Cholesterol Binding to Simple Micelles in Aqueous Bile-Salt–Cholesterol Solutions

Gallstone formation analysis using the particle appearance, the particle binding to calcium ions, and the cholesterol nucleation with time in supersaturated taurocholate–lecithin–calcium ion solutions

“…[19] For TC and TC-L biles, the polydispersity of the dominant particles was~30-40%. [6,23,30] In general, the aggregates with diameters between 2 and 10 nm have been confirmed to be micelles while between 50 nm and 300 nm referred to be vesicles. [14][15][16][17]19] As for the aggregates larger than 400 nm, they could be inferred to as aggregated or fused vesicles.…”

“…For simple TC micelles, the CMC and n S have been estimated to be~6-7 mM and~5, respectively. [23,24,30] Based on Equation (1), [TC 1 ] of 6 mM, n S of 5, and the onset of simple micelle concentration of~1 mM, the K SM value could be reasonably estimated to bẽ 1.286 × 10 −4 mM −4 . Furthermore, according to Equation (1), the estimated K SM value and simple TC micelle concentration of~10 mM, the corresponding concentration of TC monomers could be estimated to be~9.5 mM.…”

“…Moreover, based on the particle size difference using quasielastic lightscattering (QLS) measurements, the vesicle or micelle appearance can be detected. [5,6] Further analysis of the Ch molecules released from simple micelles and bound to mixed micelles was carried out [7,19,30] to reasonably interpret the influence of ChA T and vesicle appearance on the time-dependent nucleation results in three TC to L ratio biles in the presence of calcium ions. Noticeably, the experimental results in the low TC to L ratio system were compared with previous nucleation results in the low TCDC to L ratio system.…”

Estimation of the Taurocholate to Lecithin Molar Ratio of Mixed Micelles in Taurocholate‐Lecithin Solutions by an Interaction Model and Laser Light‐Scattering Measurements

“…A previous study showed that, for simple TC micelles, the values of CSM and nS,TC are 10 mM and 5, respectively. 23 To investigate the change of [L 1 ] with different L levels, the NMTC concentration should be assumed a constant larger than 25 mM. When the NMTC was maintained at 25 mM in solutions with three L levels of 6 mM, 12 mM, and 21 mM, according to Eq.…”

“…[9][10][11] Moreover, the total aggregation number (~10) in simple TC micelles estimated by GP-HPLC was out of the range (5 ± 2) reported in the literature. 21,23 Therefore, the accuracy of the K values estimated by GP-HPLC is questionable, and better techniques for investigating the true K values of mixed TC-L micelles coexisting with simple TC micelles are needed.…”