Cholinergic Control of Catecholamine Release in the Eel

Alkharrat, H; Weiß, Ursula; Tran, Quynh; Nibbio, Barbara; Scholz, Stefan; Epple, August

doi:10.1006/gcen.1997.6954

Cited by 15 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with prior studies conducted in several teleosts including rainbow trout, American eel, Atlantic cod and the common carp (Nilsson et al 1976, Fritsche et al 1993, Reid & Perry 1995, Al-Kharrat et al 1997, Gfell et al 1997, Montpetit & Perry 1999, the carbacholevoked elevation of adrenaline and noradrenaline secretion was significantly reduced in the presence of hexamethonium (nicotinic receptor antagonist) and atropine (muscarinic receptor antagonists). Because cholinoceptor blockade did not affect ckVIP-or hPACAP-27-evoked adrenaline secretion, it was clear that the effects of the neuropeptides on trout chromaffin cells occur independently of cholinergic stimulation.…”

Section: Direct Effects Of Exogenous Vip and Pacap On In Situ Catechosupporting

confidence: 91%

Vasoactive intestinal polypeptide- and pituitary adenylate cyclase activating polypeptide-mediated control of catecholamine release from chromaffin tissue in the rainbow trout, Oncorhynchus mykiss

Montpetit

Perry

2000

Journal of Endocrinology

View full text Add to dashboard Cite

The aim of the present investigation was to assess the relative contributions of cholinergic (acetylcholine) and non-cholinergic vasoactive intestinal polypeptide (VIP), and pituitary adenylate cyclase activating polypeptide (PACAP) neurotransmitters in the neuronal control of catecholamine secretion from the chromaffin tissue lining the posterior cardinal vein of the rainbow trout (Oncorhynchus mykiss). Using an in situ saline-perfused posterior cardinal vein preparation, it was demonstrated that exogenous administration of chicken VIP or human PACAP-27 caused a dose-dependent increase in adrenaline secretion; noradrenaline secretion was unaffected. Analysis of dose-response curves indicated that VIP and PACAP stimulated the secretion of adrenaline with a similar degree of potency (ED 50 for VIP=1·90 10 11 mol/kg; ED 50 for PACAP=1·03 10 11 mol/kg). The VIP/PACAP-elicited secretion was diminished in the presence of the VIP receptor antagonist, VIP 6-28, but was unaffected by the PACAP receptor antagonist, PACAP 6-27, or the cholinergic antagonists, hexamethonium and atropine. Thus, this is the first study to demonstrate a direct stimulatory role for VIP or PACAP in catecholamine secretion from piscine chromaffin cells.The relative contribution of cholinergic and noncholinergic neurotransmitters in the neuronal control of catecholamine secretion from the chromaffin tissue was evaluated using an in situ nerve-stimulating technique previously validated by us in the rainbow trout. This was accomplished by comparing catecholamine secretion in the presence or absence of cholinergic and the VIP and PACAP receptor antagonists during different levels of electrical stimulation. The results demonstrated that cholinergic stimulation predominated during high frequency of electrical stimulation (20 Hz) while the noncholinergic component prevailed at low frequency (1 Hz). Overall, the results of the present investigation demonstrate that VIP and/or PACAP may directly stimulate adrenaline secretion from trout chromaffin cells at low levels of neuronal activity. Therefore, the neuronal control of catecholamine secretion in teleosts may not be confined to cholinergic-evoked events.

show abstract

Section: Direct Effects Of Exogenous Vip and Pacap On In Situ Catechosupporting

confidence: 91%

Vasoactive intestinal polypeptide- and pituitary adenylate cyclase activating polypeptide-mediated control of catecholamine release from chromaffin tissue in the rainbow trout, Oncorhynchus mykiss

Montpetit

Perry

2000

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…The effect of d-FF on A but not NA lends support to the idea that A and not NA stimulates glucose release. The possible differential regulation of the release of A and NA is totally consistent with the fact that catecholamines are stored in different populations of chromaffin cells in fish (Gallo and Civinini, 2003) and that regulation of their release is also different in these animals (Reid et al, 1994;Bernier and Perry, 1996;Al-Kharrat et al, 1997).…”

Section: Discussionsupporting

confidence: 64%

Homeostasis of glucose in the rainbow trout (Oncorhynchus mykiss Walbaum): the role of serotonin

