Choice of Hemostatic Agent Influences Adhesion Formation in a Rat Cecal Adhesion Model

Hoffmann, Nathan E.; Siddiqui, Sameer; Agarwal, Shvetank; McKellar, Stephen H.; Kurtz, H. J.; Gettman, Matthew T.; Ereth, Mark H.

doi:10.1016/j.jss.2008.08.008

Cited by 63 publications

(55 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adhesion formation was subjected to the scoring systems by Lauder et al [20] and Hoffmann et al [23], as shown in tables 1 and 2. Table 4 shows the scores as classified according to the Lauder scoring scheme.…”

Section: Resultsmentioning

confidence: 99%

“…Upon autopsy on postoperative day 7 and repeated laparotomy, the areas of the cecum and abdominal sidewall were evaluated for macroscopic adhesion by 2 independent observers according to scoring schemes by Lauder et al [20] and Hoffmann et al [23], as shown in tables 1 and 2. Photographs of the affected areas were taken from each animal for documentation (20.0-megapixel digital camera, Sony Cyber-shot DSC-RX100).…”

Section: Methodsmentioning

confidence: 99%

“…The extent of adhesion formation was also graded 0-3. These 3 subscores were summed for a total gross adhesion score [23]. …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Poehnert¹,

Abbas

Kreipe

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

Background: Adhesions due to pelvic/abdominal surgery are a common serious pathology possibly entailing severe complications. This study investigates the adhesion prevention capability of the novel starch-based agent 4DryField® PH, which together with saline solution forms a barrier gel. Herein, an optimized adhesion model (OPAM) inducing severe adhesions/agglutinations with high reproducibility was used. Methods: In 19 Lewis rats, a 1 × 2 cm abdominal wall defect was created, the peritoneum of the neighboring cecum was abraded, and both injured areas were approximated by suture. Rats were randomized to control (n = 10) or 4DryField PH treatment (n = 9) groups. Another 8 rats had sham surgery for safety assessment of 4DryField PH. At day 7, the quantity and quality of adhesions were assessed macro-/microscopically and evaluated statistically. Results: 4DryField PH treatment significantly reduced the incidence and severity of adhesions as verified by significantly improved adhesion scorings (0.4 vs. 4.5; 1.1 vs. 9). Histology revealed reconstitution of the cecum and abdominal wall including regeneration of the visceral/parietal peritoneum. In sham-operated rats, 4DryField PH did not induce adhesion formation. Conclusions: 4DryField PH gel was highly effective in preventing adhesions. Histologically, the injured cecum and abdominal wall regenerated well in the presence of 4DryField PH. Considering the severity of OPAM trauma, the potential of 4DryField PH to prevent adhesions can be rated excellent.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Poehnert¹,

Abbas

Kreipe

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…Although no adverse sequelae were noted in this series, Bioglue is not approved for intraperitoneal use and its effect on the abdominal viscera remains unknown. 15 One published series using Bioglue on pancreatic anastamoses did not report significant intra-abdominal complications, 16 but more work is needed to elucidate the role for this adhesive within the peritoneal cavity.…”

Section: Notes Inguinal Hernia Repair: a Canine Modelmentioning

confidence: 99%

Natural Orifice Translumenal Endoscopic Surgery Inguinal Hernia Repair: A Survival Canine Model

Sherwinter

Gupta

Eckstein

2011

Journal of Laparoendoscopic & Advanced Surgical Techniques

View full text Add to dashboard Cite

Introduction: With over 20 million repairs performed worldwide annually, inguinal hernias represent a significant source of disability and loss of productivity. Natural orifice translumenal endoscopic surgery (NOTESÔ), as a potentially less invasive form of surgery may reduce postoperative disability and accelerate return to work. The objective of this study was to assess the safety and short-term effectiveness of transgastric inguinal herniorrhaphy using a biologic mesh in a survival canine model. Materials and Methods: Under general anesthesia with the animal in trendelenburg position, a gastrostomy was created. A 4 · 6 cm acellular dermal implant was deployed endoscopically across the myopectineal orifice, draped over the cord structures, and secured with Bioglue. Following completion of bilateral repairs the animals were survived for 14 days. At the end of the study period, the animals were euthanized and a necropsy performed. Cultures of a random site within the peritoneal cavity and at the site of implant deployment were obtained. In addition, a visual inspection of the peritoneal cavity was performed. Results: All animals thrived postoperatively and did not manifest signs of peritonitis or sepsis at any point. At necropsy accurate placement and adequate myopectineal coverage was confirmed in all subjects. Cultures of a random site within the peritoneal cavity and at the site of implant deployment had no growth. Discussion: This study confirms that NOTES-inguinal herniorrhaphy using a biologic implant can be performed safely. In addition, the transgastric technique provided good short-term myopectineal coverage without infectious sequelae.

show abstract

“…Uterine horns and cecum were identified. To form adhesions the cecum abrasion model was used [10] . Cecums were scrubbed with a sterile sponge on the antimesenteric surface until punctate bleeding and serosal petechiae occurred.…”

Section: The Animals and The Experimental Modelmentioning

confidence: 99%

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

Işık¹,

Şahbaz²,

Aynıoğlu³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan®, Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions graded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III (P<0.001). The inflammation, neovascularization, and fibrosis scores for Group III were statistically significant and considerably lower than those scores for Groups I and II (P<0.001, P<0.001 and P=0.002 respectively). OT, significantly prevented adhesion formation improving wound healing possibly by suppressing adhesion formation with anti-inflammatory and antioxidant properties. OT may be useful in the prevention of PPA in humans.

show abstract

Choice of Hemostatic Agent Influences Adhesion Formation in a Rat Cecal Adhesion Model

Cited by 63 publications

References 12 publications

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Natural Orifice Translumenal Endoscopic Surgery Inguinal Hernia Repair: A Survival Canine Model

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

Contact Info

Product

Resources

About