Cholinergic activation of settlement inCiona intestinalis metamorphosing larvae

Coniglio, L; Morale, A; Angelini, Cristiano; Falugi, Carla

doi:10.1002/(sici)1097-010x(19980301)280:4<314::aid-jez5>3.0.co;2-r

Cited by 30 publications

(23 citation statements)

References 7 publications

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“…It is thought that metamorphosis in cnidarians is induced, at least in part, by the release of neuropeptides that spread through the larvae following the stimulation of sensory cells by external cues (Müller & Leitz 2002). AChE appears to play an integral role in the metamorphosis of some marine larvae, such as ascidians, where AChE activity is sometimes localised on the surface of adhesive papillae prior to settlement (Coniglio et al 1998). Cholinesterase activity was not confirmed in adult A. millepora branches in this study.…”

Section: Discussioncontrasting

confidence: 65%

Insecticides and a fungicide affect multiple coral life stages

Markey¹,

Baird²,

Humphrey³

et al. 2007

Mar. Ecol. Prog. Ser.

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Section: Discussioncontrasting

confidence: 65%

Insecticides and a fungicide affect multiple coral life stages

Markey¹,

Baird²,

Humphrey³

et al. 2007

Mar. Ecol. Prog. Ser.

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“…In order to test which specific carbohydrates are important in reducing IL-8 levels, bacterial strains were treated with three different lectins. Succinyl-ConA and ConA have affinity for terminal α-D-glucosyl and α-Dmannosyl residues [21] . Tetragonolobus purpureas (TPA) on the other hand as an affinity for α-L-fucosyl residues and an especially high affinity for α-L-fucose residues on type Ⅱ chain blood group oligosaccharides [22] .…”

Section: Results From Chymotrypsin and Trypsin Digested Bacterialmentioning

confidence: 99%

Infant intestinal Enterococcus faecalis down-regulates infl ammatory responses in human intestinal cell lines

Wang¹,

Ng²,

Chow³

et al. 2008

WJG

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AIM:To investigate the ability of Lactic acid bacteria (LAB) to modulate inflammatory reaction in human intestinal cell lines (Caco-2, HT-29 and HCT116). Different strains of LAB isolated from new born infants and fermented milk, together with the strains obtained from culture collections were tested. METHODS:LABs were treated with human intestinal cell lines. ELISA was used to detect IL-8 and TGF-β protein secretion. Cytokines and Toll like receptors (TLRs) gene expression were assessed using RT-PCR. Conditional medium, sonicated bacteria and UV killed bacteria were used to find the effecter molecules on the bacteria. Carbohydrate oxidation and protein digestion were applied to figure out the molecules' residues. Adhesion assays were further carried out. RESULTS: It was found that Enterococcus faecalisis the main immune modulator among the LABs by downregulation of IL-8 secretion and upregulation of TGF-β. Strikingly, the effect was only observed in four strains of E. faecalis out of the 27 isolated and tested.

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“…The ability of the neurotransmitter acetylcholine to induce settlement and metamorphosis has been shown to have highly variable results across marine invertebrate taxa (Coniglio 1998;Dobretsov and Qian 2003;Yu et al 2007Yu et al , 2008Yu et al , 2010bYoung 2009). L-Phenylalanine, L-Tyrosine and dopamine are members of the epinephrine biosynthesis pathway (Fig.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms

2011

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Please cite this article as: Alfaro, Andrea C., Young, Tim, Ganesan, Annapoorna Maitrayee, Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms, Aquaculture (2011), doi: 10.1016/j.aquaculture.2011.08.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Pseudoalteromonas sp. AMGP1 were tested for their abilities to induce larval settlement. Toxicity effects of each treatment also were simultaneously identified by recording larval mortalities. Results indicate that all chemicals used induced larvae to settle, with acetylcholine being the most effective (~24% at 10 -6 M compared to <2% in control assays). Toxicities of treatment compounds were low at optimal settlement inducing concentrations, except for L-Tryptophan (~32%) and GABA (~59%). Our data suggest that catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. While serotonin precursors did induce low levels of larval settlement at some concentrations, high toxicity responses to 5-HTP at 10 -5 M, combined with complete settlement inhibition indicate A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTthat the mechanism of action may be more complex than can be elucidated in this study. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases across all three strains, and settlement inhibition was observed when larvae were exposed to biofilm exudates of all bacterial strains. Comparisons of A. maoriana responses to other endemic and worldwide distributed mussel species are provided as a means to highlight potential evolutionary differences in chemoreception mechanisms. Highlights 1.Mussel larval settlement responses were tested with neuroactive compounds amino acids, and bacterial biofilms.2. All chemicals induced larvae to settle, with acetylcholine being the most effective.3. Catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. Settlement responses to serotonin precursors require further investigationsince there were high toxicity responses.5. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases, and exposure to biofilm exudates resulted in settlement inhibition.

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Cholinergic activation of settlement inCiona intestinalis metamorphosing larvae

Cited by 30 publications

References 7 publications

Insecticides and a fungicide affect multiple coral life stages

Insecticides and a fungicide affect multiple coral life stages

Infant intestinal Enterococcus faecalis down-regulates infl ammatory responses in human intestinal cell lines

Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms

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