Cholesterol-conjugated supramolecular assemblies of low generations polyamidoamine dendrimers for enhanced EGFP plasmid DNA transfection

Redox-sensitive, cholesterol-bearing PEGylated poly(propylene imine)-based dendrimersomes for drug and gene delivery to cancer cells

“…of the DNA at all tested ratios for the low-cholesterol dendrimer, and at dendrimer: DNA weight ratios of 1:1 and higher for the high-cholesterol dendrimer, which displayed less primary amines at its periphery than its low-cholesterol counterpart ( Figures 7A and B). DNA condensation occurred instantly and was found to be stable for at least 24 h. DNA condensing ability of these modified dendrimers was of the same range to that reported for generations 1, 2 and 3-PAMAM dendrimers conjugated to 40% cholesterol, [30][31] PEGylated poly(amidoamine) dendron-bearing lipids-based lipoplexes, 16 octadecyl alkyl chain-bearing generations 2-and 3-PAMAM dendrons forming lipoplexes following probe sonication 46 (up to 100% for all of these formulations).…”

“…The CAC values for the low and high cholesterol-modified PEGylated dendrimers were found to be close to that obtained with generation 1-, generation 2-and generation 3-PAMAM dendrimer conjugated to cholesterol at 40% mole ratio of cholesterol/primary amine (12.5 ± 3.2 µg/mL for generation 2-PAMAM-cholesterol, 28.9 ± 4.1 µg/mL for generation 1-PAMAM-cholesterol and 28.7 ± 3.5 µg/mL for generation 3-PAMAMcholesterol). 30 They were lower than the CMC observed when using generation-3 PAMAM dendrimers conjugated to cholesterol at 6% and 15% mole ratio of cholesterol/primary amine (respectively 86.6 ± 5.6 µg/mL and 50.2 ± 5.1 µg/mL). 31 These low CMC facilitated the incorporation of the PAMAM-cholesterol in the PEG-stabilized liposome bilayer.…”

“…Overall, dendriplexes showed a smaller hydrodynamic diameter due to the presence of the highly hydrophobic cholesterol in the backbone, leading to the formation of more compact dendriplexes in comparison to not only C18 alkyl chain-modified generation 3-PAMAM dendrimers, but also cholesterol-modified generations 1, 2 and 3-PAMAM-based dendriplexes. 16,[30][31] Both low-cholesterol and high-cholesterol dendriplexes presented an overall average size lower than the unmodified DAB dendriplex, which had an average size of 196 nm (polydispersity index: 0.683). 8 This smaller size will further facilitate their extravasation to cancer cells.…”

Harnessing self-assembled peptide nanoparticles in epitope vaccine design

“…These nanoparticles encapsulate antigens within their cores and then deliver them to the target cells. Lipidation leads to the formation of amphiphiles, which improve diffusion across epithelial barriers and provide stability against degradation agents (Golkar et al, 2016a(Golkar et al, , 2016bZhao et al, 2014). These structures target vaccines to DCs and stimulate the activation of these cells through self-adjuvant properties.…”

Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma:in vitroandin vivoevaluation

“…Either cholesteryl chloroformate or alkyl-PEG was conjugated to PAMAM or alkyl-PAMAM as described previously with some modifications [22,23]. For grafting of 5 and 3% of primary amines of PAMAMs with either cholesterol or alkyl-PEG,10 mg of PAMAM or alkyl-PAMAM dissolved in 3 ml chloroform and cholesteryl chloroformate (0.6 mg) and/or alkyl-PEG (4.97 mg) in 1 ml chloroform was slowly added to PAMAM solutions over 5-10 min.…”