Cholinesterase inhibition and impacts on behavior of the Asian clam, Corbicula fluminea, after exposure to an organophosphate insecticide

Cooper, Naomi L.; Bidwell, Joseph R.

doi:10.1016/j.aquatox.2005.09.012

Cited by 102 publications

(37 citation statements)

References 38 publications

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“…Bivalve siphons play important functional roles in nutritional physiology, defense, and reproduction (Moulton et al, 1996) and are therefore a signal of general health or stress. In fact, previous studies have demonstrated that bivalve siphoning can be influenced by metals or chlorpyrifos (Doherty et al, 1987;Milam and Farris, 1998;Cooper and Bidwell, 2006). Cooper and Bidwell (2006) reported that the valves of the clams were always closed during exposure to 3.13 mg/L chlorpyrifos treatments.…”

Section: Discussionmentioning

confidence: 99%

“…The siphoning rate was measured using a previously described method (Cooper and Bidwell, 2006) with slight modifications and is based on depletion of neutral red dye particles from the water due to filtration by the clams. Immediately after CBZ exposure, five C. fluminea from each CBZ concentration group and control group were placed in 200-mL beakers that contained 100 mL of a neutral red solution (1 mg/L in dechlorinated tap water with no added CBZ) and were allowed to siphon for 2 h. Just prior to placing the C. fluminea in the solution and just after the 2 h siphoning period, 1-mL aliquots of the water were removed from each beaker, and the neutral red concentration was determined by measuring the optical density at 530 nm using a spectrophotometer.…”

Section: Siphoning Behaviormentioning

confidence: 99%

“…Immediately after CBZ exposure, five C. fluminea from each CBZ concentration group and control group were placed in 200-mL beakers that contained 100 mL of a neutral red solution (1 mg/L in dechlorinated tap water with no added CBZ) and were allowed to siphon for 2 h. Just prior to placing the C. fluminea in the solution and just after the 2 h siphoning period, 1-mL aliquots of the water were removed from each beaker, and the neutral red concentration was determined by measuring the optical density at 530 nm using a spectrophotometer. Standard solutions of neutral red were used to generate a standard curve from which the dye concentrations in each test sample were calculated using the equation of Cooper and Bidwell (2006):…”

Section: Siphoning Behaviormentioning

confidence: 99%

See 2 more Smart Citations

Effects of the human antiepileptic drug carbamazepine on the behavior, biomarkers, and heat shock proteins in the Asian clam Corbicula fluminea

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Section: Siphoning Behaviormentioning

confidence: 99%

Section: Siphoning Behaviormentioning

confidence: 99%

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Effects of the human antiepileptic drug carbamazepine on the behavior, biomarkers, and heat shock proteins in the Asian clam Corbicula fluminea

et al. 2014

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“…Nevertheless, some studies report lack of effect or irregular response of mollusks exposed to OPs and carbamates [21,27]. Moreover, Cooper and Bidwell [49] found that C. fluminea ChE is inhibited by concentrations of chlorpyrifos between 0.5 and 1 mg/L, Hypoxia induced GST activity when analysis was done towards ethacrynic acid instead of 1-chloro-2,4-dinitrobenzene (CDNB) as substrate. however, higher concentrations result in valve closure and no toxic effect is observed.…”

Section: Cholinesterasementioning

confidence: 99%

“…The examples of physiological and/or behavioral responses that endanger the test species described in Table 3 are related to high levels of ChE inhibition, thus providing an effective biomarker of conditions [49] a ChE ¼ cholinesterase.…”

Section: Biomarker Responses and Endpoints Of Higher Biological Organmentioning

confidence: 99%

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

130

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Abstract-Studies investigating the use of biomarkers in pesticide risk assessment have greatly increased in recent years; however, issues concerning the ecological meaning of enzymatic responses have proved controversial. Ideally a good biomarker response should be modulated by the environmental contaminants alone and demonstrate a predictable behavior towards certain types of toxins. As these premises are rarely observed, the present study aims to outline research that has contributed to an understanding of the behavior of two widely used biomarkers, cholinesterase and glutathione-S-transferase, describing environmental and biotic factors that affect their response in freshwater invertebrates. Studies were performed in the main classes of aquatic invertebrates with these biomarkers and conclusions were reached concerning their behavior towards the main classes of pesticides. Links between biomarker responses and conventional endpoints were evaluated so that ecological relevance could be attributed to enzymatic responses. Toxicity of mixtures was investigated, and cases of synergism and antagonism were pointed out as factors changing the expected toxicity of aquatic systems and leading to misinterpretations of biomarker responses. Finally, the use of biomarkers as a tool for biomonitoring and in situ assays was investigated, with discussion of advantages and disadvantages of their use.

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Contaminants of Concern and Spatiotemporal Metabolomic Changes in Quagga Mussels (Dreissena bugensis rostriformis) from the Milwaukee Estuary (Wisconsin, USA)

Fuller,

Kimbrough,

Davenport

et al. 2023

Enviro Toxic and Chemistry

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Environmental metabolomics has emerged as a promising technique in the field of biomonitoring and as an indicator of aquatic ecosystem health. In the Milwaukee Estuary (Wisconsin, USA), previous studies have used a non‐targeted metabolomic approach to distinguish between zebra mussels (Dreissena polymorpha) collected from sites of varying contamination. To further elucidate the potential effects of contaminants on bivalve health in the Milwaukee Estuary, the present study adopted a caging approach to study the metabolome of quagga mussels (Dreissena bugensis rostriformis) deployed in six sites of varying contamination for 2, 5 or 55 days. Caged mussels were co‐deployed with two types of passive sampler (POCIS and SPMDs) and data loggers. In conjunction, in‐situ quagga mussels were collected from the four sites studied previously and analyzed for residues of contaminants and metabolomics using a targeted approach. For the caging study, temporal differences in the metabolomic response were observed with few significant changes observed after 2 and 5 days, but larger differences (up to 97 significantly different metabolites) to the metabolome in all sites after 55 days. A suite of metabolic pathways were altered including biosynthesis and metabolism of amino acids, and up‐modulation of phospholipids at all sites, suggesting a potential biological influence such as gametogenesis. In the caging study, average temperatures appeared to have a greater effect on the metabolome than contaminants, despite a large concentration gradient in PAH residues measured in passive samplers and mussel tissue. Conversely, significant differences between the metabolome of mussels collected in situ from all three contaminated sites and the offshore reference site were observed. Overall, these findings highlight the importance of contextualizing the effects of environmental conditions and reproductive processes on the metabolome of model organisms to facilitate the wider use of this technique for biomonitoring and environmental health assessments.

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Cholinesterase inhibition and impacts on behavior of the Asian clam, Corbicula fluminea, after exposure to an organophosphate insecticide

Cited by 102 publications

References 38 publications

Effects of the human antiepileptic drug carbamazepine on the behavior, biomarkers, and heat shock proteins in the Asian clam Corbicula fluminea

Effects of the human antiepileptic drug carbamazepine on the behavior, biomarkers, and heat shock proteins in the Asian clam Corbicula fluminea

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Contaminants of Concern and Spatiotemporal Metabolomic Changes in Quagga Mussels (Dreissena bugensis rostriformis) from the Milwaukee Estuary (Wisconsin, USA)

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