Chloroplast Ribosomal Protein S7 of Chlamydomonas Binds to Chloroplast mRNA Leader Sequences and May Be Involved in Translation Initiation

Fargo, David C.; Boynton, John E.; Gillham, Nicholas W.

doi:10.1105/tpc.13.1.207

Cited by 29 publications

(13 citation statements)

References 28 publications

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“…Reduced rps7 expression is also likely related to lower growth rates, although this has not been rigorously examined in phytoplankton [53]. Our results suggest that E. huxleyi cells grown under high CO 2 undergo a reduction in translation initiation in some instances [54]. The non-differential expression of histone H2B under different CO 2 conditions may be explained by indications that this protein is involved in processes other than nucleosome packaging since our identification the identified H2B from the E. huxleyi genome (protein ID 96192) also contained a 50S ribosome-binding GTPase (Pfam: CL0023) and a peptidase domain (Pfam: CL0012).…”

Section: Discussionmentioning

confidence: 81%

Responses of the Emiliania huxleyi Proteome to Ocean Acidification

et al. 2013

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Ocean acidification due to rising atmospheric CO2 is expected to affect the physiology of important calcifying marine organisms, but the nature and magnitude of change is yet to be established. In coccolithophores, different species and strains display varying calcification responses to ocean acidification, but the underlying biochemical properties remain unknown. We employed an approach combining tandem mass-spectrometry with isobaric tagging (iTRAQ) and multiple database searching to identify proteins that were differentially expressed in cells of the marine coccolithophore species Emiliania huxleyi (strain NZEH) between two CO2 conditions: 395 (∼current day) and ∼1340 p.p.m.v. CO2. Cells exposed to the higher CO2 condition contained more cellular particulate inorganic carbon (CaCO3) and particulate organic nitrogen and carbon than those maintained in present-day conditions. These results are linked with the observation that cells grew slower under elevated CO2, indicating cell cycle disruption. Under high CO2 conditions, coccospheres were larger and cells possessed bigger coccoliths that did not show any signs of malformation compared to those from cells grown under present-day CO2 levels. No differences in calcification rate, particulate organic carbon production or cellular organic carbon: nitrogen ratios were observed. Results were not related to nutrient limitation or acclimation status of cells. At least 46 homologous protein groups from a variety of functional processes were quantified in these experiments, of which four (histones H2A, H3, H4 and a chloroplastic 30S ribosomal protein S7) showed down-regulation in all replicates exposed to high CO2, perhaps reflecting the decrease in growth rate. We present evidence of cellular stress responses but proteins associated with many key metabolic processes remained unaltered. Our results therefore suggest that this E. huxleyi strain possesses some acclimation mechanisms to tolerate future CO2 scenarios, although the observed decline in growth rate may be an overriding factor affecting the success of this ecotype in future oceans.

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Section: Discussionmentioning

confidence: 81%

Responses of the Emiliania huxleyi Proteome to Ocean Acidification

et al. 2013

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“…In a previous study [29], rpl23 was reported to be essential for plants and loss of rpl36 result in severe morphological aberrations, low translational efficiency, poor photoautotrophic growth [29]. As an rRNA binding protein, rps7 plays a role in the regulation of chloroplast translation [30]. However, the experimental research on the specific function of rps7 is limited at present.…”

Section: Resultsmentioning

confidence: 99%

Complete chloroplast genomes of 14 mangroves: phylogenetic and genomic comparative analyses

