Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability

Batissaco, Leonardo; Arruda, Rubens Paes de; Alves, Maíra Bianchi Rodrigues; Torres, Mariana Andrade; Lemes, Kleber Menegon; Prado-Filho, Roberto Romano; Almeida, Tamie Guibu de; Andrade, André Furugen Cesar de; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.repbio.2020.01.002

Cited by 18 publications

(11 citation statements)

References 39 publications

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“…This treatment improves sperm membrane stability after incorporating exogenous cholesterol to the plasma membrane, which in turn enhances sperm cryosurvival, motility, mitochondrial activity and the number of sperm attached to zona pellucida, reducing at the same time cryo-capacitation and premature tyrosine phosphorylation [133][134][135][136]. The beneficial effects of CLC seem to be greater in those ejaculates with low freezability, at least in ram sperm [137]. Moreover, the addition of CLC to the extender attenuated in gazelle sperm the degradation of three proteins related to energy metabolism and cytoskeletal organization (CAPZB, HSP90A, PAGM2) during the freezing-thawing process compared to untreated sperm, which may explain the increased motility observed in CLC treated sperm [38].…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

152

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

152

118

View full text Add to dashboard Cite

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“…Para avaliar a integridade da membrana plasmática e do potencial acrossômico e de membrana mitocondrial, as sondas fluorescentes utilizadas foram: iodeto de propídio (PI), aglutinina Pisum sativum conjugada com isotiocianato de fluoresceína (FITC-PSA) e 5,5',6,6'tetracloro-1,1, iodeto de 3,3'tetraetilbenzimidazolilcarbocianina (JC-1), respectivamente (adaptado Pavaneli et al, 2020) (17) . Para a análise da peroxidação lipídica foram utilizados 4,4-Difluoro-5-(4-fenil-1,3-butadienil)-4-bora-3a, 4a-diaza-sindaceno ácido-3-undecanoico (C11-BODIPY581 / 591) (18) e dihidroetídio (DHE) (19) . Essas análises foram realizadas usando uma citometria de fluxo (BD Accuri™ C6).…”

Section: Análises Pós Criopreservaçãounclassified

Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros

et al. 2023

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Resumo Em busca de melhorias na criopreservação do sêmen, substâncias naturais são comumente estudadas com o objetivo de melhorar a qualidade do sêmen. O objetivo deste estudo foi avaliar o efeito da adição de sucos de laranja, abacaxi e beterraba em diferentes concentrações e combinações ao diluidor de criopreservação de sêmen ovino. Foram utilizados cinco ejaculados de cinco carneiros adultos. O pool de sêmen foi diluído em diluente à base de gema de ovo e misturado com os seguintes 15 tratamentos (na concentração final de 400x10⁶ sptz/ml): laranja 10% (O10) e 15% (O15); abacaxi 10% (P10) e 15% (P15); beterraba 10% (B10) e 15% (B15); abacaxi + laranja 10% (PO10) e 15% (PO15); abacaxi + beterraba 10% (PB10) e 15% (PB15); beterraba + laranja 10% (BO10) e 15% (BO15); abacaxi + beterraba + laranja 10% (PBO10) e 15% (PBO15); e o grupo controle (CON). Pós-descongelação em palhetas de 0,25 ml a análise da qualidade do sêmen criopreservado foi realizada pelo CASA e citometria de fluxo. A análise de variância foi realizada e as médias comparadas pelo teste SNK. O teste de correlação de Pearson também foi realizado. Nenhum efeito foi observado na adição de sucos ao diluidor de sêmen antes da criopreservação. Após o descongelamento, embora estatisticamente semelhante ao grupo controle, a motilidade total do grupo B10 atingiu padrões aceitáveis de motilidade total. Além disso, o grupo B10 apresentou os maiores valores (p<0,05) de motilidade progressiva que o grupo controle ou os outros tratamentos. A adição de 10% de suco de beterraba ao diluente de sêmen ovino pode melhorar a criopreservação da motilidade espermática.

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“…To evaluate integrity of plasma membrane and acrosome and mitochondrial membrane potential, the fluorescent probes used were: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) and 5.5',6.6'-tetrachloro-1,1,3,3'tetraethylbenzimidazolylcarbocyanine iodide (JC-1), respectively (adapted from Pavaneli et al, 2020) (17) . For the analysis of lipid peroxidation, we used 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a, 4a-diaza-sindacene acid-3-undecanoic (C11-BODIPY581/591) (18) and dihydroethidium (DHE) (19) . These analyses were performed using a flow cytometer (BD Accuri™ C6).…”

Section: Post-cryopreservation Semen Analysismentioning

confidence: 99%

Addition of orange, pineapple and beet juices as extenders for cryopreservation of ram semen

et al. 2023

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Searching for improvements in semen cryopreservation, natural substances are commonly studied focusing to improve the sperm quality. The aim of this study were evaluated the effect of adding orange, pineapple, and beet juices in different concentrations and combinations to the ram semen cryopreservation extender. Five ejaculates from five adult rams were used. The semen pool was diluted in egg yolk-based extender and mixed with the following 15 treatments (at a final concentration of 400.106 sptz/mL): orange 10% (O10) and 15% (O15); pineapple 10% (P10) and 15% (P15); beet 10% (B10) and 15% (B15); pineapple + orange 10% (PO10) and 15% (PO15); pineapple + beet 10% (PB10) and 15% (PB15); beet + orange 10% (BO10) and 15% (BO15); pineapple + beet + orange 10% (PBO10) and 15% (PBO15); and the control group (CON). Post-thaw in 0.25 mL straws semen quality analysis of cryopreserved semen was performed by CASA and flow cytometry. Analysis of variance (PROC GLM) was carried out and the averages were compared using the SNK test. Pearson's correlation test was also performed. No effect was noted in the addition of juices to the semen extender prior to cryopreservation. Post-thawed, although, statistically similar to the control group, the total motility of the B10 group reached acceptable standards of total motility. In addition, B10 group showed the highest values (p<0.05) of progressive motility than control group or other treatments. The addition of 10% beet juice to the ram semen extender can improve the cryopreservation of sperm motility.

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Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability

Cited by 18 publications

References 39 publications

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros

Addition of orange, pineapple and beet juices as extenders for cryopreservation of ram semen

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