Cholinesterase activity as potential biomarker in two bivalves

Mora, Pascal; Michel, Xavier; Narbonne, Jean‐François

doi:10.1016/s1382-6689(99)00019-8

Cited by 115 publications

(50 citation statements)

References 22 publications

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“…Consistent GST activity has been reported for different body sections (head, pereon, pleon, urosome, pereopods, antennae) in the amphipods Pallasea cancelloides, Eulimnogammarus verrucoso, and Gammarus lacustris [17], while in Macrobrachium malcolmsonii clear differences were observed in GST activity between hepatopancreas and gills [20]. Cholinesterase activity was shown to depend on the tissue analyzed in C. fluminea (with higher activity in the mantle compared to other body tissues) [21] and in M. malcolmsonii where clear differences were observed between brain, hepatopancreas, gills, and muscle [20]. Finally, Chang et al [22] documented that Macrobrachium rosenbergii exposed to trichlorfon inhibited AChE in the hemolymph and hepatopancreas but not in muscle tissue.…”

Section: Inter-and Intra-specific Variationssupporting

confidence: 74%

“…Inhibition of ChE following exposure to OP and carbamate pesticides has been observed for the mollusks: Amblema plicata, Biomphalaria glabrata, Elliptio complanata, Pomaceae patula, and C. fluminea [21,27,29,[46][47][48]. Nevertheless, some studies report lack of effect or irregular response of mollusks exposed to OPs and carbamates [21,27].…”

Section: Cholinesterasementioning

confidence: 99%

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Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

130

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Abstract-Studies investigating the use of biomarkers in pesticide risk assessment have greatly increased in recent years; however, issues concerning the ecological meaning of enzymatic responses have proved controversial. Ideally a good biomarker response should be modulated by the environmental contaminants alone and demonstrate a predictable behavior towards certain types of toxins. As these premises are rarely observed, the present study aims to outline research that has contributed to an understanding of the behavior of two widely used biomarkers, cholinesterase and glutathione-S-transferase, describing environmental and biotic factors that affect their response in freshwater invertebrates. Studies were performed in the main classes of aquatic invertebrates with these biomarkers and conclusions were reached concerning their behavior towards the main classes of pesticides. Links between biomarker responses and conventional endpoints were evaluated so that ecological relevance could be attributed to enzymatic responses. Toxicity of mixtures was investigated, and cases of synergism and antagonism were pointed out as factors changing the expected toxicity of aquatic systems and leading to misinterpretations of biomarker responses. Finally, the use of biomarkers as a tool for biomonitoring and in situ assays was investigated, with discussion of advantages and disadvantages of their use.

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Section: Inter-and Intra-specific Variationssupporting

confidence: 74%

Section: Cholinesterasementioning

confidence: 99%

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

130

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“…Bivalves such as clams, oysters and mussels are the most common indicators of aquatic pollution (Boening 1997), and their cholinesterase activity has been suggested as biomarker of pesticide pollution (Cajaraville et al 2000, Damiens et al 2004). This seems to agree with the values reported in other studies such as Bocquené et al (1997) and Mora et al (1999) who reported high activities in oysters and mussels collected in low polluted areas. These results seem to indicate a higher degree of pollution in Nayarit coastal waters during the dry season, while the values determined in the rainy season are close to those reported by Nadji et al (2010) and Ochoa et al (2013) in clams and oysters challenged with OP pesticides or transplanted to heavily polluted areas.…”

Section: Discussionsupporting

confidence: 93%

CHOLINESTERASE ACTIVITY IN Crassostrea sp. OF NAYARIT (NW MEXICO) COASTAL WATERS

Bautista-Covarrubias

Villaseñor

Bueno

et al. 2017

Rev. Int. Contam. Ambie.

