

“…(For a discussion of similar findings seeScott et al 1991). Peculiarities are also detected in the first intron.…”

mentioning

confidence: 60%

Phytochrome evolution: Phytochrome genes in ferns and mosses

Schneider-Poetsch¹,

Marx²,

Kolukisaoglu³

et al. 1994

Physiol Plant

View full text Add to dashboard Cite

Phytochrome evolution: Phytochrome genes in ferns and mosses. -Physiol. Plant. 91: 241-250.We have isolated phytochrom'e genes from the moss Physcomitrella, the fern Psilotum and PCR-generated phytochrome sequences from a few other ferns. The phytochrome gene of the moss Physcomitretla tumed out not to contain the aberrant C-terminal third of the phytochrome from the moss Ceratodon, but the transmitter module-like sequences fonnd in other phytochromes. A series of differeni phytochrome genes was detected in Psilotum, Differences between the amino acid sequences derived from them ranged from about 5 to more than 22%. Some of these genes are likely pseudogenes. Analysis by phylogenetic tree constructions revealed that higher and lower plant phytocbromes evolved with different velocities. Lower plant phytochromes form a sepai'ote family characterized by a high degree of similarity. The amino acid differences bettt'een phytochrome types detected in a single species of higher plants are about two-fold higher than the differences between phylochromes ' Of species of lower plants belonging to different 'divisions (Physcomitrella and Selaginelki), Future studies on phytochrome sequences may eventually also throw light on the significance of P.'iilotum in the evolution of vascular plants.

show abstract

Chloroplast Genes in Higher Plants

Cited by 3 publications

References 0 publications

Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event

Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event

Phytochrome evolution: Phytochrome genes in ferns and mosses

Phytochrome evolution: Phytochrome genes in ferns and mosses

Contact Info

Product

Resources

About