Cholesterol-Linked Fluorescent Molecular Beacons with Enhanced Cell Permeability

Seo, Young Jun; Jeong, Hoon Eui; Bang, Eun‐Kyoung; Hwang, Gil Tae; Jung, Jong Ha; Jang, Sung Key; Kim, Byeang Hyean

doi:10.1021/bc060078q

Cited by 32 publications

(15 citation statements)

References 24 publications

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“…1) (16), however, demonstration of their formation in living cells remains elusive. To investigate whether TERRA RNA G-quadruplexes exist in living cells, we employed a light-switching pyrene probe that has been designed to employ the capability of pyrene to form fluorescent excited-state dimers (excimers) (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36). The advantage of the distance dependence of excimer formation with pyrene can be used as a unique excimer signaling device for detecting G-quadruplex structure ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Telomeric repeat-containing RNA structure in living cells

Suzuki

Ito

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

166

138

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Telomeric repeat-containing RNA (referred to as TERRA), a noncoding RNA molecule, has recently been found in mammalian cells. The detailed structural features and function of the TERRA RNA at human chromosome ends remain unclear, although this RNA molecule may be a key component of the telomere machinery. In the present studies, we investigated the structural features of human TERRA RNA in living cells. Using a light-switching pyrene probe, we found that human TERRA RNA forms a parallel G-quadruplex structure in living cells, providing the in vivo evidence for the presence of the G-quadruplex in human TERRA RNA. Furthermore, imaging experiments clearly show that TERRA RNA G-quadruplex localizes to telomere DNA at cell nuclei. These results provide valuable information to allow understanding of the structure and function of human TERRA RNA.telomere biology | telomere RNA | RNA folding | chemical probe

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Section: Resultsmentioning

confidence: 99%

Telomeric repeat-containing RNA structure in living cells

Suzuki

Ito

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

166

138

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“…To this aim, lipid moieties have been coupled to molecules to be delivered inside the cells. For example, a cholesterol unit has been attached to a free terminus of an oligonucleotide hairpin, thus enhancing its cellular delivery in comparison to conventional transfection methods [61]. In another study, cholesterol-derivatized oligonucleotides also showed a rapid binding and cytosolic partition in cells [62].…”

Section: Cpps and Cell Entrymentioning

confidence: 99%

Cell-penetrating and cell-targeting peptides in drug delivery

Vivès

Schmidt

Pèlegrin

2008

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

317

272

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During the last decade, the potential of peptides for drug delivery into cells has been highlighted by the discovery of several cell-penetrating peptides (CPPs). CPPs are very efficient in delivering various molecules into cells. However, except in some specific cases, their lack of cell specificity remains the major drawback for their clinical development. At the same time, various peptides with specific binding activity for a given cell line (cell-targeting peptides) have also been reported in the literature. One of the goals of the next years will be to optimize the tissue and cell delivery of therapeutic molecules by means of peptides which combine both targeting and internalization advantages. In this review, we describe the main strategies that are currently in use or likely to be employed in the near future to associate both targeting and delivery properties.

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“…The practical utility of the free ends in EMS MBs of Figure 8.19c has been demonstrated by attaching a cholesterol moiety at the 5 -end of the ODN. The cellular permeability of the cholesterol-appended ODN was enhanced significantly as compared to that of free ODN [122]. Some fluorophores form nonradiative dimers when they are in close proximity.…”

Section: Quencher-free Molecular Beaconmentioning

confidence: 97%