Cholinesterase-based biosensors

Štěpánková, Šárka; Vorčáková, Katarína

doi:10.1080/14756366.2016.1204609

Cited by 36 publications

(18 citation statements)

References 195 publications

(217 reference statements)

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“…In recent years, the increasing request of sensing devices for OPs compound has seen a plethora of new approaches being explored by different research groups . An interesting overview of recent advancements in the techniques used for organophosphate detection and corresponding detection limits has been recently reported by Kumar et al The biosensors mainly based on enzymatic inhibition of acetyl‐cholinesterase or immnunochemistry seem to be the most efficient in term of sensitivity (because they can reach detection limits of 0.1 pM for paraoxon detection). However, most of these sensors present some drawbacks that are peculiar of protein‐based systems, such as long measuring times and limitations because of pH, temperature and solvents.…”

Section: Introductionmentioning

confidence: 99%

Selective detection of organophosphate through molecularly imprinted GERS‐active hybrid organic–inorganic materials

Carboni

Jiang

Malfatti

et al. 2017

J Raman Spectroscopy

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A selective sensing platform for the organophosphate paraoxon, a highly toxic organic pollutant, has been designed and tested on water samples. A porous hybrid organic-inorganic film, synthesised using tetraethoxysilane, 1,8-bis(triethoxysilyl) octane and cetyltrimethylammonium bromide, has been molecularly imprinted with a structural analogue of paraoxon, the diethyl(4-nitrobenzyl)phosphonate, to induce selective recognition. Exfoliated graphene has been incorporated into the porous matrix to provide enhancement of the Raman scattering signal. The Raman sensor has been tested on different concentrations of paraoxon in both ethanol and water/ethanol mixture. The molecular selectivity has been assessed by comparing the Raman signal enhancement of paraoxon with a similar organophosphate, the bis-(4-nitrophenyl) phosphate. The molecularly imprinted film has shown a fourfold increase of the paraoxon signal, when compared with the corresponding not-imprinted. The evaluation of the density of molecular cavities into the molecularly imprinted samples (4.50 * 10 À10 μmol μm À3) has allowed assuming that each molecular cavity is capable of providing a remarkable signal enhancement of 1.47 * 10 12 count * μmol À1 only when recognising paraoxon. The material design has allowed coupling the sensitivity of the graphene-mediated enhancement of Raman scattering with the selectivity of molecular imprinting into a single and potentially portable, analytical system.

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Section: Introductionmentioning

confidence: 99%

Selective detection of organophosphate through molecularly imprinted GERS‐active hybrid organic–inorganic materials

Carboni

Jiang

Malfatti

et al. 2017

J Raman Spectroscopy

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“…The toxicity mechanism of the neurotoxic insecticides is based on the inhibition of acetylcholinesterase (AChE; EC 3.1.1.7) [ 15 ], and the reproduction of this inhibition in vitro can be used for multianalyte selective monitoring. There are reported to be numerous biosensors developed for the detection of neurotoxic organophosphorus and carbamate insecticides based on the inhibition of cholinesterase that were reviewed concerning their general aspects [ 16 ], the parameters influencing the enzymatic inhibition such as the effect of substrate concentration, enzyme total activity or the presence of organic solvents [ 17 ], strategies for biosensor construction using various immobilization methods and the roles of various matrices used [ 18 ], improvement of the selectivity and sensitivity using genetically engineered mutant enzymes [ 19 ], biosensor integration in flow analytical manifolds [ 20 , 21 ], use of special detection techniques such as piezoelectric quartz crystal microbalance [ 22 ], combination with various kinds of nanomaterials [ 23 ], impact of cutting-edge technologies [ 24 ], specific application for fast screening of food samples [ 25 ], etc. Most biosensors were based on electrochemical detection [ 26 ], while optical [ 27 , 28 , 29 , 30 ] and piezoelectric detection [ 22 ] were more rarely explored.…”

Section: Detection Of Neurotoxic Insecticides Based On Cholinestermentioning

confidence: 99%

Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more “biologically relevant” than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides.

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“…A transducer is another key component which can significantly determine the usefulness of a biosensor. Based on the type of the transducer, various ChE-based biosensors can be classified as potentiometric, amperometric, conductometric, photoelectrochemical, optical, and piezoelectric [34].…”

Section: Cholinesterase-based Biosensorsmentioning

confidence: 99%

Cholinesterases and Engineered Mutants for the Detection of Organophosphorus Pesticide Residues

Ndikuryayo

et al. 2018

Sensors

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Nowadays, pesticide residues constitute an increasing public health concern. Cholinesterases, acetylcholinesterase, and butyrylcholinesterase, are reported to be involved in detoxification processes owing to their capability of scavenging organophosphates and carbamates. Thus, these enzymes are targeted for the discovery of sensors aiming at detecting pesticide residues. In recent years, cholinesterase-based biosensors have attracted more and more attention in the detection of pesticides. Herein, this review describes the recent progress on the engineering of cholinesterases and the development of the corresponding sensors that could be used for the detection of organophosphorus pesticide residues.

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Cholinesterase-based biosensors

Cited by 36 publications

References 195 publications

Selective detection of organophosphate through molecularly imprinted GERS‐active hybrid organic–inorganic materials

Selective detection of organophosphate through molecularly imprinted GERS‐active hybrid organic–inorganic materials

Advances in Enzyme-Based Biosensors for Pesticide Detection

Cholinesterases and Engineered Mutants for the Detection of Organophosphorus Pesticide Residues

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