Cholinesterase activity and effects of its inhibition by neurotoxic drugs in Dictyostelium discoideum

Falugi, Carla; Amaroli, Andrea; Evangelisti, V.; Viarengo, Aldo; Corrado, Maria Umberta Delmonte

doi:10.1016/s0045-6535(02)00143-1

Cited by 31 publications

(19 citation statements)

References 20 publications

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“…The amoebae aggregate and migrate towards an extracellular cyclic AMP signalling source by chemotaxis, giving rise to the first multicellular stage, which undergoes the process of culmination leading to the formation of the fruiting body (Loomis, 1975;Escalante & Vicente 2000). In D. discoideum, we citochemically and spectrophotochemically detected ChE activities able to cleave the substrate PrChI with high affinity and the substrate AcTChI, with less affinity (Falugi et al, 2002). The non-denaturing electrophoresis pattern showed that the PrChI substrate was hydrolysed by a single enzyme activity, while the AcTChI susbstrate was hydrolysed by two enzyme activities, one migrating like the 260-kDa molecular form of Electrophorus electricus AChE and the other corresponding to the molecular form revealed by the PrTChI substrate (Falugi et al, 2002).…”

Section: Basic Research Into the Effects Of Pesticides On Dictyostelimentioning

confidence: 95%

“…In D. discoideum, we citochemically and spectrophotochemically detected ChE activities able to cleave the substrate PrChI with high affinity and the substrate AcTChI, with less affinity (Falugi et al, 2002). The non-denaturing electrophoresis pattern showed that the PrChI substrate was hydrolysed by a single enzyme activity, while the AcTChI susbstrate was hydrolysed by two enzyme activities, one migrating like the 260-kDa molecular form of Electrophorus electricus AChE and the other corresponding to the molecular form revealed by the PrTChI substrate (Falugi et al, 2002). To characterise the PrChE, we spectrophotometrically evaluated the effects of pH, temperature variations and specific inhibitors.…”

Section: Basic Research Into the Effects Of Pesticides On Dictyostelimentioning

confidence: 99%

“…The OPs employed were diazinon and cidial, which caused an inhibition of 67% and 52% respectively at a concentration of 10 -6 M, whereas the CAs were carbaryl and pirimicarb, which caused an inhibition of 63% and 61% at the same concentration as the OPs (Falugi et al, 2002). In addition, a dose-dependent inhibition of PrChE activity was found for diazinon concentrations ranging from 10 -7 M to 10 -3 M. …”

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confidence: 93%

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The Effects of Pesticides on Dictyostelium Cholinesterase, from Basic to Applied Research

Amaroli¹

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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Section: Basic Research Into the Effects Of Pesticides On Dictyostelimentioning

confidence: 95%

Section: Basic Research Into the Effects Of Pesticides On Dictyostelimentioning

confidence: 99%

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confidence: 93%

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The Effects of Pesticides on Dictyostelium Cholinesterase, from Basic to Applied Research

Amaroli¹

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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“…The presence and localization of the ChE activity was investigated by incubating the Control, Micro(g) and Rec samples overnight at 4°C with the acetyl-β-methyl thiocholine iodide (AcTChI) substrate according to the Karnovsky and Roots method, 24 modified by Falugi et al 25 Samples were then washed in 0.1 M PBS, pH 7.4. Nuclei were counterstained with DAPI (1:10,000 in PBS for 5 min).…”

Section: Cytochemical Procedures To Detect the Cholinesterase Activitymentioning

confidence: 99%

“…ChE-like molecules were separated on a 8% polyacrylamide running gel. Electrophoresis was carried out at 12 mA, for 16 h at 5°C, according to the procedure described by Falugi et al 25 The ChE activity was detected with the Karnovsky and Roots method, 24 using AcTChI as substrate. Electrophorus electricus AChE (Sigma-Aldrich) was used as positive control.…”

Section: Non-denaturing Electrophoresis To Detect the Cholinesterase mentioning

confidence: 99%

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

et al. 2018

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Compounds known as chemical mediators, including acetylcholine, have been found not only in animals and humans, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10 -6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.

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Effects of a 50 Hz magnetic field onDictyostelium discoideum (Protista)

Amaroli

Trielli

Bianco

et al. 2006

Bioelectromagnetics

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Some studies have demonstrated that a few biological systems are affected by weak, extremely low frequency (ELF) electromagnetic fields (EMFs), lower than 10 mT. However, to date there is scanty evidence of this effect on Protists in the literature. Due to their peculiarity as single-cell eukaryotic organisms, Protists respond directly to environmental stimuli, thus appearing as very suitable experimental systems. Recently, we showed the presence of propionylcholinesterase (PrChE) activity in single-cell amoebae of Dictyostelium discoideum. This enzyme activity was assumed to be involved in cell-cell and cell-environment interactions, as its inhibition affects cell aggregation and differentiation. In this work, we have exposed single-cell amoebae of D. discoideum to an ELF-EMF of about 200 microT, 50 Hz, for 3 h or 24 h at 21 degrees C. A delay in the early phase of the differentiation was observed in 3 h exposed cells, and a significant decrease in the fission rate appeared in 24 h exposed cells. The PrChE activity was significantly lower in 3 h exposed cells than in the controls, whereas 24 h exposed cells exhibited an increase in this enzyme activity. However, such effects appeared to be transient, as the fission rate and PrChE activity values returned to the respective control values after a 24 h stay under standard conditions.

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Cholinesterase activity and effects of its inhibition by neurotoxic drugs in Dictyostelium discoideum

Cited by 31 publications

References 20 publications

The Effects of Pesticides on Dictyostelium Cholinesterase, from Basic to Applied Research

The Effects of Pesticides on Dictyostelium Cholinesterase, from Basic to Applied Research

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

Effects of a 50 Hz magnetic field onDictyostelium discoideum (Protista)

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