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Cholinesterases (ChE) have been suggested as biomarkers of the presence of organophosphorus insecticides (OP). The activities of these enzymes were determined in abductor muscle, gills and labial palps of oysters collected during the rainy and the dry seasons at eight points of the coastal zone of Nayarit state (NW Mexico), aiming to determine if their variability is related to geographic and seasonal differences in the use of OP. At all stations, ChE mean activity was lower in the dry than in the rainy season, and the muscle had consistently lower values than gills and labial palps. Lower activities were found in Bahía de Banderas, in the southern part of the state, than in the northern stations. This may be explained by the year-round use of OP insecticides in the southern part of the state, whereas herbicides are the most common pesticides used in agricultural areas draining into the northern stations during the rainy season.Palabras clave: esterasas, diferencias en tejidos, diferencias estacionales, diferencias geográficas, insecticidas organofosforados RESUMENLas colinesterasas (ChE) se han propuesto como biomarcadores de la presencia de plaguicidas organofosforados (OF). Se determinó la actividad de estas enzimas en el músculo aductor, las branquias y los palpos labiales de ostras recolectadas durante los periodos de lluvias y estiaje en ocho sitios de muestreo ubicados en la zona costera del estado de Nayarit (noroeste de México), con la finalidad de determinar si su variabilidad está relacionada con diferencias geográficas o estacionales en el uso de OF. En todas las estaciones, la actividad de ChE resultó menor en estiaje que durante el periodo de Rev. Int. Contam. Ambie. 33 (2) 215-220, 2017 DOI: 10.20937/RICA.2017 J.C. Bautista-Covarrubias et al. 216lluvias, y los valores encontrados en el músculo fueron constantemente menores que en las branquias y los palpos labiales. La actividad de ChE fue menor en los organismos recolectados en Bahía de Banderas, en la parte sur del estado, que en las estaciones del norte, probablemente debido al uso de OF durante todo el año en el sur, mientras que los herbicidas son los plaguicidas más usados en las áreas agrícolas que descargan sus efluentes en las áreas de muestreo de la parte norte.

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“…These results indicated that the enzyme present in this species is a cholinesterase that cleaves PrSCh more efficiently than the other investigated substrates. Most studies in bivalves have found a high rate of hydrolysis of AcSCh by an AChE, mainly in gill extracts (Monserrat et al 2002;Mora et al 1999;Valbonesi et al 2003), but in other mollusks, such as Corbicula fluminea and Anodonta woodiana, the ChEs present also use PrSCh as their main substrate in several tissues (Corsi et al 2007;Mora et al 1999). PSA was found to be the preferentially hydrolyzed substrate for CaE, and our results indicated a V max of 78.55 ± 3.27 U/mg protein (Fig.…”

Section: Resultssupporting

confidence: 53%

“…However, several studies have shown great variation in the types of these enzymes and their substrate preferences, tissue distribution and physiological roles among different mollusk species (Mora et al 1999;Stefano et al 2008;Valbonesi et al 2003). The role of acetylcholinesterases (Meng and Liu 2010) in vertebrates as the main hydrolase in the neuromuscular system is well established.…”

mentioning

confidence: 99%

Bivalve Esterases as Biomarker: Identification and Characterization in European Cockles (Cerastoderma edule)

Nilin

Monteiro

Domingues

et al. 2012

Bull Environ Contam Toxicol

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This study characterized esterase activity in Cerastoderma edule tissues using different substrates and specific inhibitors and identified the tissue distribution of esterases in this species. Synthetic thiocholines and thioacetate esters and specific inhibitors (eserine, BW284C51 and iso-OMPA) were used to identify and quantify cholines and carboxyl esterases. The results demonstrated the presence of a non-specific propionyl thiocholine (PrSCh)-cleaving cholinesterase (ChE) and a large amount of carboxylesterases (CaE). For further studies using C. edule esterases as biomarkers, our results suggest that the adductor muscle, with PrSCh (5 mM) as substrate should be used to analyze ChE, and for CaE analyses, phenyl thioacetate should be used in digestive gland extracts (PSA, 5 mM).

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Cholinesterase activity as potential biomarker in two bivalves

Cited by 115 publications

References 22 publications

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

CHOLINESTERASE ACTIVITY IN Crassostrea sp. OF NAYARIT (NW MEXICO) COASTAL WATERS

Bivalve Esterases as Biomarker: Identification and Characterization in European Cockles (Cerastoderma edule)

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