“…The intraphagosomal pH of all strains started to decline within minutes, and at 150 min after infection wild-type phagosomes were modestly acidified with a mean pH of 6.4, while phagosomes containing heat-inactivated wild-type bacteria were acidified to pH 5.8. These levels of intraphagosomal pH for live and dead mycobacteria are consistent with data reported elsewhere (de Chastellier & Thilo, 2006;Russell et al, 2005;Sturgill-Koszycki et al, 1994). The pH of the lspA : : aph phagosomes was 6.3, which is not significantly different from that of M. tuberculosis wild-type phago- Values represent the mean±SD (error bars) of four (resting BMDM) and three (activated BMDM) independent experiments.…”