2003
DOI: 10.1002/glia.10212
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Chlorotoxin‐sensitive Ca2+‐activated Cl channel in type R2 reactive astrocytes from adult rat brain

Abstract: Astrocytes express four types of Cl(-) or anion channels, but Ca(2+)-activated Cl(-) (Cl(Ca)) channels have not been described. We studied Cl(-) channels in a morphologically distinct subpopulation ( approximately 5% of cells) of small (10-12 micro m, 11.8 +/- 0.6 pF), phase-dark, GFAP-positive native reactive astrocytes (NRAs) freshly isolated from injured adult rat brains. Their resting potential, -57.1 +/- 4.0 mV, polarized to -72.7 +/- 4.5 mV with BAPTA-AM, an intracellular Ca(2+) chelator, and depolarized… Show more

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Cited by 18 publications
(15 citation statements)
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“…Of note, unlike the block by NPPB, which develops within seconds, complete block by Cltx requires 10-15 minutes, and the example illustrated is recorded 15 minutes after application of 1 μM Cltx. Interestingly, a similar, slow time-course of drug action has been reported for Cltx blockade of single Cl − channels recorded from reactive astrocytes (Dalton et al, 2003). This is unlike the effects of scorpion peptides on other ion channels, which typically occur within milliseconds (Possani et al, 2000).…”
Section: A Putative CL − Channel Inhibitor (Cltx) Is Currently In Clisupporting
confidence: 53%
“…Of note, unlike the block by NPPB, which develops within seconds, complete block by Cltx requires 10-15 minutes, and the example illustrated is recorded 15 minutes after application of 1 μM Cltx. Interestingly, a similar, slow time-course of drug action has been reported for Cltx blockade of single Cl − channels recorded from reactive astrocytes (Dalton et al, 2003). This is unlike the effects of scorpion peptides on other ion channels, which typically occur within milliseconds (Possani et al, 2000).…”
Section: A Putative CL − Channel Inhibitor (Cltx) Is Currently In Clisupporting
confidence: 53%
“…3 Glioma cells have been reported to express a glioma-specific chloride ion channel that is sensitive to Cltx. 4,5 In in vivo targeting and biodistribution experiments, 125 I-and 1 Department of Nuclear Medicine, The First People's Hospital, Shanghai Jiaotong University, Shanghai, People's Republic of China. 2 Institute of Protein Research, Tongji University, Shanghai, People's Republic of China.…”
Section: Introductionmentioning
confidence: 99%
“…The molecular interaction of Cltx with Cl -channels is not fully understood. A recent study suggests that Cltx binds to and inhibits large conductance Cl -channels in reactive astrocytes (30), however, as is the case in glioma cells (27,31), inhibition of channels required several minutes of exposure to the peptide to develop. This timecourse of block is more suggestive of an indirect interaction of the peptide with the ion channel.…”
Section: Glioma Cell Invasion Requires CL -Channel-mediated Cell Shrimentioning
confidence: 99%