Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels

Nakajima, Katsuyuki; Saito, Toshimitsu; Tamura, Aki; Suzuki, Manami; Nakano, Takamitsu; Adachi, Masakazu; Tanaka, Akira; Tada, Norio; Nakamura, Haruo; Campos, Elisa; Havel, Richard J.

doi:10.1016/0009-8981(93)90062-9

Cited by 406 publications

(312 citation statements)

References 32 publications

Supporting

Mentioning

300

Contrasting

Unclassified

Order By: Relevance

“…It is unclear whythere was no significant difference in the levels of RLP-C between groups S and N in the hypertriglyceridemic patient subgroup. RLP-C has been reported to be highly correlated with the plasma triglyceride level (9). In the hypertriglyceridemic patient subgroup, RLP-Clevels were relatively high even in group N, which is perhaps affected by higher TG levels ( Table 4).…”

Section: Discussionmentioning

confidence: 92%

“…RLP-Cis the level of this subpopulation quantified by the assay of cholesterol. A more detailed characterization has been given elsewhere (9).…”

Section: Discussionmentioning

confidence: 99%

“…Although there are some reports on the levels of VLDLremnants or intermediate-density lipoproteins (IDL) in patients with coronary artery stenosis (22), reports on the levels of RLP-C in patients with coronary artery stenosis are few (9,23). Associations of RLP-Clevels with coronary events have recently been reported (24,25).…”

Section: Discussionmentioning

confidence: 99%

“…Wealso evaluated the levels of remnant-like particle cholesterol (RLP-C). RLP-Cis a recently established simple assay method that estimates at least a part of serum triglyceride-rich lipoprotein remnants (9).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Predictive Value of Remnant-like Particle Cholesterol as an Indicator of Coronary Artery Stenosis in Patients with Normal Serum Triglyceride Levels.

et al. 2000

View full text Add to dashboard Cite

Objective Wedesigned the present study to evaluate the association of various lipid and fibrinolytic components with coronary artery stenosis with respect to the triglyceride (TG) level.Methods Levels of TG, remnant-like particle cholesterol (RLP-C), total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), lipoprotein-(a), uric acid, blood glucose, tissue plasminogen activator (t-PA), t-PA inhibitor type 1, antithrombin III, and protein C were measured in 208 patients who underwent diagnostic coronary angiograms.Patients Of these 208 patients, 59 were hypertriglyceridemic (150 mg/dl or higher) and 149 were normotriglyceridemic.Results Both LDL-Cand HDL-Cshowed significant differences between patients with and those without coronary artery stenosis in both hypertriglyceridemic and normotriglyceridemic patient subgroups. However, RLP-C showeda significant difference in the normotriglyceridemic patient subgroup (p=0.012) but not in the hypertriglyceridemic patient subgroup (p=0.736). Conclusion Our current retrospective study disclosed that RLP-Clevels are closely associated with coronary artery stenosis in patients with normal TGlevels.

show abstract

Section: Discussionmentioning

confidence: 92%

“…RLP-Cis the level of this subpopulation quantified by the assay of cholesterol. A more detailed characterization has been given elsewhere (9).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Predictive Value of Remnant-like Particle Cholesterol as an Indicator of Coronary Artery Stenosis in Patients with Normal Serum Triglyceride Levels.

et al. 2000

View full text Add to dashboard Cite

show abstract

“…23 The RLP fraction was prepared by use of an immune-separation technique described by Campos et al 8 and Nakajima and colleagues. 9 Briefly, 5 L of serum was added to 300 L of mixed immunoaffinity gel suspension containing monoclonal anti-human apoA-I (H-12) and anti-human apoB100 (JI-H) antibodies (Immunoresearch Laboratories). The reaction mixture was gently shaken for 120 minutes at room temperature.…”

Section: Biochemical Analysismentioning

confidence: 99%

Elevated Remnant-Like Particles in Heterozygous Familial Hypercholesterolemia and Response to Statin Therapy

et al. 2002

View full text Add to dashboard Cite

Background-Remnant lipoproteins (RLP-C) are considered important in atherogenesis. Hence, this study was designed to assess RLP-C levels and the effect of statin therapy in patients with familial hypercholesterolemia (FH). Elevated RLP-C levels have been associated with the presence and progression of atherosclerotic disease, and their presence in FH patients has been proposed but never established in a large cohort, nor has their response to statin therapy been confirmed. Methods and Results-FH patients were recruited from 36 lipid clinics. After a washout period of 6 weeks, all patients were started on monotherapy with 80 mg of simvastatin for 2 years. RLP-C levels were assessed by an immuneseparation assay. In 327 FH patients, RLP-C measurements could be performed before and after treatment. Mean total cholesterol (10.55Ϯ2.17 mmol/L), mean LDL cholesterol (8.40Ϯ2.13 mmol/L), and median RLP-C (0.47 mmol/L) levels were all severely elevated at baseline. After treatment, RLP-C levels were reduced by 49% (0.24 mmol/L; PϽ0.0001). Even patients with normal triglyceride levels had elevated RLP-C levels at baseline, and those with high RLP-C levels were generally characterized by a very atherogenic lipoprotein profile. Conclusions-Baseline RLP-C levels are severely elevated in FH patients and are reduced by simvastatin but do not return to normal. These elevated RLP-C levels could be the consequence of impaired function of the LDL receptor in FH. RLP-C levels in FH contribute to an atherogenic lipoprotein profile and could identify patients who require additional treatment. (Circulation. 2002;106:788-792.)

show abstract

Emerging Noninvasive Biochemical Measures to Predict Cardiovascular Risk

Pahor¹,

Elam²,

Garrison³

et al. 1999

Arch Intern Med

View full text Add to dashboard Cite

Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels

Cited by 406 publications

References 32 publications

Predictive Value of Remnant-like Particle Cholesterol as an Indicator of Coronary Artery Stenosis in Patients with Normal Serum Triglyceride Levels.

Predictive Value of Remnant-like Particle Cholesterol as an Indicator of Coronary Artery Stenosis in Patients with Normal Serum Triglyceride Levels.

Elevated Remnant-Like Particles in Heterozygous Familial Hypercholesterolemia and Response to Statin Therapy

Emerging Noninvasive Biochemical Measures to Predict Cardiovascular Risk

Contact Info

Product

Resources

About