Tubío

Maceira

Aldegunde

2010

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Yet, the fact that different secretagogues can preferentially induce the secretion of a particular catecholamine implies an ability to specifically stimulate subpopulations of chromaffin cells. For example, administration of cholinergic agonists to in situ preparations causes the release of adrenaline and noradrenaline (Nilsson et al, 1976;Opdyke et al, 1983;Al-Kharrat et al, 1997;Gfell et al, 1997;Montpetit and Perry, 1999;Montpetit et al, 2001). By contrast, while the administration of angiotensin II elicits predominantly the secretion of adrenaline in fish (Bernier and Perry, 1997;Bernier et al, 1999), injection of natriuretic peptide in dogfish (Squalus acanthias) causes the release of noradrenaline only (McKendry et al, 1999;Montpetit et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In fish, the secretory profile of catecholamines during cholinergic stimulation of chromaffin tissue features the release of both adrenaline and noradrenaline (Nilsson et al, 1976;Fritsche et al, 1993;Reid and Perry, 1995;Al-Kharrat et al, 1997;Gfell et al, 1997;Montpetit and Perry, 1999). By contrast, however, administration of a range of VIP and PACAP doses selectively causes the release of adrenaline from the chromaffin cells of all vertebrate species studied to date (Guo and Wakade, 1994;Montpetit and Perry, 2000).…”

mentioning

confidence: 99%

Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss)

Montpetit

Shahsavarani

Perry

2003

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY The current model for the neuronal control of catecholamine release from piscine chromaffin cells advocates that the neurotransmitters vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are co-released with acetylcholine from preganglionic fibres upon nerve stimulation. Both VIP and PACAP elicit the secretion of exclusively adrenaline from rainbow trout chromaffin cells, which presumably arises from the activation of VPAC type receptors. Thus, the goals of the present study were (1) to localise VPAC receptors in the chromaffin cell fraction of the posterior cardinal vein (PCV) of trout and (2) to test the hypothesis that the selective secretion of adrenaline elicited by VIP could be explained by the absence of the VPAC receptors from the noradrenaline-containing cells. Fluorescent labelling of chromaffin cells using aldehyde-induced fluorescence of catecholamines and antisera raised against dopamineβ-hydroxylase (DβH) revealed a distinct layer of chromaffin cells lining the walls of the PCV. Furthermore, specific VIP-binding sites were demonstrated on chromaffin cells using a biotinylated VIP that was previously established as being bioactive. Although multiple labelling experiments revealed that a number of DβH-positive cells were immunonegative for phenylethanolamine N-methyl transferase (PNMT;noradrenaline-containing cells versus adrenaline-containing cells,respectively), labelling of VIP-binding sites was similar to that of DβH labelling, suggesting that all chromaffin cells possess VIP-binding sites. Pharmacological assessment of the VIP-binding sites indicated that they exhibited characteristics of VPAC receptors. Specifically, the labelling of VIP-binding sites was prevented after pre-treatment of PCV tissue sections with unlabelled VIP, PACAP or the specific VPAC receptor antagonist VIP 6-28. By contrast, sections pre-treated with the PAC1 receptor blocker PACAP 6-27 displayed normal labelling of VIP-binding sites. Finally, partial cDNA clones for the trout VPAC1 and VPAC2 receptor were obtained and sequenced. Tissue distribution experiments using RT-PCR revealed the presence of VPAC1 receptor mRNA but not that of the VPAC2 receptor in the PCV tissue. The results provide direct evidence that VIP and PACAP can elicit the secretion of adrenaline from the chromaffin tissue via specific VIP-binding sites that exhibit properties of VPAC receptors. However, the selective secretion of adrenaline by VIP or PACAP cannot be explained by a lack of VIP-binding sites on the noradrenaline-containing cells.

show abstract

Cholinergic Control of Catecholamine Release in the Eel

Cited by 15 publications

References 18 publications

Vasoactive intestinal polypeptide- and pituitary adenylate cyclase activating polypeptide-mediated control of catecholamine release from chromaffin tissue in the rainbow trout, Oncorhynchus mykiss

Vasoactive intestinal polypeptide- and pituitary adenylate cyclase activating polypeptide-mediated control of catecholamine release from chromaffin tissue in the rainbow trout, Oncorhynchus mykiss

Homeostasis of glucose in the rainbow trout (Oncorhynchus mykiss Walbaum): the role of serotonin

Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss)

Contact Info

Product

Resources

About