Cholinoceptor-mediated control of catecholamine release from chromaffin cells in the American eel, Anguilla rostrata

Reid, Scott D.; Perry, Steve F.

doi:10.1007/bf00261301

Cited by 21 publications

(9 citation statements)

References 24 publications

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“…The dose of drug was chosen according to previous works of our research group with adrenergic and cholinergic agents whose standardization was done by means of dose/response curves (Sosa et al 2000, Casais et al 2001. Besides, the appropriate bibliography such as Reid & Perry (1995) was previously checked.…”

Section: Methodsmentioning

confidence: 99%

“…It has been demonstrated that in the sympathetic ganglia its preganglion neurotransmitter is acetylcholine (Klein & Burden 1988) and that it possesses nicotinic and muscarinic cholinergic receptors in the principal cells as well as muscarinic receptors in other neuronal populations (Matthews 1989, Reid & Perry 1995, Prud'Homme et al 1999. The stimulation of such receptors causes modifications in the steroids' liberation, in cyclic rats ) and prepubertal rats as well as in male rats (Zhu et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Pregnancy in rats is modulated by ganglionic cholinergic action

Casais¹,

Delgado²,

Sosa³

et al. 2006

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The control of ovarian steroidogenesis during pregnancy is mainly of endocrine origin. At present, there is little information about the influence of neural factors on the gestation physiology. The purpose of this work was to study the action of cholinergic agents in celiac ganglion upon the liberation of progesterone and ovarian androstenedione in the second half of pregnancy in rats. We used the ex vivo celiac ganglion-superior ovarian nerve-ovary integrated system (celiac ganglion-SON-ovary) that was incubated in buffer solution for 180 min, with the celiac ganglion and the ovary located in different compartments and linked by the SON.The results obtained indicate that the control values of ovarian androstenedione vary according to the pregnancy day analyzed. The addition of acetylcholine in ganglion decreased the liberation of both steroids on Day 15 whereas at the end of pregnancy it decreased the liberation of androstenedione without modifying progesterone. Due to the effect observed with atropine and hexametonium, acetylcholine action might occur through unspecific ganglionic pathways (Days 15 and 21) or through muscarinic ganglionic receptors (Days 19 and 20). Thus, we conclude that the cholinergic sympathetic system from the celiac ganglion might be a fine modulator of the pregnancy physiology.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pregnancy in rats is modulated by ganglionic cholinergic action

Casais¹,

Delgado²,

Sosa³

et al. 2006

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“…Previous research using rainbow trout has led to the development of a well-characterized in situ perfusion technique in which catecholamine secretion can be studied in whole animal preparations without major disturbances to the chromaffin tissue (Reid and Perry, 1995;Montpetit and Perry, 2000). This, along with a field stimulation technique which allows stimulation of the nerves that innervate the main population of chromaffin cells (Montpetit and Perry, 1999), forms a model with which in vivo catecholamine secretion can be simulated.…”

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confidence: 99%

Nitric oxide and the control of catecholamine secretion in rainbow trout Oncorhynchus mykiss

McNeill

Perry

2005

Journal of Experimental Biology

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SUMMARY An in situ saline-perfused posterior cardinal vein preparation was used to assess the role of nitric oxide (NO) in the regulation of basal and stimulus-evoked catecholamine secretion from rainbow trout Oncorhynchus mykiss chromaffin cells. Addition of the NO donor, sodium nitroprusside(SNP) to the inflowing perfusate abolished catecholamine secretion during electrical field stimulation, thereby establishing the potential for NO to act as a potent inhibitor of catecholamine release. A possible role for endogenously produced NO was established by demonstrating that stimulus-evoked(depolarizing levels of KCl or electrical field stimulation) catecholamine secretion was markedly stimulated in the presence of the nitric oxide synthase(NOS) inhibitors l-NAME and 7-NI. Although in vitroexperiments demonstrated that catecholamine degradation was enhanced by NO in a dose-dependent manner, the dominant factor contributing to the reduction in catecholamine appearance in the perfusate was specific inhibition of catecholamine secretion. Subsequent experiments were performed to identify the NOS isoform(s) contributing to the inhibition of stimulus-evoked catecholamine secretion. Inducible NOS (iNOS; an enzyme that can be activated in the absence of Ca2+), although present in the vicinity of the chromaffin cells(based on mRNA measurements), does not appear to play a role because stimulus-evoked NO production was eliminated during perfusion with Ca2+-free saline. The potential involvement of endothelial NOS(eNOS) was revealed by showing that hypoxic perfusate evoked NO production and corresponded with an inhibition of stimulus-evoked catecholamine secretion;chemical removal of the endothelium (using saponin) prevented the production of NO during hypoxia. However, because removal of the endothelium did not affect NO production during electrical field stimulation, it would appear that the neuronal form of NOS (nNOS) is the key isoform modulating catecholamine secretion from trout chromaffin cells.

