Chocolate industry side streams as a valuable feedstock for microbial long-chain dicarboxylic acid production

Bauwelinck, Jordy; Caluwé, Michel; Wijnants, Marc; Wittner, Nikolett; Broos, Waut; Dries, Jan; Akkermans, Veerle; Tavernier, Serge; Cornet, I.

doi:10.1016/j.bej.2020.107888

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…LCDAs are used in various industries such as in the production of powder coatings and grease, in the cosmetic industry as an emulsifier, waxing agent in lubricants, and they are also used in the production of polyesters and polyamides, making use of their double functionality [12,76]. The global market size of C18 dicarboxylic acid is estimated to reach USD 9.4 billion by 2024 [12].…”

Section: Long-chain Dicarboxylic Acids From Wastewatermentioning

confidence: 99%

“…Moreover, sugar-containing waste/wastewater would be even more economical as this might not require extra glucose for cell growth. Recently, Bauwelinck et al [12] applied a chocolate industry side stream as a feedstock for a commercially available oleaginous yeast named C.tropicalis ATCC20962. They could convert 47.5% of the fats present in chocolate water to LCDA.…”

Section: Long-chain Dicarboxylic Acids From Wastewatermentioning

confidence: 99%

“…For example, volatile fatty acids (VFAs) such as acetic acid can be produced [5], which can act as an intermediate platform chemical for a variety of other chemicals [6,7]. In addition, productions of larger carboxylic acids are known, including medium-chain carboxylic acids (MCCAs) [8][9][10][11] and even long-chain dicarboxylic acids (LCDAs), have recently been reported [12]. Next to the direct production of small acid molecules, also indirect acid production through thermal degradation of intracellular polyhydroxyalkanoates (PHAs) is a possible route, yielding unsaturated fatty acids [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Recovery Techniques Enabling Circular Chemistry from Wastewater

et al. 2022

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In an era where it becomes less and less accepted to just send waste to landfills and release wastewater into the environment without treatment, numerous initiatives are pursued to facilitate chemical production from waste. This includes microbial conversions of waste in digesters, and with this type of approach, a variety of chemicals can be produced. Typical for digestion systems is that the products are present only in (very) dilute amounts. For such productions to be technically and economically interesting to pursue, it is of key importance that effective product recovery strategies are being developed. In this review, we focus on the recovery of biologically produced carboxylic acids, including volatile fatty acids (VFAs), medium-chain carboxylic acids (MCCAs), long-chain dicarboxylic acids (LCDAs) being directly produced by microorganisms, and indirectly produced unsaturated short-chain acids (USCA), as well as polymers. Key recovery techniques for carboxylic acids in solution include liquid-liquid extraction, adsorption, and membrane separations. The route toward USCA is discussed, including their production by thermal treatment of intracellular polyhydroxyalkanoates (PHA) polymers and the downstream separations. Polymers included in this review are extracellular polymeric substances (EPS). Strategies for fractionation of the different fractions of EPS are discussed, aiming at the valorization of both polysaccharides and proteins. It is concluded that several separation strategies have the potential to further develop the wastewater valorization chains.

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Section: Long-chain Dicarboxylic Acids From Wastewatermentioning

confidence: 99%

Section: Long-chain Dicarboxylic Acids From Wastewatermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recovery Techniques Enabling Circular Chemistry from Wastewater

et al. 2022

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“…The extraction was performed on a Yellow line RS orbital shaker at 210 rpm at 50 • C for 20 h. The extract was quantitatively transferred to an evaporation flask, and the solvents were removed by rota-evaporation. The resulting residue was dissolved in 1 mL tetrahydrofuran (THF) and analyzed by GPC as described by Bauwelinck et al [45]. The obtained triglyceride, diglyceride, monoglyceride, and free fatty acid concentrations in THF were converted to the total lipid content by Equation (1).…”

Section: Lipidsmentioning

confidence: 99%

“…Next, the mixture was centrifuged at 8000 rpm, and the organic layer was pipetted off and filtered over a 0.2-µm PTFE filter. The sample was injected into the GC-FID system described by Bauwelinck et al [45].…”

Section: Total Lipid Content (%) =mentioning

confidence: 99%

Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts R. kratochvilovae EXF7516 and C. oleaginosum ATCC 20509

et al. 2022

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During the conversion of lignocellulose, phenolic wastewaters are generated. Therefore, researchers have investigated wastewater valorization processes in which these pollutants are converted to chemicals, i.e., lipids. However, wastewaters are lean feedstocks, so these valorization processes in research typically require the addition of large quantities of sugars and sterilization, which increase costs. This paper investigates a repeated batch fermentation strategy with Rhodotorula kratochvilovae EXF7516 and Cutaneotrichosporon oleaginosum ATCC 20509, without these requirements. The pollutant removal and its conversion to microbial oil were evaluated. Because of the presence of non-monomeric substrates, the ligninolytic enzyme activity was also investigated. The repeated batch fermentation strategy was successful, as more lipids accumulated every cycle, up to a total of 5.4 g/L (23% cell dry weight). In addition, the yeasts consumed up to 87% of monomeric substrates, i.e., sugars, aromatics, and organics acids, and up to 23% of non-monomeric substrates, i.e., partially degraded xylan, lignin, cellulose. Interestingly, lipid production was only observed during the harvest phase of each cycle, as the cells experienced stress, possibly due to oxygen limitation. This work presents the first results on the feasibility of valorizing non-sterilized lignocellulosic wastewater with R. kratochvilovae and C. oleaginosum using a cost-effective repeated batch strategy.

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Adipic Acid

2023

Pathway Design for Industrial Fermentation

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Chocolate industry side streams as a valuable feedstock for microbial long-chain dicarboxylic acid production

Cited by 7 publications

References 27 publications

Recovery Techniques Enabling Circular Chemistry from Wastewater

Recovery Techniques Enabling Circular Chemistry from Wastewater

Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts R. kratochvilovae EXF7516 and C. oleaginosum ATCC 20509

Adipic Acid

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