Cholera Toxin Subunit B Enabled Multifunctional Glioma‐Targeted Drug Delivery

Guan, Juan; Zhang, Zui; Hu, Xuefeng; Yang, Yang; Chai, Zhilan; Liu, Xiaoqin; Liu, Jican; Gao, Bo; Lu, Weiyue; Qian, Jun; Zhan, Changyou

doi:10.1002/adhm.201700709

Cited by 29 publications

(20 citation statements)

References 42 publications

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“…The orthotropic glioma model was established by implantation of U87 cells in brains of nude mice 62 . Briefly, male nude mice (18–20 g) were anesthetized with chloral hydrate and U87 cells (8 × 10 5 cells suspended in 5 μL PBS) were implanted into the right brain (0.6-mm anterior, 1.8-mm lateral to the bregma with 3-mm depth) using a stereotactic apparatus (Stoteling).…”

Section: Methodsmentioning

confidence: 99%

Brain-targeted drug delivery by manipulating protein corona functions

et al. 2019

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Protein corona presents a major obstacle to bench-to-bedside translation of targeted drug delivery systems, severely affecting targeting yields and directing unfavorable biodistribution. Corona-mediated targeting provides a new impetus for specific drug delivery by precisely manipulating interaction modes of functional plasma proteins on nano-surface. Here bio-inspired liposomes (SP-sLip) were developed by modifying liposomal surface with a short nontoxic peptide derived from Aβ 1-42 that specifically interacts with the lipid-binding domain of exchangeable apolipoproteins. SP-sLip absorb plasma apolipoproteins A1, E and J, consequently exposing receptor-binding domain of apolipoproteins to achieve brain-targeted delivery. Doxorubicin loaded SP-sLip (SP-sLip/DOX) show significant enhancement of brain distribution and anti-brain cancer effect in comparison to doxorubicin loaded plain liposomes. SP-sLip preserve functions of the absorbed human plasma ApoE, and the corona-mediated targeting strategy works in SP modified PLGA nanoparticles. The present study may pave a new avenue to facilitate clinical translation of targeted drug delivery systems.

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Section: Methodsmentioning

confidence: 99%

Brain-targeted drug delivery by manipulating protein corona functions

et al. 2019

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“…Blood was sampled and mice were killed at 1, 4, 12, and 24 h after injection. Liver and spleen were collected, weighed and DiI was extracted using methanol 25 . After the tissues were weighed and transferred into a 2 mL tube, 0.5 mL triton-X 100 (with a percentage of 5% in water) was added and the tissues were smashed by an ultrasonic pulverizer.…”

Section: Methodsmentioning

confidence: 99%

Enhanced immunocompatibility of ligand-targeted liposomes by attenuating natural IgM absorption

et al. 2018

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Targeting ligands are anticipated to facilitate the precise delivery of therapeutic agents to diseased tissues; however, they may also severely affect the interaction of nanocarriers with plasma proteins. Here, we study the immunocompatibility of brain-targeted liposomes, which inversely correlates with absorbed natural IgM. Modification of long, stable positively charged peptide ligands on liposomes is inclined to absorb natural IgM, leading to rapid clearance and enhanced immunogenicity. Small peptidomimetic D8 developed by computer-aided peptide design exhibits improved immunocompatibility by attenuating natural IgM absorption. The present study highlights the effects of peptide ligands on the formed protein corona and in vivo fate of liposomes. Stable positively charged peptide ligands play double-edged roles in targeted delivery, preserving in vivo bioactivities for binding receptors and long-term unfavorable interactions with the innate immune system. The development of D8 provides insights into how to rationally design immunocompatible drug delivery systems by modulating the protein corona composition.

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“…For example, some studies focus on the relationship between particle properties like surface chemistry and protein corona formation 14,23,[59][60][61][62][63][64][65] and others analyze the protein corona as particle properties (like size or shape) needed to interpret cellular or biological effects. 26,38,[66][67][68][69][70][71][72] Further aspects include improving particle properties to change protein corona composition and biological response; [73][74][75] targeting or characterizing of particles for usage as drug carriers or for other medical purposes; [76][77][78][79][80][81] or the comparison of different particles. 60,68,82 Some studies report on the topic of biological function by single-protein measurement or functionality analysis.…”

Section: Application Of Separation Methods In Corona Analyticsmentioning

confidence: 99%