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“…In fish, the secretory profile of catecholamines during cholinergic stimulation of chromaffin tissue features the release of both adrenaline and noradrenaline (Nilsson et al, 1976;Fritsche et al, 1993;Reid and Perry, 1995;Al-Kharrat et al, 1997;Gfell et al, 1997;Montpetit and Perry, 1999). By contrast, however, administration of a range of VIP and PACAP doses selectively causes the release of adrenaline from the chromaffin cells of all vertebrate species studied to date (Guo and Wakade, 1994;Montpetit and Perry, 2000).…”

mentioning

confidence: 99%

Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss)

Montpetit

Shahsavarani

Perry

2003

Journal of Experimental Biology

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SUMMARY The current model for the neuronal control of catecholamine release from piscine chromaffin cells advocates that the neurotransmitters vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are co-released with acetylcholine from preganglionic fibres upon nerve stimulation. Both VIP and PACAP elicit the secretion of exclusively adrenaline from rainbow trout chromaffin cells, which presumably arises from the activation of VPAC type receptors. Thus, the goals of the present study were (1) to localise VPAC receptors in the chromaffin cell fraction of the posterior cardinal vein (PCV) of trout and (2) to test the hypothesis that the selective secretion of adrenaline elicited by VIP could be explained by the absence of the VPAC receptors from the noradrenaline-containing cells. Fluorescent labelling of chromaffin cells using aldehyde-induced fluorescence of catecholamines and antisera raised against dopamineβ-hydroxylase (DβH) revealed a distinct layer of chromaffin cells lining the walls of the PCV. Furthermore, specific VIP-binding sites were demonstrated on chromaffin cells using a biotinylated VIP that was previously established as being bioactive. Although multiple labelling experiments revealed that a number of DβH-positive cells were immunonegative for phenylethanolamine N-methyl transferase (PNMT;noradrenaline-containing cells versus adrenaline-containing cells,respectively), labelling of VIP-binding sites was similar to that of DβH labelling, suggesting that all chromaffin cells possess VIP-binding sites. Pharmacological assessment of the VIP-binding sites indicated that they exhibited characteristics of VPAC receptors. Specifically, the labelling of VIP-binding sites was prevented after pre-treatment of PCV tissue sections with unlabelled VIP, PACAP or the specific VPAC receptor antagonist VIP 6-28. By contrast, sections pre-treated with the PAC1 receptor blocker PACAP 6-27 displayed normal labelling of VIP-binding sites. Finally, partial cDNA clones for the trout VPAC1 and VPAC2 receptor were obtained and sequenced. Tissue distribution experiments using RT-PCR revealed the presence of VPAC1 receptor mRNA but not that of the VPAC2 receptor in the PCV tissue. The results provide direct evidence that VIP and PACAP can elicit the secretion of adrenaline from the chromaffin tissue via specific VIP-binding sites that exhibit properties of VPAC receptors. However, the selective secretion of adrenaline by VIP or PACAP cannot be explained by a lack of VIP-binding sites on the noradrenaline-containing cells.

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Cholinoceptor-mediated control of catecholamine release from chromaffin cells in the American eel, Anguilla rostrata

Cited by 21 publications

References 24 publications

Pregnancy in rats is modulated by ganglionic cholinergic action

Pregnancy in rats is modulated by ganglionic cholinergic action

Nitric oxide and the control of catecholamine secretion in rainbow trout Oncorhynchus mykiss

Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss)

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