Choice of experimental venue matters in ecotoxicology studies: Comparison of a laboratory-based and an outdoor mesocosm experiment

Mikó, Zsanett; Ujszegi, János; Gál, Zoltán; Imrei, Zoltán; Hettyey, Attila

doi:10.1016/j.aquatox.2015.07.014

Cited by 37 publications

(28 citation statements)

References 79 publications

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“…However, Johnson et al employed a study design in which all tadpoles experienced intraspecific competition; this additional stressor could have caused temperature variability to interact with chlorothalonil and drive substantial lethal effects. Mikó et al compared the toxicity of glyphosate to Rana dalmatina tadpoles between laboratory and ambient environments in mesocosms in which tadpoles experienced interspecific competition; they determined that ambient conditions reduce toxicity relative to laboratory environments. Because tadpoles in our study did not experience interspecific competition, we would not have detected interactions between temperature and competition, although such study designs may more closely approximate realistic ecological conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Higher temperatures increase the lethality of carbaryl to amphibians . Johnson et al and Mikó et al compared toxicology trials from standard laboratory climate and ambient climate conditions, and Johnson et al found higher toxicity of chlorothalonil to tadpoles in ambient environments, although Miko et al found higher toxicity of glyphosate in laboratory environments. However, neither Johnson et al nor Mikó et al experimentally controlled thermal regimes.…”

Section: Introductionmentioning

confidence: 99%

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Additive effects of mean temperature, temperature variability, and chlorothalonil to red‐eyed treefrog (Agalychnis callidryas)larvae

Alza

Donnelly²,

Whitfield³

2016

Enviro Toxic and Chemistry

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Amphibian populations are declining globally, and multiple anthropogenic stressors, including contamination by pesticides and shifting climates, are driving these declines. Climate change may increase average temperatures or increase temperature variability, either of which may affect the susceptibility of nontarget organisms to contaminants. Eight-day ecotoxicological assays were conducted with red-eyed treefrog (Agalychnis callidryas) larvae to test for additive and interactive effects of exposure to the fungicide chlorothalonil, average temperature, and temperature variability on tadpole growth and survival. Egg masses were collected from seasonal ponds at La Selva Biological Station in Costa Rica, and tadpoles were exposed to a series of chlorothalonil concentrations across a range of ecologically relevant mean temperatures (23.4-27.3 °C) and daily temperature fluctuations (1.1-9.9 °C). Survival was measured each day, and tadpole growth was measured at the end of each trial. Concentrations of chlorothalonil ≥60 µg/L reduced survival, although survival was not affected by mean temperature or daily temperature range, and there were no synergistic interactions between chlorothalonil and temperature regime on survival. Chlorothalonil suppressed tadpole growth at relatively low concentrations (∼15 µg/L). There were impacts of both average temperature and daily temperature range on tadpole growth, although there were no synergistic interactions between temperature regimes and chlorothalonil. The results should inform efforts to manage ecosystems impacted by multiple large-scale anthropogenic stressors as well as methods for the design of ecologically appropriate toxicology trials. Environ Toxicol Chem 2016;35:2998-3004. © 2016 SETAC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Additive effects of mean temperature, temperature variability, and chlorothalonil to red‐eyed treefrog (Agalychnis callidryas)larvae

Alza

Donnelly²,

Whitfield³

2016

Enviro Toxic and Chemistry

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“…However, the complexity in the natural environment is not fully reflected in the relatively small volume of water used in laboratory experiments, and the time scales used are usually short. For example, Miko et al (2015) compared results from ecotoxity tests on tadpoles in the lab and in outdoor mesocos which showed strikingly different results.…”

Section: Introductionmentioning

confidence: 99%

An indoor pelagic mesocosm facility to simulate multiple water-column characteristics

Båmstedt¹,

Larsson²

2018

Int Aquat Res

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Mesocosms are important research tools in aquatic ecology because they close the gap between laboratory studies at the individual or lower organization level and field studies at the population and ecosystem level. However, most mesocosm studies regarding the pelagic environment do not consider the effects of physical factors like water-column stratification, turbulence and mixing. Neglecting such factors might bias the results compared to the natural system. Using a unique indoor mesocosm facility, we present results on how different water-column stratifications can be made and how they act as barriers for exchange between water layers. Turbulent mixing, simulated by vertically rotating incubation vessels, is shown to be of high importance for primary production, generating up to nine times higher production in humus-rich water than incubation vessels at fixed depths. Convective stirring is shown to be an attractive method for generating different turbulence conditions, and different temperature settings can be used to get turnover times from 84 h or more down to 17 min for a 5-m water parcel. We also demonstrate how an anoxic bottom layer can be achieved by stimulating heterotrophic bacteria through addition of bioavailable organic carbon.

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“…Unfortunately, similar studies comparing the effects of pesticides on different test sites are scarce (Mikó et al, 2015).…”

mentioning

confidence: 99%

“…According to Arenzon (2011), to carry out ecotoxicological tests, even standardized ones, there is always a concern to reproduce the effects on the field into the laboratory, and it can be done by new approaches, as in situ tests but also with the use of native species that are more representative of the study sites. Mikó et al (2015) investigated the importance of the experimental site for ecotoxicological studies and demonstrated considerable discrepancies between classic laboratory test results and mesocosms experiments.…”

mentioning

confidence: 99%

Ecotoxicological evaluation of Glyphosate use in controlling E. crassipes e Salvinia sp. macrophytes

Maria¹,

Castro²,

Lange³

et al. 2024

Ecotoxicol. Environ. Contam.

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The present work aimed to carry out traditional ecotoxicological and ecosystemic tests in microcosms for the evaluation of the use of glyphosate herbicide adopted to control floating aquatic macrophytes in aquatic ecosystems. Ecotoxicity tests were performed using standardized methodology and standard species of different trophic levels. Although the active ingredient glyphosate is toxic, the toxicity of its commercial formulation is higher. The inert ingredient of the Roundup Original ® composition may be responsible for the higher commercial compound toxicity. Experiments in microcosms indicated that the use of the formulation is possible, in considering tested concentration and controlling the spray process, without causing toxic effect on the aquatic ecosystem. Complementary studies evidenced the risk of using the product in aquatic environments without vegetation cover, being not recommended the herbicide application in a place without floating aquatic macrophytes cover.

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Choice of experimental venue matters in ecotoxicology studies: Comparison of a laboratory-based and an outdoor mesocosm experiment

Cited by 37 publications

References 79 publications

Additive effects of mean temperature, temperature variability, and chlorothalonil to red‐eyed treefrog (Agalychnis callidryas)larvae

Additive effects of mean temperature, temperature variability, and chlorothalonil to red‐eyed treefrog (Agalychnis callidryas)larvae

An indoor pelagic mesocosm facility to simulate multiple water-column characteristics

Ecotoxicological evaluation of Glyphosate use in controlling E. crassipes e Salvinia sp. macrophytes

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