Cholestasis induced antinociception and decreased gene expression of MOR1 in rat brain

Ahmadi, Shamseddin; Karami, Z.; Mohammadian, Ali; Khosrobakhsh, Farnoosh; Rostamzadeh, Jalal

doi:10.1016/j.neuroscience.2014.08.063

Cited by 18 publications

(16 citation statements)

References 35 publications

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“…It has been shown that plasma total opioid levels in cholestatic liver disease is increased, which may underlie changes including pruritus and analgesia after BDL (Alemi et al 2013;Bergasa et al 1995;Nicoll et al 2005). In support of the involvement of a opioid system in the antinociception induced by BDL, we have recently reported that it was almost completely prevented by injection of a muopioid receptor antagonist, naloxone, on day 21 of BDL (Ahmadi et al 2015). In addition, NMDA receptor pathway has been shown to be involved in modulation of cholestasisinduced antinociception in rats (Hasanein et al 2007).…”

Section: Days After Bdlmentioning

confidence: 68%

“…The results of a recent research from our laboratory revealed that the mu-opioid receptor 1 (MOR1) gene expression was also influenced by BDL in the PFC, hippocampus and hypothalamus but not in the striatum (Ahmadi et al 2015). We proposed that the observed changes in the MOR1 gene expression might directly or indirectly result from an increase in endogenous opioids.…”

Section: Days After Bdlmentioning

confidence: 93%

“…In the third set, three groups of control, sham and BDL (n=4, in each group) were used to examine the GluN1 gene expression in the PFC, hippocampus and striatum. These animals were independent of those that we used in our previous study (Ahmadi et al 2015).…”

Section: Experimental Designmentioning

confidence: 99%

“…We used the second set of animals including control, sham and BDL groups to assess pain behavior using a hotplate test according to the method that we have earlier reported (Ahmadi et al 2015). In brief, time elapse between placement of each animal on the hotplate (52±0.1°C) and licking one of the hind paws or first jumping was measured as an index of pain reaction latency.…”

Section: Measurement Of Nociceptionmentioning

confidence: 99%

“…Total RNA was extracted from 70 mg of each tissue using a TRIZOL method according to our previous study (Ahmadi et al 2015). In brief, each tissue sample homogenized for 60 s in a tube containing 1 ml lysis buffer (RNX+ reagent, Cinagen, Tehran, Iran), with a high speed homogenizer (Silent Crusher S., Heidolph, Germany), then was subjected to a total RNA extraction according to manufacturer's manual (Cinagen, Tehran, Iran).…”

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

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Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

2015

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We investigate changes in gene expression of GluN1 subunit of N-Methyl-D-Aspartate (NMDA) receptor in the prefrontal cortex (PFC), hippocampus and striatum in a rat model of hepatic encephalopathy (HE). We used male Wistar rats in which HE was induced after a common bile duct ligation (BDL). The animals were divided into three sets, and each set included three groups of control, sham operated and BDL. In the first set of animals, blood samples collected for biochemical analysis on day 21 of BDL. In the second set, changes in nociception threshold was assessed on day 21 of BDL using a hotplate test. In the third set, whole brain extracted, and the PFC, the hippocampus and the striatum in each rat were immediately dissected. We used a semi-quantitative RT-PCR method for evaluating the GluN1 gene expression. The biochemical analyses showed that plasma levels of ammonia and bilirubin in BDL rats were significantly increased compared to the sham control group on day 21 of BDL (P < 0.01). Nociception threshold was also increased in rats with BDL compared to sham group (P < 0.001). The results revealed that the GluN1 gene expression at mRNA levels in BDL group was decreased by 19 % in the PFC (P < 0.05) but increased by 82 % in the hippocampus (P < 0.01) compared to the sham control group; however, no significant change was observed in the striatum. It can be concluded that HE affects the GluN1 gene expression in rat brain with a site-specific pattern, and the PFC and hippocampus are more sensitive areas than striatum.

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Section: Days After Bdlmentioning

confidence: 68%

Section: Days After Bdlmentioning

confidence: 93%

Section: Experimental Designmentioning

confidence: 99%

Section: Measurement Of Nociceptionmentioning

confidence: 99%

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

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Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

2015

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Anxiety in rats with bile duct ligation is associated with activation of JNK3 mitogen-activated protein kinase in the hippocampus

Ahmadi

Khaledi

2020

Metab Brain Dis

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Altered Expression of Differential Genes in Thoracic Spinal Cord Involved in Experimental Cholestatic Itch Mouse Model

Chen

Wang

et al. 2018

CURR MED SCI

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The spinal origin of cholestatic itch in experimental obstructive jaundice mouse model remains poorly understood. In this study, the jaundice model was established by bile duct ligation (BDL) in mice, and differential gene expression patterns were analyzed in the lower thoracic spinal cord involved in cholestatic pruritus after BDL operation using high-throughput RNA sequencing. At 21st day after BDL, the expression levels of ENSRNOG00000060523, ENSRNOG00000058405 and ENSRNOG00000055193 mRNA were significantly up-regulated, and those of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOGOOOOOO19607, ENSRNOG00000020647, ENSRNOG00000046289, Gemin8, Serpina3n and Trim63 mRNA were significantly down-regulated in BDL group. The RNAseq data of selected mRNAs were validated by RT-qPCR. The expression levels of ENSRNOG00000042197, ENSRNOG00000008478, ENSRNOGOOOOOO 19607, ENSRNOG00000020647, ENSRNOG00000046289 and Serpina3n mRNA were significantly down-regulated in BDL group. This study suggested that cholestatic pruritus in experimental obstructive jaundice mouse model is related with in the changes of gene expression profiles in spinal cord.

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Cholestasis induced antinociception and decreased gene expression of MOR1 in rat brain

Cited by 18 publications

References 35 publications

Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

Anxiety in rats with bile duct ligation is associated with activation of JNK3 mitogen-activated protein kinase in the hippocampus

Altered Expression of Differential Genes in Thoracic Spinal Cord Involved in Experimental Cholestatic Itch Mouse Model

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