Shi

Han²,

Li³

et al. 2019

Preprint

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AbstractMangroves are main components of an ecosystem which connect land and ocean and is of significant ecological importance. They are found around the world and taxonomically distributed in 17 families. Until now there has been no evolutionary phylogenetic analyses on mangroves based on complete plastome sequences. In order to infer the relationship between mangroves and terrestrial plants at the molecular level, we generated chloroplast genomes of 14 mangrove species from eight families, spanning six orders: Fabales (Pongamia pinnata), Lamiales (Avicennia marina), Malpighiales (Excoecaria agallocha, Bruguiera sexangula, Kandelia obovata, Rhizophora stylosa, Ceriops tagal), Malvales (Hibiscus tiliaceus, Heritiera littoralis, Thespesia populnea), Myrtales (Laguncularia racemose, Sonneratia ovata, Pemphis acidula), and Sapindales (Xylocarpus moluccensis). The whole-genome length of these chloroplasts is from 149kb to 168kb. They have a conserved structure, with two Inverted Repeat (IRa and IRb, ~25.8kb), a large single-copy region (LSC, ~89.0kb), a short single-copy (SSC, ~18.9kb) region, as well as ~130 genes (85 protein-coding, 37 tRNA, and 8 rRNA). The number of simple sequence repeats (SSRs) varied between mangrove species. Phylogenetic analysis using complete chloroplast genomes of 71 mangrove and land plants, confirmed the previously reported phylogeny within rosids, including the positioning of obscure families such as Linaceae within Malpighiales. Most mangrove chloroplast genes are conserved and we found six genes subjected to positive or neutral selection. Genomic comparison showed IR regions have lower divergence than other regions. Our study firstly reported several plastid genetic resource for mangroves, and the determined evolutionary locations as well as comparative analyses of these species provid insights into the mangrove genetic and phylogenetic research.

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“…We hypothesized that compromised ribosomes may be responsible for the MSC16 phenotype; however, proteomic analysis did not show significant differences in protein production between MSC16 and wild-type B (Del Valle-Echevarria et al 2015). It is possible that translation of certain mitochondrial transcripts is dependent on the RPS7 protein (Fargo et al 2001) and results in the MSC phenotype (Del Valle-Echevarria et al 2015). …”

Section: Discussionmentioning

confidence: 99%

Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber

et al. 2016

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Key message Pentatricopeptide repeat (PPR) 336 was identified as the candidate gene for Paternal Sorting of Mitochondria (Psm), a nuclear locus that affects the predominant mitochondria transmitted to progenies. Cucumber (Cucumis sativus L.) is a useful plant to study organellar-nuclear interactions because its organelles show differential transmission, maternal for chloroplasts and paternal for mitochondria. The mitochondrial DNA (mtDNA) of cucumber is relatively large due in part to accumulation of repetitive DNAs and recombination among these repetitive regions produces structurally polymorphic mtDNAs associated with paternally transmitted mosaic (MSC) phenotypes. The mitochondrial mutant MSC16 possesses an under-representation of ribosomal protein S7 (rps7), a key component of the small ribosomal subunit in the mitochondrion. A nuclear locus, Paternal Sorting of Mitochondria (Psm), affects the predominant mitochondria transmitted to progenies generated from crosses with MSC16 as the male parent. Using single nucleotide polymorphisms, Psm was mapped to a 170 kb region on chromosome 3 of cucumber and pentatricopeptide repeat (PPR) 336 was identified as the likely candidate gene. PPR336 stabilizes mitochondrial ribosomes in Arabidopsis thaliana and because MSC16 shows reduced transcription of rps7, the cucumber homolog of PPR336 (CsPPR336) as the candidate for Psm is consistent with a nuclear effect on ribosome assembly or stability in the mitochondrion. We used polymorphisms in CsPPR336 to genotype progenies segregating at Psm and recovered only one Psm−/− plant with the MSC phenotype, indicating that the combination of the Psm− allele with mitochondria from MSC16 is almost always lethal. This research illustrates the usefulness of the MSC mutants of cucumber to reveal and study unique interactions between the mitochondrion and nucleus.

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Chloroplast Ribosomal Protein S7 of Chlamydomonas Binds to Chloroplast mRNA Leader Sequences and May Be Involved in Translation Initiation

Cited by 29 publications

References 28 publications

Responses of the Emiliania huxleyi Proteome to Ocean Acidification

Responses of the Emiliania huxleyi Proteome to Ocean Acidification

Complete chloroplast genomes of 14 mangroves: phylogenetic and genomic comparative analyses

Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber

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