Cholinesterases from the marine mussels Mytilus galloprovincialis Lmk. and M. edulis L. and from the freshwater bivalve Corbicula fluminea Müller

Mora, Pascal; Fournier, Didier; Narbonne, Jean‐François

doi:10.1016/s0742-8413(98)10130-5

Cited by 34 publications

(36 citation statements)

References 21 publications

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“…This proved the purification ability of the matrix to isolate ChE from the liver tissue of P. javanicus. ChE exists mostly in various native forms such as two molecular forms in Corbicula fluminea (Mora et al, 1999) and Pacu serum (Salles et al, 2006), three molecular forms in rat (Andres et al, 1990) and cotton aphid (Li and Han, 2002) while curimbata serum showed four ChE bands (Salles et al, 2006). In contrast, results with the purified ChE from P. javanicus obtained from non-denaturing PAGE showed only one major protein band after staining with commasie brilliant blue, suggesting a major molecular form of ChE in our purified sample.…”

Section: Discussionmentioning

confidence: 54%

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Sabullah¹,

Shukor²,

Shamaan³

et al. 2015

IJAB

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The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants.

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Section: Discussionmentioning

confidence: 54%

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Sabullah¹,

Shukor²,

Shamaan³

et al. 2015

IJAB

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“…ChE have already been characterized in several tissues of M. galloprovincialis, providing evidence that the occurring enzyme in gills and haemolymph is AChE (Mora et al, 1999;Moreira et al, 2001). AChE activity has been widely used as a biomarker of exposure to organophosphate and carbamate pesticides in several field studies (Escartı´n and Porte, 1997;Radenac et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

et al. 2004

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Abstract. In December 2000, the ship 'Coral Bulker' ran aground at the entrance of the port of Viana do Castelo (North-west coast of Portugal). A large amount of fuel oil was spilled and part of it reached the shore. To evaluate the spatial and temporal impact of this oil spill, a field study, and several laboratory toxicity tests were performed using Mytilus galloprovincialis as biological indicator of environmental contamination and the biomarkers glutathione S-transferases (GSTs) and acetylcholinesterase (AChE) as indicative criteria. Fifteen days after the oil spill, mussels collected at stations located near the ship presented higher and lower values of GSTs and AChE activity, respectively. These results, and those obtained in the laboratory toxicity tests, evidence that these biomarkers were sensitive indicators of exposure to this kind of pollution and were able to monitor a spatial impact of the oil spill of at least 10 km, confirming the higher level of contamination near the ship and a contamination gradient along the sampling stations. One year after the accident, such a contamination gradient was no longer evident. This study highlight the potential suitability of a biomarker approach for assessing spatial and temporal impacts of marine pollution accidents, such as fuel oil spills, suggesting the inclusion of these biomarkers in risk assessment studies, as cost-effective and early warning recognized tools. Major advantages and limitations of the biomarker approach used in this study are further discussed.

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“…This behavioral form of exposure avoidance should be considered when using bivalves to assess OP and carbamate contamination. Mora et al [50] studied freshwater and marine mussels and compared the sensitivity of the ChEs in bivalves with enzymes from various species representative of different phyla. They found ChEs of bivalves to be less sensitive than those from vertebrates, insects, and nematodes.…”

Section: Cholinesterasementioning

confidence: 99%

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

Domingues

Agra

Monaghan

et al. 2009

Enviro Toxic and Chemistry

130

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Abstract-Studies investigating the use of biomarkers in pesticide risk assessment have greatly increased in recent years; however, issues concerning the ecological meaning of enzymatic responses have proved controversial. Ideally a good biomarker response should be modulated by the environmental contaminants alone and demonstrate a predictable behavior towards certain types of toxins. As these premises are rarely observed, the present study aims to outline research that has contributed to an understanding of the behavior of two widely used biomarkers, cholinesterase and glutathione-S-transferase, describing environmental and biotic factors that affect their response in freshwater invertebrates. Studies were performed in the main classes of aquatic invertebrates with these biomarkers and conclusions were reached concerning their behavior towards the main classes of pesticides. Links between biomarker responses and conventional endpoints were evaluated so that ecological relevance could be attributed to enzymatic responses. Toxicity of mixtures was investigated, and cases of synergism and antagonism were pointed out as factors changing the expected toxicity of aquatic systems and leading to misinterpretations of biomarker responses. Finally, the use of biomarkers as a tool for biomonitoring and in situ assays was investigated, with discussion of advantages and disadvantages of their use.

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Cholinesterases from the marine mussels Mytilus galloprovincialis Lmk. and M. edulis L. and from the freshwater bivalve Corbicula fluminea Müller

Cited by 34 publications

References 21 publications

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

Cholinesterase and glutathione‐S‐transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination

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